FastQCFastQC Report
Fri 10 Feb 2017
ERR1631995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810446
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.15435945146252805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9600.11845329608635244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8270.10204257902438904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA200.00184161637.032
ATTCGCG250.005495429.59999830
GGTATCA4900.027.1836721
CATAATT400.001930698623.1254
CTAGAAC1950.022.7692283
GTCTAGA1900.021.4210511
CTTATAC7250.019.64827537
TAGAACT2550.019.5882364
AATCTAC1103.8473445E-818.514
AGATAAT500.00703313718.49999830
TCGTAGC500.00703313718.4999982
TCTAGAC2250.018.0888883
TTACACT852.7215114E-517.4117644
TTAGGAC752.066318E-417.2666663
TTTCGGA1502.5102054E-1017.26666630
GTATCAA7300.017.2328762
GTTCTAG2150.017.2093031
GAATCTA1201.0400436E-716.95833213
CGGTCCA1005.8734895E-616.6510
TCGTTTA1452.9740477E-916.58620830
Produced by FastQC (version 0.11.5)