##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631993.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1851
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65532144786602	31.0	31.0	34.0	30.0	34.0
2	31.874122096164236	33.0	31.0	34.0	30.0	34.0
3	31.888708806050783	33.0	31.0	34.0	30.0	34.0
4	35.62668827660724	37.0	35.0	37.0	33.0	37.0
5	35.319827120475416	37.0	35.0	37.0	33.0	37.0
6	35.30415991356024	37.0	35.0	37.0	33.0	37.0
7	35.31226364127499	37.0	35.0	37.0	32.0	37.0
8	35.32522960561858	37.0	35.0	37.0	32.0	37.0
9	36.7947055645597	39.0	37.0	39.0	32.0	39.0
10	36.92544570502431	39.0	37.0	39.0	33.0	39.0
11	36.89465153970826	39.0	37.0	39.0	32.0	39.0
12	36.80226904376013	39.0	37.0	39.0	32.0	39.0
13	36.95515937331172	39.0	37.0	39.0	33.0	39.0
14	38.067531064289575	40.0	37.0	41.0	33.0	41.0
15	38.04970286331712	40.0	37.0	41.0	33.0	41.0
16	38.00270124257158	40.0	37.0	41.0	33.0	41.0
17	37.968665586169635	40.0	37.0	41.0	33.0	41.0
18	38.05726634251756	40.0	37.0	41.0	33.0	41.0
19	38.197190707725554	40.0	37.0	41.0	33.0	41.0
20	38.2052944354403	40.0	37.0	41.0	34.0	41.0
21	38.07347379794705	40.0	37.0	41.0	33.0	41.0
22	37.96596434359805	40.0	37.0	41.0	33.0	41.0
23	37.69746083198271	40.0	37.0	41.0	32.0	41.0
24	37.727174500270124	39.0	37.0	41.0	32.0	41.0
25	37.76715289032955	39.0	37.0	41.0	32.0	41.0
26	37.58238789843328	39.0	37.0	41.0	32.0	41.0
27	37.56780118854673	39.0	37.0	41.0	32.0	41.0
28	37.37817396002161	39.0	36.0	41.0	32.0	41.0
29	37.27498649378714	39.0	36.0	41.0	31.0	41.0
30	37.05888708806051	39.0	36.0	41.0	31.0	41.0
31	37.00108049702863	39.0	36.0	40.0	30.0	41.0
32	36.874122096164236	39.0	35.0	40.0	30.0	41.0
33	36.81523500810373	39.0	36.0	41.0	30.0	41.0
34	36.69151809832523	39.0	35.0	40.0	30.0	41.0
35	36.40842787682334	39.0	35.0	40.0	29.0	41.0
36	36.40950837385197	39.0	35.0	40.0	30.0	41.0
37	36.57482441923285	39.0	35.0	40.0	30.0	41.0
38	36.621285791464075	39.0	35.0	40.0	30.0	41.0
39	36.450027012425714	39.0	35.0	40.0	30.0	41.0
40	36.11021069692058	39.0	35.0	40.0	29.0	41.0
41	35.75472717450027	39.0	35.0	40.0	26.0	41.0
42	35.48082117774176	39.0	35.0	40.0	25.0	41.0
43	34.66018368449487	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	2.0
24	9.0
25	4.0
26	14.0
27	14.0
28	34.0
29	28.0
30	41.0
31	55.0
32	65.0
33	80.0
34	102.0
35	112.0
36	187.0
37	254.0
38	431.0
39	418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.95191788222582	24.149108589951375	13.884386817936251	23.014586709886547
2	18.206374932468936	19.394921663965427	37.27714748784441	25.121555915721235
3	18.53052404105889	20.907617504051863	30.2539168017288	30.30794165316045
4	13.452188006482983	17.558076715289033	31.5505132360886	37.439222042139384
5	15.829281469475958	33.00918422474338	35.872501350621285	15.289032955159373
6	38.03349540788763	33.81955699621826	13.830361966504592	14.31658562938952
7	29.281469475958943	33.33333333333333	21.609940572663426	15.7752566180443
8	30.63209076175041	31.92868719611021	20.151269584008645	17.28795245813074
9	24.959481361426256	18.47649918962723	19.016747703943814	37.5472717450027
10	16.099405726634252	26.36412749864938	36.03457590491626	21.501890869800107
11	36.35872501350621	18.80064829821718	21.88006482982172	22.960561858454888
12	21.177741761210157	24.473257698541328	30.361966504592115	23.9870340356564
13	28.30902215018909	19.17882225823879	24.527282549972988	27.984873041599133
14	22.042139384116695	19.773095623987032	27.660723933009184	30.524041058887086
15	23.122636412749863	26.148028092922743	22.042139384116695	28.687196110210696
16	27.768773635872503	24.79740680713128	24.04105888708806	23.392760669908157
17	22.636412749864938	25.877903835764453	28.092922744462452	23.392760669908157
18	23.3387358184765	27.22852512155591	25.93192868719611	23.500810372771475
19	24.149108589951375	25.283630470016206	29.227444624527283	21.339816315505132
20	27.17450027012426	21.7720151269584	26.36412749864938	24.689357104267966
21	23.9870340356564	23.933009184224744	25.877903835764453	26.202052944354403
22	23.500810372771475	24.851431658562937	25.283630470016206	26.36412749864938
23	26.418152350081037	23.28471096704484	26.580226904376016	23.71690977849811
24	22.906537007023232	22.09616423554835	30.63209076175041	24.365207995678013
25	21.44786601836845	23.933009184224744	26.85035116153431	27.768773635872503
26	22.690437601296594	24.743381955699622	30.47001620745543	22.09616423554835
27	22.852512155591572	22.366288492706644	30.41599135602377	24.365207995678013
28	21.231766612641813	24.203133441383038	28.417071853052406	26.148028092922743
29	22.798487304159913	23.824959481361425	30.740140464613724	22.636412749864938
30	27.17450027012426	23.446785521339816	26.36412749864938	23.014586709886547
31	22.906537007023232	24.311183144246353	26.039978390059424	26.742301458670987
