##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631997.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1268164
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8780378563025	34.0	31.0	34.0	31.0	34.0
2	33.04456048271359	34.0	33.0	34.0	31.0	34.0
3	33.11901142123574	34.0	33.0	34.0	31.0	34.0
4	36.47334177598481	37.0	37.0	37.0	35.0	37.0
5	36.35997236950426	37.0	37.0	37.0	35.0	37.0
6	36.38533817392703	37.0	37.0	37.0	35.0	37.0
7	36.37026835645863	37.0	37.0	37.0	35.0	37.0
8	36.36343958667806	37.0	37.0	37.0	35.0	37.0
9	38.150034222703056	39.0	39.0	39.0	37.0	39.0
10	38.14710557940456	39.0	38.0	39.0	37.0	39.0
11	38.19444882523081	39.0	39.0	39.0	37.0	39.0
12	38.17796042152277	39.0	39.0	39.0	37.0	39.0
13	38.183844518532304	39.0	39.0	39.0	37.0	39.0
14	39.6241511350267	41.0	39.0	41.0	37.0	41.0
15	39.64597875353661	41.0	39.0	41.0	37.0	41.0
16	39.63131030371466	41.0	39.0	41.0	37.0	41.0
17	39.62317965184314	41.0	39.0	41.0	37.0	41.0
18	39.612281219148315	41.0	39.0	41.0	37.0	41.0
19	39.6466080096896	41.0	40.0	41.0	37.0	41.0
20	39.62178708747449	41.0	39.0	41.0	37.0	41.0
21	39.59541668112326	41.0	39.0	41.0	37.0	41.0
22	39.555750675780104	41.0	39.0	41.0	37.0	41.0
23	39.510254982794024	41.0	39.0	41.0	37.0	41.0
24	39.467918975779156	41.0	39.0	41.0	37.0	41.0
25	39.42273712232803	41.0	39.0	41.0	37.0	41.0
26	39.33889307692065	41.0	39.0	41.0	36.0	41.0
27	39.269609451143545	40.0	39.0	41.0	36.0	41.0
28	39.20857239284509	40.0	39.0	41.0	36.0	41.0
29	39.16033967215597	40.0	39.0	41.0	36.0	41.0
30	39.0994981721607	40.0	39.0	41.0	35.0	41.0
31	39.02845136748875	40.0	39.0	41.0	35.0	41.0
32	38.94725997583909	40.0	39.0	41.0	35.0	41.0
33	38.87951085190874	40.0	38.0	41.0	35.0	41.0
34	38.80564185704688	40.0	38.0	41.0	35.0	41.0
35	38.77107298425125	40.0	38.0	41.0	35.0	41.0
36	38.73016029472529	40.0	38.0	41.0	35.0	41.0
37	38.67227188281642	40.0	38.0	41.0	35.0	41.0
38	38.612068312931136	40.0	38.0	41.0	35.0	41.0
39	38.55159900454515	40.0	38.0	41.0	35.0	41.0
40	38.48405726704117	40.0	38.0	41.0	35.0	41.0
41	38.4243102627105	40.0	38.0	41.0	35.0	41.0
42	38.36387407306941	40.0	38.0	41.0	34.0	41.0
43	37.54995331834053	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	1.0
15	2.0
16	3.0
17	6.0
18	15.0
19	38.0
20	61.0
21	142.0
22	269.0
23	423.0
24	689.0
25	1086.0
26	1724.0
27	2576.0
28	3702.0
29	5188.0
30	7357.0
31	9964.0
32	13449.0
33	18115.0
34	26666.0
35	40215.0
36	64467.0
37	119928.0
38	292303.0
39	659772.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.35485315779347	18.64908639576585	12.701511791850265	26.294548654590415
2	19.272980466248846	20.62485609116802	34.19265962446498	25.90950381811816
3	20.463047366113532	21.85245756857946	29.605871164928192	28.078623900378812
4	15.740629760819576	15.804580480127175	32.98437741490849	35.47041234414476
5	16.09602543519608	34.66744048876959	33.120321977283695	16.116212098750633
6	34.93964503013805	33.376597979441144	15.692607580722997	15.9911494096978
7	29.21073299667866	29.405423904163815	21.07424591772042	20.3095971814371
8	26.62368589551509	32.721871934544744	20.279001769487227	20.375440400452938
9	26.358499373898013	14.756451058380463	20.129494292536297	38.75555527518523
10	17.834207563059667	26.155292217725783	32.54184789979845	23.4686523194161
11	35.75696834163405	21.513621266650055	21.250327244741214	21.47908314697468
12	21.806091325727586	25.117886960992426	28.192016174564174	24.88400553871581
