FastQCFastQC Report
Fri 10 Feb 2017
ERR1631996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573558
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18790.11941091462786882No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA16690.10606536270032627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.026.51
TAATAGA656.9042E-519.9230774
TCGTTTA1700.019.58823630
ATGGTCG1750.017.97142836
AATAACG2050.017.1463432
TAGCACT2500.017.024
TAACGCC2300.016.8913044
TAGGACA6000.016.654
ATACACT1452.9813236E-916.5862064
CGCATCG2250.016.44444513
CGTTTAT2050.016.24390431
TTAAGGT1151.2428318E-616.0869564
GCATCGC2300.016.08695614
AACGCCG2200.015.9772735
GTATCAA12450.015.7510042
ATAACGC2350.015.7446813
GCTTAGG6000.015.7251
GTTTATA5100.015.5980391
CTTAGGA6050.015.5950412
CGTATGC1201.936507E-615.41666631
Produced by FastQC (version 0.11.5)