32	22.960561858454888	24.79740680713128	29.65964343598055	22.58238789843328
33	22.744462452728257	24.203133441383038	30.199891950297136	22.852512155591572
34	25.661804430037815	25.06753106428957	25.93192868719611	23.3387358184765
35	23.014586709886547	27.930848190167477	25.44570502431118	23.60886007563479
36	23.500810372771475	30.037817396002158	25.013506212857916	21.44786601836845
37	22.25823878984333	27.22852512155591	26.526202052944353	23.9870340356564
38	22.906537007023232	26.580226904376016	27.822798487304162	22.690437601296594
39	22.52836304700162	25.5537547271745	27.606699081577524	24.311183144246353
40	22.52836304700162	27.876823338735818	27.444624527282553	22.15018908698001
41	23.122636412749863	26.148028092922743	26.580226904376016	24.149108589951375
42	21.231766612641813	26.68827660723933	28.795245813074015	23.28471096704484
43	22.420313344138304	26.958400864397625	26.25607779578606	24.365207995678013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	3.0
9	3.0
10	4.0
11	5.0
12	5.0
13	5.0
14	5.0
15	7.5
16	10.0
17	15.5
18	21.0
19	21.0
20	20.0
21	19.0
22	15.5
23	12.0
24	16.0
25	20.0
26	20.0
27	22.5
28	25.0
29	33.0
30	41.0
31	45.5
32	50.0
33	50.0
34	60.5
35	71.0
36	72.5
37	74.0
38	105.0
39	136.0
40	136.0
41	137.0
42	138.0
43	127.5
44	117.0
45	116.5
46	116.0
47	116.0
48	120.5
49	125.0
50	123.0
51	121.0
52	126.0
53	131.0
54	131.0
55	123.0
56	115.0
57	114.0
58	113.0
59	108.5
60	104.0
61	104.0
62	97.0
63	90.0
64	79.0
65	68.0
66	53.5
67	39.0
68	39.0
69	34.5
70	30.0
71	27.5
72	25.0
73	17.0
74	9.0
75	9.0
76	7.5
77	6.0
78	5.0
79	4.0
80	2.0
81	0.0
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1851.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.73203673689898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.47629796839729	94.2733657482442
2	0.9029345372460496	1.728795245813074
3	0.33860045146726864	0.9724473257698543
4	0.0	0.0
5	0.0564334085778781	0.2701242571582928
6	0.1128668171557562	0.6482982171799028
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.1128668171557562	2.106969205834684
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	28	1.5126958400864399	TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.5942733657482442	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.3241491085899514	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.3241491085899514	No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAACA	5	0.2701242571582928	TruSeq Adapter, Index 25 (96% over 27bp)
AGATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3	0.1620745542949757	TruSeq Adapter, Index 25 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1620745542949757	No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAATA	3	0.1620745542949757	TruSeq Adapter, Index 25 (96% over 27bp)
GATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3	0.1620745542949757	TruSeq Adapter, Index 25 (96% over 27bp)
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC	3	0.1620745542949757	TruSeq Adapter, Index 25 (96% over 27bp)
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT	3	0.1620745542949757	TruSeq Adapter, Index 25 (96% over 27bp)
TCCTGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAA	2	0.10804970286331712	RNA PCR Primer, Index 40 (96% over 30bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	2	0.10804970286331712	No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTT	2	0.10804970286331712	No Hit
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCG	2	0.10804970286331712	No Hit
GAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCAC	2	0.10804970286331712	No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCG	2	0.10804970286331712	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	2	0.10804970286331712	No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAATGA	2	0.10804970286331712	TruSeq Adapter, Index 25 (96% over 27bp)
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA	2	0.10804970286331712	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2	0.10804970286331712	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	2	0.10804970286331712	No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAATT	2	0.10804970286331712	TruSeq Adapter, Index 25 (96% over 27bp)
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCG	2	0.10804970286331712	No Hit
GACCAGAGGAGGTCACACATGGGCCCATCATGAGGCACCTCTT	2	0.10804970286331712	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	2	0.10804970286331712	No Hit
TCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	2	0.10804970286331712	Illumina PCR Primer Index 6 (96% over 26bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.1620745542949757	0.0
30	0.0	0.0	0.0	0.2701242571582928	0.0
31	0.0	0.0	0.0	0.5942733657482442	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE