##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631999.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360185
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192437219762066	31.0	31.0	34.0	30.0	34.0
2	31.385715673889806	31.0	31.0	34.0	30.0	34.0
3	31.473187389813567	31.0	31.0	34.0	30.0	34.0
4	35.292843955189696	37.0	35.0	37.0	33.0	37.0
5	34.27034718269778	35.0	35.0	37.0	30.0	37.0
6	34.21754098588225	35.0	35.0	37.0	30.0	37.0
7	34.2125324486028	35.0	35.0	37.0	30.0	37.0
8	34.14113858156225	35.0	35.0	37.0	30.0	37.0
9	35.683376598137066	37.0	35.0	39.0	30.0	39.0
10	35.37574302094757	37.0	34.0	39.0	30.0	39.0
11	35.56945458583783	37.0	35.0	39.0	30.0	39.0
12	35.482760248205786	37.0	35.0	39.0	30.0	39.0
13	35.614786845648766	37.0	35.0	39.0	30.0	39.0
14	36.522106695170535	38.0	36.0	40.0	31.0	41.0
15	36.45652650721157	38.0	35.0	40.0	31.0	41.0
16	36.41211877229757	38.0	35.0	40.0	31.0	41.0
17	36.371675666671294	38.0	35.0	40.0	30.0	41.0
18	36.363463220289574	38.0	35.0	40.0	30.0	41.0
19	36.40992545497453	38.0	35.0	40.0	30.0	41.0
20	36.34500881491456	38.0	35.0	40.0	30.0	41.0
21	36.321223815539234	38.0	35.0	40.0	30.0	41.0
22	36.26363951858073	38.0	35.0	40.0	30.0	41.0
23	36.1724724794203	38.0	34.0	40.0	30.0	41.0
24	36.087163540958116	38.0	34.0	40.0	30.0	41.0
25	35.98709552035759	38.0	34.0	40.0	29.0	41.0
26	35.799167094687455	38.0	34.0	40.0	29.0	41.0
27	35.65609617280009	38.0	34.0	40.0	28.0	41.0
28	35.527678831711484	38.0	34.0	40.0	27.0	41.0
29	35.39818981912073	38.0	34.0	40.0	27.0	41.0
30	35.27870122298263	38.0	34.0	40.0	27.0	41.0
31	35.150108971778394	38.0	34.0	40.0	27.0	40.0
32	35.00001388175521	38.0	33.0	40.0	27.0	40.0
33	34.88465649596735	37.0	33.0	39.0	26.0	40.0
34	34.781406777072895	37.0	33.0	39.0	26.0	40.0
35	34.72893374238239	37.0	33.0	39.0	26.0	40.0
36	34.66478892791204	37.0	33.0	39.0	25.0	40.0
37	34.58989963490984	37.0	33.0	39.0	25.0	40.0
38	34.49238308091675	37.0	33.0	39.0	25.0	40.0
39	34.40766828157752	37.0	33.0	39.0	25.0	40.0
40	34.2972389188889	37.0	33.0	39.0	25.0	40.0
41	34.223882171661785	37.0	32.0	39.0	24.0	40.0
42	34.15812984993823	37.0	32.0	39.0	24.0	40.0
43	33.194813776253866	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	3.0
15	18.0
16	15.0
17	46.0
18	89.0
19	150.0
20	263.0
21	414.0
22	737.0
23	1129.0
24	1703.0
25	2448.0
26	3558.0
27	4919.0
28	7096.0
29	9395.0
30	12555.0
31	16330.0
32	21060.0
33	27548.0
34	34436.0
35	44090.0
36	52427.0
37	56240.0
38	47260.0
39	16250.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.178838652359204	19.86923386593001	13.160459208462319	25.79146827324847
2	20.053305940003057	21.212154864861112	33.390618709829674	25.34392048530616
3	20.993656037869428	22.663908824631786	29.209711675944305	27.13272346155448
4	16.344933853436427	16.505684578758135	33.267903993780976	33.88147757402446
5	16.17696461540597	34.71493815678055	33.044685370018186	16.0634118577953
6	34.609159182086984	34.0863722809112	15.498424420783763	15.806044116218054
7	29.236364645945834	29.471799214292655	21.299887557782807	19.991948581978704
8	26.777628163304968	32.614906228743564	20.10439079917265	20.503074808778823
9	26.217082887960352	14.477282507600261	20.428668600857893	38.876966003581494
10	17.707566944764498	26.25872815358774	32.8150811388592	23.218623762788567
11	35.95180254591391	21.55836583977678	21.037800019434457	21.452031594874857
