##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631996.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1573558
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.138734002814004	33.0	31.0	34.0	30.0	34.0
2	32.33506613674234	34.0	31.0	34.0	31.0	34.0
3	32.42226915054926	34.0	31.0	34.0	31.0	34.0
4	36.03589445066531	37.0	35.0	37.0	35.0	37.0
5	35.69824245436139	37.0	35.0	37.0	33.0	37.0
6	35.691668181280896	37.0	35.0	37.0	33.0	37.0
7	35.670235860387734	37.0	35.0	37.0	33.0	37.0
8	35.637639667555945	37.0	35.0	37.0	33.0	37.0
9	37.345956107115214	39.0	37.0	39.0	34.0	39.0
10	37.216129942461606	39.0	37.0	39.0	34.0	39.0
11	37.31206984426377	39.0	37.0	39.0	34.0	39.0
12	37.25728762460615	39.0	37.0	39.0	34.0	39.0
13	37.29685909257873	39.0	37.0	39.0	34.0	39.0
14	38.52044093703569	40.0	38.0	41.0	35.0	41.0
15	38.50304342134195	40.0	38.0	41.0	34.0	41.0
16	38.4900664608486	40.0	38.0	41.0	34.0	41.0
17	38.461587053035224	40.0	38.0	41.0	34.0	41.0
18	38.447140175322424	40.0	38.0	41.0	34.0	41.0
19	38.48517372731097	40.0	38.0	41.0	34.0	41.0
20	38.45393051924365	40.0	38.0	41.0	34.0	41.0
21	38.42200605252555	40.0	38.0	41.0	34.0	41.0
22	38.38042512573416	40.0	38.0	41.0	34.0	41.0
23	38.32612080393605	40.0	38.0	41.0	34.0	41.0
24	38.26540489769046	40.0	38.0	41.0	34.0	41.0
25	38.19601501819444	40.0	38.0	41.0	34.0	41.0
26	38.089842255576215	40.0	38.0	41.0	34.0	41.0
27	37.99898065403372	40.0	38.0	41.0	33.0	41.0
28	37.92614444462804	40.0	37.0	41.0	33.0	41.0
29	37.85061306923545	40.0	37.0	41.0	33.0	41.0
30	37.76719637916111	40.0	37.0	41.0	33.0	41.0
31	37.67543935463453	39.0	37.0	41.0	33.0	41.0
32	37.57246380495666	39.0	37.0	40.0	33.0	41.0
33	37.4923650732925	39.0	37.0	40.0	32.0	41.0
34	37.41600818018783	39.0	37.0	40.0	32.0	41.0
35	37.38849028761571	39.0	37.0	40.0	32.0	41.0
36	37.347753308108125	39.0	36.0	40.0	32.0	41.0
37	37.29152659132997	39.0	36.0	40.0	32.0	41.0
38	37.23422651087535	39.0	36.0	40.0	32.0	41.0
39	37.174395224071816	39.0	36.0	40.0	31.0	41.0
40	37.11279787589653	39.0	36.0	40.0	31.0	41.0
41	37.06065998202799	39.0	36.0	40.0	31.0	41.0
42	37.0141087903973	39.0	36.0	40.0	31.0	41.0
43	36.068500176034185	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	0.0
12	0.0
13	3.0
14	1.0
15	7.0
16	17.0
17	31.0
18	64.0
19	126.0
20	260.0
21	466.0
22	809.0
23	1282.0
24	2224.0
25	3309.0
26	4841.0
27	6805.0
28	10308.0
29	14226.0
30	19539.0
31	26061.0
32	35115.0
33	48206.0
34	67423.0
35	99769.0
36	159338.0
37	276316.0
38	480882.0
39	316126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.646918639160425	18.3102878953302	12.24772140588399	26.795072059625387
2	18.863556348097752	20.941776534452494	33.64508966304388	26.549577454405878
3	19.766668912108738	22.030265169761776	30.191006623206775	28.012059294922715
4	15.249835087108323	15.509755598459032	34.06553809900874	35.1748712154239
5	15.462728415476265	35.751971010919206	33.56488925098407	15.220411322620455
6	35.06759839802537	34.84269407292263	15.491580227738666	14.59812730131333
7	30.18414319650118	30.17378450619551	20.488663271388788	19.15340902591452
8	27.99947634596246	32.96688142413562	19.798189834756645	19.23545239514527
9	27.325716624363384	14.066656583360764	18.374473645076954	40.2331531471989
10	16.952600412568206	26.57925541988284	32.41768018719361	24.050463980355346