13	30.33148709472907	19.462940124463397	24.476487268208214	25.729085512599315
14	23.38483035317199	21.066597064732953	24.0199217135954	31.528650868499657
15	25.950902249235902	27.148144877160995	21.870436315807734	25.030516557795362
16	25.72932207506285	25.739651969303658	23.584962197318326	24.94606375831517
17	24.329581978356114	26.11373607829902	24.874700748483637	24.681981194861233
18	23.867023508000543	24.805624509132887	26.348721458738776	24.978630524127794
19	24.913970117429606	25.1152847738936	26.144016073630855	23.82672903504594
20	25.111420920322608	24.548402257121317	26.06011525323223	24.28006156932384
21	25.289079330433605	25.078933008664496	24.95568396516539	24.67630369573651
22	25.24570954545311	24.59681870799045	24.98478114817957	25.172690598376867
23	24.468838415220745	25.00275989540785	25.382048378600874	25.14635331077053
24	25.237193296766037	25.120016023164197	25.092890194012764	24.54990048605701
25	25.21259080055892	24.468680706911723	25.31171047277797	25.00701801975139
26	24.966013859406196	25.10708394182456	25.210304030078127	24.716598168691114
27	25.14793039386073	24.520882157197335	24.94669459155125	25.384492857390683
28	24.23101428521863	24.927138761232776	25.888449758863995	24.953397194684598
29	24.259796051614778	25.192009866231814	25.7597597787037	24.788434303449712
30	23.996817446323977	25.82725893496425	25.677830312167828	24.49809330654395
31	24.95552625685637	25.024287079589076	25.453963367513982	24.566223296040576
32	23.882242359820967	24.923196053507272	25.6010263656751	25.593535220996657
33	24.133077425317232	24.628912348876014	25.816455915796375	25.421554310010375
34	24.871625436457744	24.50313997243259	25.48440107115483	25.140833519954832
35	24.766355140186917	24.574266419800594	25.929927044136246	24.729451395876243
36	24.059979624086473	24.751609413293547	26.092760873199367	25.095650089420612
37	24.765882015259855	23.457612737784704	26.28067032339666	25.495834923558782
38	23.742512798029278	23.908737355736324	27.423267022246336	24.925482823988066
39	23.734706236732787	23.494753044558905	27.628445532281315	25.142095186426992
40	23.510129604688352	23.212849442185714	28.296971054216964	24.980049898908973
41	22.59502714159998	23.669730413416563	28.743127860434452	24.992114584549004
42	23.276090474102716	23.339252651865216	28.168281074056665	25.2163757999754
43	22.321639787913867	23.340277755873846	28.61538413012828	25.72269832608401
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	127.0
2	122.0
3	288.0
4	454.0
5	454.0
6	595.0
7	736.0
8	731.5
9	727.0
10	1091.5
11	1456.0
12	1456.0
13	2455.0
14	3454.0
15	5124.5
16	6795.0
17	6638.5
18	6482.0
19	6482.0
20	7833.0
21	9184.0
22	9671.5
23	10159.0
24	11752.5
25	13346.0
26	13346.0
27	15132.5
28	16919.0
29	21842.0
30	26765.0
31	29792.5
32	32820.0
33	32820.0
34	36931.0
35	41042.0
36	45539.0
37	50036.0
38	57284.5
39	64533.0
40	64533.0
41	69794.0
42	75055.0
43	74224.0
44	73393.0
45	77934.0
46	82475.0
47	82475.0
48	85743.5
49	89012.0
50	94193.0
51	99374.0
52	103525.0
53	107676.0
54	107676.0
55	98168.5
56	88661.0
57	84226.0
58	79791.0
59	73536.0
60	67281.0
61	67281.0
62	62941.5
63	58602.0
64	52752.5
65	46903.0
66	41312.0
67	35721.0
68	35721.0
69	30956.0
70	26191.0
71	23222.0
72	20253.0
73	16372.5
74	12492.0
75	12492.0
76	9993.0
77	7494.0
78	6309.0
79	5124.0
80	4164.5
81	3205.0
82	3205.0
83	2485.5
84	1766.0
85	1503.5
86	1241.0
87	1030.5
88	820.0
89	820.0
90	613.0
91	406.0
92	226.0
93	46.0
94	29.0
95	12.0
96	12.0
