FastQCFastQC Report
Fri 10 Feb 2017
ERR1631994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145850
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC2560.17552279739458349No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1560.10695920466232431No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC1470.10078848131642097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG352.3741415E-531.7142871
ATAAACC250.00548081829.5999983
TTACACA250.00548081829.5999984
ATCGCCT250.00548081829.59999832
TGCATCA502.6864608E-422.19999914
GCTGTAC603.697654E-521.5833323
CTCTATG450.003810053520.5555551
TGTACCA656.8494715E-519.9230775
GTACCAG759.17515E-619.7333356
CTGTACC701.2099613E-418.54
TCTTATA1409.094947E-1118.537
TGCTGTA609.184214E-418.52
TAATTTG500.007006190318.4999984
TCTTAGC650.001571010317.0769232
CTCAGAA650.001571010317.0769232
TCCAGGA1403.4013283E-815.8571432
GCATCAG700.002577970515.85714315
CATCAAG956.992368E-515.57894829
CAGGACA855.3243816E-415.2352954
GCGGGTG1353.9031693E-715.07407513
Produced by FastQC (version 0.11.5)