##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632000.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78703
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7442283013354	34.0	31.0	34.0	31.0	34.0
2	32.91898656976227	34.0	31.0	34.0	31.0	34.0
3	32.994155241858635	34.0	33.0	34.0	31.0	34.0
4	36.38838417849383	37.0	37.0	37.0	35.0	37.0
5	36.25437403910905	37.0	37.0	37.0	35.0	37.0
6	36.282022286316916	37.0	37.0	37.0	35.0	37.0
7	36.27110783578771	37.0	37.0	37.0	35.0	37.0
8	36.25204884184847	37.0	37.0	37.0	35.0	37.0
9	38.04391192203601	39.0	38.0	39.0	35.0	39.0
10	38.002655553155535	39.0	38.0	39.0	35.0	39.0
11	38.07011168570448	39.0	38.0	39.0	37.0	39.0
12	38.037673278019895	39.0	38.0	39.0	35.0	39.0
13	38.05045550995515	39.0	38.0	39.0	35.0	39.0
14	39.456526434824596	40.0	39.0	41.0	37.0	41.0
15	39.467783947244705	40.0	39.0	41.0	37.0	41.0
16	39.45347699579432	40.0	39.0	41.0	37.0	41.0
17	39.440491467923714	40.0	39.0	41.0	37.0	41.0
18	39.41551147986735	40.0	39.0	41.0	37.0	41.0
19	39.46177401115587	40.0	39.0	41.0	37.0	41.0
20	39.444392208683276	40.0	39.0	41.0	37.0	41.0
21	39.42299531148749	40.0	39.0	41.0	37.0	41.0
22	39.382768128279736	40.0	39.0	41.0	37.0	41.0
23	39.33456157960942	40.0	39.0	41.0	36.0	41.0
24	39.29841302110466	40.0	39.0	41.0	36.0	41.0
25	39.24044826753745	40.0	39.0	41.0	36.0	41.0
26	39.156804696136106	40.0	39.0	41.0	36.0	41.0
27	39.08424075321144	40.0	39.0	41.0	36.0	41.0
28	39.03611044051688	40.0	39.0	41.0	36.0	41.0
29	38.952200043200385	40.0	39.0	41.0	35.0	41.0
30	38.892990102029145	40.0	38.0	41.0	35.0	41.0
31	38.83872279328615	40.0	38.0	41.0	35.0	41.0
32	38.7396795547819	40.0	38.0	41.0	35.0	41.0
33	38.66250333532394	40.0	38.0	41.0	35.0	41.0
34	38.58814784696899	40.0	38.0	41.0	35.0	41.0
35	38.58377698435892	40.0	38.0	41.0	35.0	41.0
36	38.5446679287956	40.0	38.0	41.0	35.0	41.0
37	38.4930053492243	40.0	38.0	41.0	35.0	41.0
38	38.42084799817034	40.0	38.0	41.0	34.0	41.0
39	38.38302224819893	40.0	38.0	41.0	34.0	41.0
40	38.294690164288525	40.0	38.0	41.0	34.0	41.0
41	38.25199801786463	40.0	38.0	41.0	34.0	41.0
42	38.192940548644906	40.0	38.0	41.0	34.0	41.0
43	37.34576826804569	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	6.0
21	11.0
22	19.0
23	38.0
24	53.0
25	74.0
26	133.0
27	178.0
28	249.0
29	356.0
30	538.0
31	721.0
32	983.0
33	1282.0
34	1897.0
35	2795.0
36	4515.0
37	8146.0
38	20235.0
39	36471.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.01869052005641	18.10731484187388	12.101190551821404	26.772804086248303
2	20.22286316912951	20.51001867781406	32.54640865024205	26.720709502814376
3	20.671384826499626	21.689135102855037	29.296214883803668	28.34326518684167
4	15.87741255098281	15.62964562977269	32.639162420746345	35.853779398498155
5	16.342452003100263	34.049527972250104	33.094037076096214	16.513982948553423
6	35.72926063809512	32.80561096781571	15.550868454823833	15.91425993926534
7	29.851466907233526	29.098001346835574	20.540513068116844	20.51001867781406
8	27.500857654727266	31.67985972580461	20.022108432969517	20.79717418649861
9	26.4920015755435	14.32346924513678	19.37791443782321	39.80661474149651
10	17.883689312986796	25.794442397367316	32.590879636100276	23.73098865354561
11	36.722869522127496	20.73872660508494	20.668843627307727	21.869560245479843
12	22.227869331537555	24.40948883778255	27.90999072462295	25.45265110605695
13	30.888276177528173	19.18859509802676	23.76148304384839	26.161645680596674
14	23.976214375563828	20.555760263268237	23.47813933395169	31.98988602721624
15	26.423389197362233	26.498354573523248	21.44009758204897	25.638158647065552
16	26.339529624029577	25.152789601412906	22.936863906077278	25.570816868480232
17	25.00667064787873	25.687712031307576	24.137580524249394	25.168036796564298
18	24.494619010711155	24.608972974346592	25.5415930777734	25.35481493716885
19	25.29509675615923	24.762715525456464	25.253166969492902	24.6890207488914
20	25.41199191898657	24.170616113744074	25.253166969492902	25.16422499777645
21	25.577169866459982	24.52257220182204	24.498430809499002	25.40182712221897
22	25.54032247817745	24.324358664853946	24.42600663252989	25.709312224438712
23	25.499663291107073	24.344688258389134	24.3205468660661	25.835101584437698
24	25.96216154403263	24.479371815559762	24.20365170323876	25.35481493716885
25	25.837642783629594	23.51879852102207	24.663608756972415	25.979949938375917
26	25.511098687470618	24.75128012909292	24.581019783235707	25.156601400200756
27	25.85161937918504	24.24304029071319	24.18205151010762	25.723288819994156
28	24.870716491112155	24.239228491925342	25.136271806665565	25.753783210296938
29	25.001588249494937	24.685208950103554	24.776692121011905	25.5365106793896
30	24.680126551719756	25.090530221211388	24.966011460808353	25.2633317662605
31	25.832560385245795	24.43998322808533	24.43998322808533	25.287473158583534
32	24.91518747697038	24.363747252328373	24.75128012909292	25.969785141608327
33	24.647090962225075	24.234146093541543	25.27476716262404	25.84399578160934
34	25.348461939189104	23.804683430110668	24.940599468889367	25.906255161810858
35	25.472980699592135	23.697953064050925	25.41834491696632	25.41072131939062
36	24.849116297981016	24.246852089501036	24.91899927575823	25.985032336759716
37	25.007941247474687	23.128724445065625	25.817313190094403	26.046021117365285
38	24.16045131697648	23.230372412741573	26.948146830489307	25.66102943979264
39	24.46539522000432	22.91653431254209	26.910028842610828	25.708041624842764
40	23.629340685869664	22.53535443375729	27.95192051128928	25.88338436908377
41	23.202419221630688	22.643355399412986	28.293711802599645	25.86051357635668
42	23.432397748497515	22.441330063657038	28.25178201593332	25.87449017191212
43	22.770415359007913	22.49850704547476	28.289900003811795	26.44117759170553
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	12.0
4	21.0
5	21.0
6	23.0
7	25.0
8	29.0
9	33.0
10	48.5
11	64.0
12	64.0
13	98.0
14	132.0
15	176.0
16	220.0
17	236.5
18	253.0
19	253.0
20	323.5
21	394.0
22	432.5
23	471.0
24	592.0
25	713.0
26	713.0
27	816.5
28	920.0
29	1204.5
30	1489.0
31	1621.5
32	1754.0
33	1754.0
34	2039.5
35	2325.0
36	2686.5
37	3048.0
38	3415.5
39	3783.0
40	3783.0
41	4032.0
42	4281.0
43	4392.5
44	4504.0
45	4673.0
46	4842.0
47	4842.0
48	5138.0
49	5434.0
50	5638.5
51	5843.0
52	6220.0
53	6597.0
54	6597.0
55	6268.0
56	5939.0
57	5757.5
58	5576.0
59	5260.0
60	4944.0
61	4944.0
62	4514.0
63	4084.0
64	3698.0
65	3312.0
66	2860.5
67	2409.0
68	2409.0
69	2104.0
70	1799.0
71	1594.5
72	1390.0
73	1100.0
74	810.0
75	810.0
76	657.5
77	505.0
78	422.5
79	340.0
80	274.0
81	208.0
82	208.0
83	158.5
84	109.0
85	83.5
86	58.0
87	49.0
88	40.0
89	40.0
90	31.5
91	23.0
92	12.0
93	1.0
94	2.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	78703.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.00834783934539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.58422736683606	75.00095294969697
2	3.83336470292992	6.210690825000317
3	1.2187088274044795	2.9617676581578847
4	0.639939770374553	2.073618540589304
5	0.3795721187025535	1.5374255110986874
6	0.2666415709893971	1.296011587868315
7	0.1991969383273731	1.129563040798953
8	0.15527950310559005	1.006314879991868
9	0.15684798293493946	1.1435396363543955
>10	0.5568103394190351	7.121710735295986
>50	0.009410878976096368	0.518404635147326
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.11181276444354091	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	79	0.10037736807999695	TruSeq Adapter, Index 5 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.10037736807999695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0012705995959493286	0.0
23	0.0	0.0	0.0	0.0012705995959493286	0.0
24	0.0	0.0	0.0	0.006352997979746642	0.0
25	0.0	0.0	0.0	0.0088941971716453	0.0
26	0.0	0.0	0.0	0.01651779474734127	0.0
27	0.0	0.0	0.0	0.04828278464607448	0.0
28	0.0	0.0	0.0	0.10418916686784493	0.0
29	0.0	0.0	0.0	0.17534274424100732	0.0
30	0.0	0.0	0.0	0.2769907119169536	0.0
31	0.0	0.0	0.0	0.5133222367635287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGTA	35	2.3603607E-5	31.714287	9
GAGAGCA	25	0.0054656724	29.6	24
GGTATCA	55	6.138089E-7	26.90909	1
TCTTATA	50	8.9514215E-6	25.900002	37
GAGTATT	40	0.0019153339	23.125	11
TGAGTAT	40	0.0019153339	23.125	10
GAAAGCT	40	0.0019153339	23.125	4
GCTGAGT	60	3.6698402E-5	21.583334	8
GTATCAA	60	3.6698402E-5	21.583334	2
ATCTCGT	45	0.0037946994	20.555557	27
TCCGAGC	45	0.0037946994	20.555557	3
CACGAGA	45	0.0037946994	20.555557	11
TCTCCGA	45	0.0037946994	20.555557	1
CGAGACA	45	0.0037946994	20.555557	13
GAACAGA	45	0.0037946994	20.555557	6
TGAGCGA	45	0.0037946994	20.555557	21
CGTATGC	50	0.006978229	18.5	31
CTCGTAT	50	0.006978229	18.5	29
GACACTG	50	0.006978229	18.5	16
CTGAGCG	50	0.006978229	18.5	20
>>END_MODULE