FastQCFastQC Report
Fri 10 Feb 2017
ERR1631999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360185
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8410.23349112261754376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.2023959909490956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5030.13965045740383414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGC250.005491394529.610
GGTATCA3550.026.0563371
TCGCACG450.00382078520.55555513
GTATCAA4600.019.706522
GAACGGC851.2404562E-619.5882366
CTAGATA500.007025733618.53
CAGTACT500.007025733618.54
ATCGAAC500.007025733618.58
TCTTATA3750.017.75999837
AGAACGG852.7147184E-517.4117645
AACGGCC752.0623041E-417.2666667
TTATCCT650.001577087817.0769234
TCTATGG803.3745053E-416.18752
CCGGAAT700.002587861615.8571433
TACTGAT700.002587861615.8571437
CCAGTAC700.002587861615.8571433
ACGAGAA700.002587861615.85714336
CGGACAT1001.09005596E-414.821
GCGCGTG1001.09005596E-414.88
CGCGCGT1001.09005596E-414.87
Produced by FastQC (version 0.11.5)