FastQCFastQC Report
Fri 10 Feb 2017
ERR1631992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79355
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3630.45743809463801904No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3000.3780480120975364No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2800.35284481129103396No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2580.32512129040388127No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2520.3175603301619306No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2360.29739776951672864No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2270.2860563291538025No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2160.27219456871022624No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1850.23312960746014744No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1750.2205280070568962No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1740.2192678470165711No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1680.21170688677462035No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1670.21044672673429526No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1660.20918656669397015No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1660.20918656669397015No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1560.19658496629071892No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1510.19028416608909332No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1360.1713817654842165No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1320.166341125322916No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1280.16130048516161552No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1230.15499968495998992No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1230.15499968495998992No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1230.15499968495998992No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1220.15373952491966478No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1200.15121920483901455No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1180.14869888475836432No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1140.14365824459706383No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1110.13987776447608846No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1100.13861760443576335No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1050.13231680423413775No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1040.1310566441938126No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1010.12727616407283726No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1010.12727616407283726No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1010.12727616407283726No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC990.12475584399218702No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT990.12475584399218702No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA980.12349568395186189No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA970.12223552391153678No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC970.12223552391153678No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA960.12097536387121165No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC960.12097536387121165No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT950.11971520383088652No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC940.1184550437905614No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG930.11719488375023628No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA890.11215424358893579No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC890.11215424358893579No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC880.11089408354861069No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC880.11089408354861069No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA860.10837376346796042No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA850.10711360342763532No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT840.10585344338731018No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC830.10459328334698507No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG820.10333312330665995No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG810.10207296326633483No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT800.1008128032260097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT251.2232973E-437.030
TCTAAGT200.001831629937.033
ATACGGC251.2232973E-437.029
CTCTATG451.0585609E-732.888891
GGTATCA1450.031.8965511
CTGCGGG352.3606053E-531.71428720
TGCGGGT352.3606053E-531.71428721
TCCGTGC1550.029.838718
TAAGTAC250.005465940629.635
CTAAGTA250.005465940629.634
TCTATGG502.6671842E-729.62
GCGGTAA1500.029.59999823
CGTGCCA1500.029.59999810
CCGTGCC1550.028.6451639
AACTCCG1550.028.6451635
AACGTCA405.871781E-527.7528
CAATGAG405.871781E-527.7533
GTCAATG405.871781E-527.7531
CGGGTAA405.871781E-527.7523
CTCCGTG1600.027.757
Produced by FastQC (version 0.11.5)