##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631994.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145850
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79282824820021	34.0	31.0	34.0	31.0	34.0
2	32.97090161124443	34.0	31.0	34.0	31.0	34.0
3	33.052190606787796	34.0	33.0	34.0	31.0	34.0
4	36.42094617757971	37.0	37.0	37.0	35.0	37.0
5	36.296900925608504	37.0	37.0	37.0	35.0	37.0
6	36.325368529310936	37.0	37.0	37.0	35.0	37.0
7	36.30986630099417	37.0	37.0	37.0	35.0	37.0
8	36.29268426465547	37.0	37.0	37.0	35.0	37.0
9	38.09963661295852	39.0	39.0	39.0	37.0	39.0
10	38.06844703462461	39.0	38.0	39.0	35.0	39.0
11	38.132979088104214	39.0	38.0	39.0	37.0	39.0
12	38.09863558450463	39.0	38.0	39.0	37.0	39.0
13	38.10776139869729	39.0	38.0	39.0	37.0	39.0
14	39.535755913609876	41.0	39.0	41.0	37.0	41.0
15	39.538121357559135	41.0	39.0	41.0	37.0	41.0
16	39.53541995200548	41.0	39.0	41.0	37.0	41.0
17	39.51114844017827	41.0	39.0	41.0	37.0	41.0
18	39.48508741858073	41.0	39.0	41.0	37.0	41.0
19	39.50879670894755	41.0	39.0	41.0	37.0	41.0
20	39.47363729859445	41.0	39.0	41.0	37.0	41.0
21	39.457867672266026	41.0	39.0	41.0	37.0	41.0
22	39.42286595817621	41.0	39.0	41.0	37.0	41.0
23	39.37435721631814	40.0	39.0	41.0	36.0	41.0
24	39.33026396983202	41.0	39.0	41.0	36.0	41.0
25	39.27779910867329	40.0	39.0	41.0	36.0	41.0
26	39.18595817620843	40.0	39.0	41.0	36.0	41.0
27	39.11223860130271	40.0	39.0	41.0	36.0	41.0
28	39.05357559136099	40.0	39.0	41.0	35.0	41.0
29	38.98697291738087	40.0	39.0	41.0	35.0	41.0
30	38.927980802194035	40.0	39.0	41.0	35.0	41.0
31	38.8634830305108	40.0	38.0	41.0	35.0	41.0
32	38.78374357216318	40.0	38.0	41.0	35.0	41.0
33	38.69568049365787	40.0	38.0	41.0	35.0	41.0
34	38.60793280767912	40.0	38.0	41.0	35.0	41.0
35	38.5857867672266	40.0	38.0	41.0	35.0	41.0
36	38.55516626671238	40.0	38.0	41.0	35.0	41.0
37	38.49924580047995	40.0	38.0	41.0	34.0	41.0
38	38.45075077134042	40.0	38.0	41.0	34.0	41.0
39	38.38974974288653	40.0	38.0	41.0	34.0	41.0
40	38.298738429893724	40.0	38.0	41.0	34.0	41.0
41	38.22848817278025	40.0	38.0	41.0	34.0	41.0
42	38.19253342475146	40.0	37.0	41.0	34.0	41.0
43	37.31866300994172	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	5.0
20	7.0
21	20.0
22	31.0
23	52.0
24	97.0
25	151.0
26	205.0
27	307.0
28	465.0
29	667.0
30	871.0
31	1217.0
32	1676.0
33	2355.0
34	3579.0
35	5476.0
36	8373.0
37	14770.0
38	35902.0
39	69622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.720946177579705	17.035995886184434	11.560507370586219	27.68255056564964
2	21.042166609530337	18.673294480630783	32.32430579362359	27.96023311621529
3	21.863558450462804	20.034967432293453	27.87589989715461	30.225574220089136
4	16.773397326019886	14.791909496057592	30.909153239629756	37.525539938292766
5	17.960918752142614	32.89406924922866	31.812821391840934	17.332190606787794
6	37.81830647925951	31.23071648954405	14.53753856702091	16.413438464175524
7	31.687350017140897	27.092218032224892	19.6085018854988	21.61193006513541
8	28.64175522797395	30.558793280767908	18.909153239629756	21.890298251628383
9	27.52485430236544	13.453548165923895	18.33527596846075	40.68632156324992
10	19.1470689064107	24.78025368529311	30.86664381213576	25.206033596160438
11	38.33801851217004	19.965032567706547	19.326705519369213	22.3702434007542
12	23.958176208433322	23.076448405896468	26.590332533424753	26.375042852245457
13	32.421666095303394	17.797737401439836	21.983544737744257	27.797051765512514
14	24.570449091532396	19.212889955433667	22.33321906067878	33.88344189235516
15	27.940349674322935	24.933836133013372	19.830647925951318	27.29516626671238
16	27.389784024682896	23.476174151525537	21.305450805622215	27.82859101816935
17	25.943092218032227	24.348303051079874	23.1319849160096	26.5766198148783
18	26.087075762769967	22.527254028111074	23.837504285224544	27.548165923894413
19	27.1107302022626	22.929722317449432	23.446006170723347	26.51354130956462
20	26.459376071306135	23.112787110044568	23.424751456976345	27.00308536167295
21	28.078162495714775	22.681522111758657	22.625985601645525	26.61432979088104
22	28.37915666780939	22.575248543023655	22.24340075419952	26.80219403496743
23	27.341103873842986	22.734316078162493	22.402468289338362	27.522111758656152
24	27.18752142612273	22.867329448063078	22.62118615015427	27.323962975659928
25	27.322591703805276	22.641069592046623	22.885841618100788	27.15049708604731
