FastQCFastQC Report
Fri 10 Feb 2017
ERR1631997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268164
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25640.20218205216360027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23970.1890134083604329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17150.13523487498462344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.021.673641
CAGTTCG801.6168851E-518.59
TAAACCG609.2375354E-418.55
AACACCG701.21932324E-418.55
TCTTATA12050.016.42738537
GACGGAC1708.54925E-1116.323537
GATACTT3850.015.85714414
GCTTAGG3750.015.7866681
TAGGACA4700.015.7446814
GCGTTTT855.3646916E-415.23529515
GTATCAA17050.015.0821112
TAGGGGT1353.972782E-715.0740754
CAATACT1353.972782E-715.0740754
CAAGACG2109.094947E-1214.976194
ACTATAC1252.9591447E-614.7999993
TACTTGC4000.014.79999916
GTCCCGA1001.0935054E-414.7999991
CTTATAC7050.014.69503637
TATACTC908.274846E-414.3888885
CCAGATA4250.014.36470611
Produced by FastQC (version 0.11.5)