12	21.649707789052847	25.25702069769702	28.68137207268487	24.411899440565264
13	30.565959159876176	19.654899565501065	24.251981620556105	25.52715965406666
14	23.263600649666145	21.16717797798354	24.500742673903687	31.068478698446633
15	26.078265335869066	26.953926454460902	21.768813248747172	25.19899496092286
16	25.337812513014146	25.799242056165582	23.985729555645015	24.877215875175256
17	23.93880922303816	26.147118841706344	25.271180088010325	24.642891847245167
18	23.79749295500923	25.013257076224715	26.296486527756567	24.89276344100948
19	24.539056318280885	25.359745686244565	26.27177700348432	23.829420991990226
20	25.100434498938046	24.650387995058097	25.966933659091858	24.282243846912003
21	25.39694879020503	24.805586018296154	25.24119549675861	24.556269694740205
22	25.40638838374724	24.934963976845232	25.07822369060344	24.580423948804086
23	24.921359856740285	24.823077029859654	25.47191026833433	24.783652845065728
24	24.527950914113582	25.279786776239987	25.27340116884379	24.918861140802644
25	24.990213362577567	24.73589960714633	25.306717381345695	24.96716964893041
26	24.652886710995737	25.259519413634663	25.353637713952555	24.73395616141705
27	24.958840595804936	24.795868789649763	25.229812457487128	25.01547815705818
28	24.392742618376666	25.03852187070533	25.903355220234047	24.665380290683956
29	24.20089676138651	24.948845732054362	26.124908033371742	24.72534947318739
30	24.28196621180782	25.449977095103904	25.90057886919222	24.367477823896053
31	24.715076974332632	25.14069158904452	25.45303108124991	24.691200355372935
32	24.18451629023974	24.893596346322028	25.642100587198247	25.279786776239987
33	23.987950636478477	24.993544983827757	26.09658925274512	24.92191512694865
34	24.976331607368436	24.365534378166775	25.64015714146897	25.017976872995824
35	24.475477879423075	24.589863542346293	26.164609853269848	24.770048724960784
36	24.105390285547703	24.72534947318739	26.040229326596055	25.12903091466885
37	24.387467551397197	23.830531532406958	26.650193650485164	25.131807265710677
38	23.829420991990226	23.7661201882366	27.37565417771423	25.028804642058944
39	23.568443994058608	23.628135541457862	27.71575718033788	25.087663284145645
40	23.331898885295058	23.514305148743006	28.16858003525966	24.985215930702278
41	22.671960242653082	23.531518525202326	28.63750572622402	25.159015505920568
42	22.849091439121562	23.6211946638533	28.419562169440702	25.110151727584434
43	22.439024390243905	23.343281924566543	28.730235851021003	25.487457834168552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	53.5
2	49.0
3	100.5
4	152.0
5	152.0
6	181.5
7	211.0
8	212.0
9	213.0
10	310.5
11	408.0
12	408.0
13	719.5
14	1031.0
15	1446.0
16	1861.0
17	1827.0
18	1793.0
19	1793.0
20	2280.5
21	2768.0
22	2797.5
23	2827.0
24	3425.5
25	4024.0
26	4024.0
27	4595.0
28	5166.0
29	6172.0
30	7178.0
31	8471.5
32	9765.0
33	9765.0
34	11159.5
35	12554.0
36	14193.5
37	15833.0
38	17170.0
39	18507.0
40	18507.0
41	19550.0
42	20593.0
43	21238.5
44	21884.0
45	22859.5
46	23835.0
47	23835.0
48	24246.5
49	24658.0
50	25338.5
51	26019.0
52	26919.5
53	27820.0
54	27820.0
55	26783.5
56	25747.0
57	24792.0
58	23837.0
59	22034.5
60	20232.0
61	20232.0
62	18875.0
63	17518.0
64	15354.5
65	13191.0
66	11447.0
67	9703.0
68	9703.0
69	8444.5
70	7186.0
71	6238.0
72	5290.0
73	4261.0
74	3232.0
75	3232.0