11	37.16888732414058	20.854839796181647	20.95785474701282	21.018418132664955
12	22.656235105410797	24.515905991390213	28.062963042989203	24.764895860209794
13	31.15150506050619	18.51682619897074	24.187478313478117	26.14419042704495
14	23.56023737288362	19.624125707473127	23.7801212284517	33.035515691191556
15	26.147050188172283	26.75903906942102	21.166553759060676	25.927356983346023
16	26.395150353530028	25.411646726717414	22.465775014330582	25.72742790542198
17	25.194813283018487	25.59143037625559	23.995302365721503	25.21845397500442
18	24.867847260793692	24.318582473604405	25.20491777233505	25.608652493266852
19	26.11133494920429	24.512792029273786	24.859839929637168	24.51603309188476
20	26.266079801316504	23.87087098155899	24.608943553399364	25.254105663725134
21	26.15664627551066	24.743225225889354	23.634527611946936	25.46560088665305
22	26.34373820348535	24.271237539385265	23.76709342776053	25.617930829368856
23	25.46038976637658	24.398782885664207	24.086878272043357	26.053949075915856
24	26.00844709886766	24.425791740755663	23.98030450736484	25.585456653011835
25	26.054457477894044	23.832677282947305	24.27244499408347	25.840420245075173
26	25.77413733716838	24.41416204550452	24.019197258696533	25.79250335863057
27	25.75214895161157	24.170192646219586	24.01868885671834	26.0589695454505
28	25.146070243359315	24.387661592391254	24.64815405596743	25.818114108281996
29	24.996981363254484	24.767310769606205	24.58460380869342	25.65110405844589
30	24.841601008669524	25.47112975816589	24.258718140672286	25.428551092492302
31	25.69126781472307	24.711386552005074	24.065525388959287	25.53182024431257
32	24.848909287106036	24.647391453000147	24.458583668349053	26.045115591544764
33	24.92154721974023	24.251727613472145	24.739094459816542	26.087630706971083
34	25.557875845694916	24.054531196180882	24.681772136775386	25.70582082134882
35	25.45378054066008	24.23393354423542	24.985605868992437	25.32668004611206
36	24.94512436147889	24.427888898915707	24.86327164298996	25.763715096615442
37	25.71745051659996	23.053678351862466	25.089192772049074	26.139678359488492
38	24.740556115503846	23.521408171799195	26.077653318149064	25.6603823945479
39	24.768073372573493	23.20403823691278	26.152515509437848	25.87537288107588
40	24.2577013367159	22.860104298665824	27.144598419632448	25.73759594498582
41	23.501516944402432	22.920286382834316	27.83278404736273	25.745412625400526
42	24.06126752239193	22.316177732247557	27.70924236666205	25.913312378698468
43	22.882601086200825	22.028676413579927	28.44852239320063	26.640200107018618
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	32.5
2	41.0
3	96.0
4	151.0
5	151.0
6	209.5
7	268.0
8	291.0
9	314.0
10	449.0
11	584.0
12	584.0
13	893.0
14	1202.0
15	2378.0
16	3554.0
17	3721.5
18	3889.0
19	3889.0
20	4821.5
21	5754.0
22	7112.0
23	8470.0
24	10473.0
25	12476.0
26	12476.0
27	14540.0
28	16604.0
29	21884.0
30	27164.0
31	31234.5
32	35305.0
33	35305.0
34	41160.0
35	47015.0
36	53032.0
37	59049.0
38	67685.5
39	76322.0
40	76322.0
41	83528.5
42	90735.0
43	90695.0
44	90655.0
45	97532.5
46	104410.0
47	104410.0
48	109573.5
49	114737.0
50	120295.0
51	125853.0
52	133360.5
53	140868.0
54	140868.0
55	129072.5
56	117277.0
57	112707.0
