FastQCFastQC Report
Fri 10 Feb 2017
ERR1631993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA281.5126958400864399TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110.5942733657482442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3241491085899514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60.3241491085899514No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAACA50.2701242571582928TruSeq Adapter, Index 25 (96% over 27bp)
AGATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAA30.1620745542949757TruSeq Adapter, Index 25 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1620745542949757No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAATA30.1620745542949757TruSeq Adapter, Index 25 (96% over 27bp)
GATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30.1620745542949757TruSeq Adapter, Index 25 (96% over 27bp)
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC30.1620745542949757TruSeq Adapter, Index 25 (96% over 27bp)
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT30.1620745542949757TruSeq Adapter, Index 25 (96% over 27bp)
TCCTGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAA20.10804970286331712RNA PCR Primer, Index 40 (96% over 30bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG20.10804970286331712No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTT20.10804970286331712No Hit
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCG20.10804970286331712No Hit
GAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCAC20.10804970286331712No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCG20.10804970286331712No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA20.10804970286331712No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAATGA20.10804970286331712TruSeq Adapter, Index 25 (96% over 27bp)
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA20.10804970286331712No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20.10804970286331712No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG20.10804970286331712No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAATT20.10804970286331712TruSeq Adapter, Index 25 (96% over 27bp)
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG20.10804970286331712No Hit
GACCAGAGGAGGTCACACATGGGCCCATCATGAGGCACCTCTT20.10804970286331712No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA20.10804970286331712No Hit
TCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA20.10804970286331712Illumina PCR Primer Index 6 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)