26	27.321906067877954	22.959204662324307	22.90709633184779	26.811792937949946
27	27.48782996229002	22.71511827219746	22.508741858073364	27.28830990743915
28	26.708947548851558	22.527939664038396	23.437778539595474	27.325334247514572
29	26.786424408639014	22.680150839904012	23.38841275282825	27.145011998628725
30	26.307850531367844	22.972917380870758	23.49194377785396	27.227288309907436
31	26.710318820706203	22.9256085018855	22.981830647925953	27.382242029482345
32	26.25848474460062	22.958519026396985	23.41789509770312	27.36510113129928
33	26.792595131984914	22.80150839904011	23.118272197463146	27.287624271511827
34	27.131299280082278	22.752142612272884	23.248543023654438	26.8680150839904
35	27.05862187178608	22.52382584847446	23.774425779910867	26.64312649982859
36	26.368186492972235	22.942063764141242	23.3383613301337	27.35138841275283
37	25.992458004799452	21.692835104559478	24.29825162838533	28.01645526225574
38	26.0699348645869	22.00479945149126	25.14569763455605	26.779568049365785
39	25.35138841275283	21.718203633870413	25.922523140212544	27.007884813164214
40	25.448063078505314	21.046280425094274	26.763798423037365	26.741858073363044
41	24.41001028453891	21.19300651354131	27.095646211861503	27.301336990058278
42	24.13781282139184	21.87521426122729	26.66232430579362	27.32464861158725
43	23.917038052793966	20.874871443263626	27.19094960575934	28.01714089818306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	7.0
2	4.0
3	7.5
4	11.0
5	11.0
6	11.0
7	11.0
8	14.0
9	17.0
10	30.0
11	43.0
12	43.0
13	62.5
14	82.0
15	117.0
16	152.0
17	160.0
18	168.0
19	168.0
20	241.5
21	315.0
22	373.5
23	432.0
24	569.5
25	707.0
26	707.0
27	907.5
28	1108.0
29	1388.0
30	1668.0
31	2097.0
32	2526.0
33	2526.0
34	2929.0
35	3332.0
36	3970.5
37	4609.0
38	5082.5
39	5556.0
40	5556.0
41	6014.0
42	6472.0
43	6956.0
44	7440.0
45	7847.0
46	8254.0
47	8254.0
48	8699.0
49	9144.0
50	9724.5
51	10305.0
52	10909.0
53	11513.0
54	11513.0
55	11640.0
56	11767.0
57	11541.5
58	11316.0
59	10720.0
60	10124.0
61	10124.0
62	9928.5
63	9733.0
64	8519.0
65	7305.0
66	6471.0
67	5637.0
68	5637.0
69	5050.5
70	4464.0
71	3959.0
72	3454.0
73	3422.5
74	3391.0
75	3391.0
76	3039.0
77	2687.0
78	1919.0
79	1151.0
80	797.5
81	444.0
82	444.0
83	343.5
84	243.0
85	193.5
86	144.0
87	105.0
88	66.0
89	66.0
90	51.5
91	37.0
92	20.0
93	3.0
94	3.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	145850.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.40490384798971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22366437433602	67.13083072585766
2	5.432049573035247	8.083422523584021
3	1.5870669779919906	3.54256697693441
4	0.7675856562043505	2.2844854777992314
5	0.44270155108229864	1.6469583170817166
6	0.32277759172703263	1.4409741406041325
7	0.2008612660549504	1.0461544231322844
8	0.15489298780083777	0.9219838291239341
9	0.09293579079805998	0.6223390720529887
>10	0.7205022042919053	9.926940179452014
>50	0.04197100218995318	2.087103668868733
>100	0.01299102448736646	1.2662406655088896
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	256	0.17552279739458349	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	156	0.10695920466232431	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	147	0.10078848131642097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0013712718546451835	0.0
25	0.0	0.0	0.0	0.0034281796366129585	0.0
26	0.0	0.0	0.0	0.0075419952005485085	0.0
27	0.0	0.0	0.0	0.04045251971203291	0.0
28	0.0	0.0	0.0	0.10490229688035653	0.0
29	0.0	0.0	0.0	0.18649297223174494	0.0
30	0.0	0.0	0.0	0.28522454576619816	0.0
31	0.0	0.0	0.0	0.5183407610558793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAG	35	2.3741415E-5	31.714287	1
ATAAACC	25	0.005480818	29.599998	3
TTACACA	25	0.005480818	29.599998	4
ATCGCCT	25	0.005480818	29.599998	32
TGCATCA	50	2.6864608E-4	22.199999	14
GCTGTAC	60	3.697654E-5	21.583332	3
CTCTATG	45	0.0038100535	20.555555	1
TGTACCA	65	6.8494715E-5	19.923077	5
GTACCAG	75	9.17515E-6	19.733335	6
CTGTACC	70	1.2099613E-4	18.5	4
TCTTATA	140	9.094947E-11	18.5	37
TGCTGTA	60	9.184214E-4	18.5	2
TAATTTG	50	0.0070061903	18.499998	4
TCTTAGC	65	0.0015710103	17.076923	2
CTCAGAA	65	0.0015710103	17.076923	2
TCCAGGA	140	3.4013283E-8	15.857143	2
GCATCAG	70	0.0025779705	15.857143	15
CATCAAG	95	6.992368E-5	15.578948	29
CAGGACA	85	5.3243816E-4	15.235295	4
GCGGGTG	135	3.9031693E-7	15.074075	13
>>END_MODULE