58	108137.0
59	100531.0
60	92925.0
61	92925.0
62	86559.0
63	80193.0
64	71984.0
65	63775.0
66	55707.5
67	47640.0
68	47640.0
69	40816.5
70	33993.0
71	29735.0
72	25477.0
73	20655.5
74	15834.0
75	15834.0
76	12508.0
77	9182.0
78	7551.5
79	5921.0
80	4724.5
81	3528.0
82	3528.0
83	2727.0
84	1926.0
85	1551.5
86	1177.0
87	937.0
88	697.0
89	697.0
90	518.0
91	339.0
92	204.0
93	69.0
94	44.5
95	20.0
96	20.0
97	11.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1573558.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.712034596327484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.52441603416733	49.0697567555673
2	7.601873147456351	8.622353858708749
3	2.126177510762151	3.6173955764482972
4	1.0410014873314208	2.3614924945747164
5	0.5801040367536812	1.6449440100922
6	0.3605424981151095	1.2268259175930258
7	0.2625666995392298	1.0423484223678625
8	0.18378716543728726	0.8338355267712318
9	0.14947186586783814	0.7629168265447013
>10	0.9306440588471508	10.190892721111002
>50	0.12337485120760823	4.938464031836933
>100	0.10774411139545029	12.071289781241386
>500	0.006951149370281343	2.6342385017053003
>1k	0.0013453837490867116	0.9832455754373826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1879	0.11941091462786882	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1669	0.10606536270032627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.355024727401214E-5	0.0
3	0.0	0.0	0.0	1.2710049454802428E-4	0.0
4	0.0	0.0	0.0	1.2710049454802428E-4	0.0
5	0.0	0.0	0.0	1.2710049454802428E-4	0.0
6	0.0	0.0	0.0	1.2710049454802428E-4	0.0
7	0.0	0.0	0.0	1.2710049454802428E-4	0.0
8	0.0	0.0	0.0	1.2710049454802428E-4	0.0
9	0.0	0.0	0.0	2.5420098909604855E-4	0.0
10	0.0	0.0	0.0	3.813014836440729E-4	0.0
11	0.0	0.0	0.0	5.084019781920971E-4	0.0
12	0.0	0.0	0.0	5.719522254661092E-4	0.0
13	0.0	0.0	0.0	5.719522254661092E-4	0.0
14	0.0	0.0	0.0	5.719522254661092E-4	0.0
15	0.0	0.0	0.0	7.626029672881458E-4	0.0
16	0.0	0.0	0.0	0.0010803542036582064	0.0
17	0.0	0.0	0.0	0.0012710049454802428	0.0
18	0.0	0.0	0.0	0.001398105440028267	0.0
19	0.0	0.0	0.0	0.001398105440028267	0.0
20	0.0	0.0	0.0	0.0016523064291243158	0.0
21	0.0	0.0	0.0	0.0019700576654943765	0.0
22	0.0	0.0	0.0	0.0023513591491384493	0.0
23	0.0	0.0	0.0	0.003050411869152583	0.0
24	0.0	0.0	0.0	0.004067215825536777	0.0
25	0.0	0.0	0.0	0.004893369040098935	0.0
26	0.0	0.0	0.0	0.006672775963771275	0.0
27	0.0	0.0	0.0	0.01811182047309346	0.0
28	0.0	0.0	0.0	0.062152141833983876	0.0
29	0.0	6.355024727401214E-5	0.0	0.12818084875168248	0.0
30	0.0	6.355024727401214E-5	0.0	0.20850836130603384	0.0
31	0.0	6.355024727401214E-5	0.0	0.4496179994636359	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	26.5	1
TAATAGA	65	6.9042E-5	19.923077	4
TCGTTTA	170	0.0	19.588236	30
ATGGTCG	175	0.0	17.971428	36
AATAACG	205	0.0	17.146343	2
TAGCACT	250	0.0	17.02	4
TAACGCC	230	0.0	16.891304	4
TAGGACA	600	0.0	16.65	4
ATACACT	145	2.9813236E-9	16.586206	4
CGCATCG	225	0.0	16.444445	13
CGTTTAT	205	0.0	16.243904	31
TTAAGGT	115	1.2428318E-6	16.086956	4
GCATCGC	230	0.0	16.086956	14
AACGCCG	220	0.0	15.977273	5
GTATCAA	1245	0.0	15.751004	2
ATAACGC	235	0.0	15.744681	3
GCTTAGG	600	0.0	15.725	1
GTTTATA	510	0.0	15.598039	1
CTTAGGA	605	0.0	15.595041	2
CGTATGC	120	1.936507E-6	15.416666	31
>>END_MODULE