97	9.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1268164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.161760052800105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.95833343520472	49.70691390414558
2	7.542836909965259	8.623236671296768
3	2.024492651952934	3.4717068959877175
4	0.9066281025535157	2.0729783222115805
5	0.524622250857374	1.4994165610934562
6	0.3350300435940536	1.149054417744147
7	0.23009441882710277	0.920682137093834
8	0.1785885264746178	0.8166747598820193
9	0.1394628030488008	0.7174745355749823
>10	0.9043876679148504	10.289932104773055
>50	0.13201367511555756	5.3965431502117935
>100	0.11812753919342626	12.820758511608583
>500	0.004415979731548707	1.6489674479322192
>1k	9.659955662762795E-4	0.8656605804443251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2564	0.20218205216360027	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2397	0.1890134083604329	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1715	0.13523487498462344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5770830901996904E-4	0.0	0.0	0.0
3	0.0	1.5770830901996904E-4	0.0	7.885415450998452E-5	0.0
4	0.0	1.5770830901996904E-4	0.0	1.5770830901996904E-4	0.0
5	0.0	3.154166180399381E-4	0.0	1.5770830901996904E-4	0.0
6	0.0	3.154166180399381E-4	0.0	1.5770830901996904E-4	0.0
7	0.0	3.154166180399381E-4	0.0	1.5770830901996904E-4	0.0
8	0.0	3.154166180399381E-4	0.0	1.5770830901996904E-4	0.0
9	0.0	3.154166180399381E-4	0.0	1.5770830901996904E-4	0.0
10	0.0	3.942707725499226E-4	0.0	2.3656246352995355E-4	0.0
11	0.0	5.519790815698915E-4	0.0	2.3656246352995355E-4	0.0
12	0.0	5.519790815698915E-4	0.0	3.154166180399381E-4	0.0
13	0.0	5.519790815698915E-4	0.0	3.154166180399381E-4	0.0
14	0.0	5.519790815698915E-4	0.0	3.154166180399381E-4	0.0
15	0.0	5.519790815698915E-4	0.0	3.154166180399381E-4	0.0
16	0.0	5.519790815698915E-4	0.0	6.308332360798762E-4	0.0
17	0.0	5.519790815698915E-4	0.0	0.0014193747811797213	0.0
18	0.0	5.519790815698915E-4	0.0	0.0014982289356897057	0.0
19	0.0	5.519790815698915E-4	0.0	0.0017347913992196594	0.0
20	0.0	5.519790815698915E-4	0.0	0.0021290621717695818	0.0
21	0.0	5.519790815698915E-4	0.0	0.002759895407849458	0.0
22	0.0	5.519790815698915E-4	0.0	0.004336978498049148	0.0
23	0.0	5.519790815698915E-4	0.0	0.00575635327922887	0.0
24	0.0	5.519790815698915E-4	0.0	0.007491144678448529	0.0
25	0.0	5.519790815698915E-4	0.0	0.009462498541198142	0.0
26	0.0	5.519790815698915E-4	0.0	0.013641768730227322	0.0
27	0.0	5.519790815698915E-4	0.0	0.033828432284783355	0.0
28	0.0	6.308332360798762E-4	0.0	0.09147081923158204	0.0
29	0.0	6.308332360798762E-4	0.0	0.1639377872262578	0.0
30	0.0	6.308332360798762E-4	0.0	0.24933683656057104	0.0
31	0.0	6.308332360798762E-4	0.0	0.4903151327430837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1195	0.0	21.67364	1
CAGTTCG	80	1.6168851E-5	18.5	9
TAAACCG	60	9.2375354E-4	18.5	5
AACACCG	70	1.21932324E-4	18.5	5
TCTTATA	1205	0.0	16.427385	37
GACGGAC	170	8.54925E-11	16.32353	7
GATACTT	385	0.0	15.857144	14
GCTTAGG	375	0.0	15.786668	1
TAGGACA	470	0.0	15.744681	4
GCGTTTT	85	5.3646916E-4	15.235295	15
GTATCAA	1705	0.0	15.082111	2
TAGGGGT	135	3.972782E-7	15.074075	4
CAATACT	135	3.972782E-7	15.074075	4
CAAGACG	210	9.094947E-12	14.97619	4
ACTATAC	125	2.9591447E-6	14.799999	3
TACTTGC	400	0.0	14.799999	16
GTCCCGA	100	1.0935054E-4	14.799999	1
CTTATAC	705	0.0	14.695036	37
TATACTC	90	8.274846E-4	14.388888	5
CCAGATA	425	0.0	14.364706	11
>>END_MODULE