76	2620.0
77	2008.0
78	1674.0
79	1340.0
80	1052.0
81	764.0
82	764.0
83	587.0
84	410.0
85	338.0
86	266.0
87	202.5
88	139.0
89	139.0
90	113.5
91	88.0
92	54.0
93	20.0
94	11.5
95	3.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	360185.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.411133053412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.63632919583357	70.020357427091
2	4.610295590273197	7.045558195278477
3	1.3030552195288452	2.9870377726608464
4	0.6440593853234654	1.9685322954500022
5	0.3903816703362974	1.4914752876840018
6	0.25890224733833256	1.1869808441518024
7	0.18019748832358054	0.9638365979328637
8	0.140974867940775	0.8617639513127794
9	0.1129475830580567	0.7767407517399425
>10	0.6729160359382125	9.445180832144718
>50	0.04483728526344454	2.240035528922032
>100	0.004009838518931515	0.43508275252173695
>500	0.0010935923233449587	0.577417763109834
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.23349112261754376	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.2023959909490956	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	503	0.13965045740383414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.7763510418257287E-4	0.0	0.0	0.0
4	0.0	2.7763510418257287E-4	0.0	0.0	0.0
5	0.0	2.7763510418257287E-4	0.0	0.0	0.0
6	0.0	2.7763510418257287E-4	0.0	0.0	0.0
7	0.0	2.7763510418257287E-4	0.0	0.0	0.0
8	0.0	2.7763510418257287E-4	0.0	0.0	0.0
9	0.0	2.7763510418257287E-4	0.0	0.0	0.0
10	0.0	2.7763510418257287E-4	0.0	0.0	0.0
11	0.0	2.7763510418257287E-4	0.0	0.0	0.0
12	0.0	2.7763510418257287E-4	0.0	0.0	0.0
13	0.0	2.7763510418257287E-4	0.0	0.0	0.0
14	0.0	2.7763510418257287E-4	0.0	0.0	0.0
15	0.0	2.7763510418257287E-4	0.0	0.0	0.0
16	0.0	2.7763510418257287E-4	0.0	5.552702083651457E-4	0.0
17	0.0	2.7763510418257287E-4	0.0	8.329053125477185E-4	0.0
18	0.0	2.7763510418257287E-4	0.0	8.329053125477185E-4	0.0
19	0.0	2.7763510418257287E-4	0.0	0.0011105404167302915	0.0
20	0.0	2.7763510418257287E-4	0.0	0.002221080833460583	0.0
21	0.0	2.7763510418257287E-4	0.0	0.0024987159376431557	0.0
22	0.0	2.7763510418257287E-4	0.0	0.0027763510418257285	0.0
23	0.0	5.552702083651457E-4	0.0	0.003331621250190874	0.0
24	0.0	5.552702083651457E-4	0.0	0.00583033718783403	0.0
25	0.0	5.552702083651457E-4	0.0	0.00777378291711204	0.0
26	0.0	5.552702083651457E-4	0.0	0.011383039271485486	0.0
27	0.0	5.552702083651457E-4	0.0	0.029706956147535293	0.0
28	0.0	5.552702083651457E-4	0.0	0.07940363979621583	0.0
29	0.0	8.329053125477185E-4	0.0	0.14881241584185906	0.0
30	0.0	8.329053125477185E-4	0.0	0.24182017574302095	0.0
31	0.0	8.329053125477185E-4	0.0	0.47614420367311244	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGC	25	0.0054913945	29.6	10
GGTATCA	355	0.0	26.056337	1
TCGCACG	45	0.003820785	20.555555	13
GTATCAA	460	0.0	19.70652	2
GAACGGC	85	1.2404562E-6	19.588236	6
CTAGATA	50	0.0070257336	18.5	3
CAGTACT	50	0.0070257336	18.5	4
ATCGAAC	50	0.0070257336	18.5	8
TCTTATA	375	0.0	17.759998	37
AGAACGG	85	2.7147184E-5	17.411764	5
AACGGCC	75	2.0623041E-4	17.266666	7
TTATCCT	65	0.0015770878	17.076923	4
TCTATGG	80	3.3745053E-4	16.1875	2
CCGGAAT	70	0.0025878616	15.857143	3
TACTGAT	70	0.0025878616	15.857143	7
CCAGTAC	70	0.0025878616	15.857143	3
ACGAGAA	70	0.0025878616	15.857143	36
CGGACAT	100	1.09005596E-4	14.8	21
GCGCGTG	100	1.09005596E-4	14.8	8
CGCGCGT	100	1.09005596E-4	14.8	7
>>END_MODULE