##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631992.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79355
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57339802154873	34.0	31.0	34.0	31.0	34.0
2	32.746065150274084	34.0	31.0	34.0	31.0	34.0
3	32.84014869888476	34.0	31.0	34.0	31.0	34.0
4	36.268703925398526	37.0	37.0	37.0	35.0	37.0
5	36.18967928926974	37.0	35.0	37.0	35.0	37.0
6	36.246499905487994	37.0	37.0	37.0	35.0	37.0
7	36.242416986957345	37.0	37.0	37.0	35.0	37.0
8	36.227610106483525	37.0	37.0	37.0	35.0	37.0
9	38.069598639027156	39.0	38.0	39.0	37.0	39.0
10	37.98972969567135	39.0	38.0	39.0	35.0	39.0
11	38.05011656480373	39.0	38.0	39.0	35.0	39.0
12	37.97899313212778	39.0	38.0	39.0	35.0	39.0
13	38.05412387373197	39.0	38.0	39.0	35.0	39.0
14	39.42413206477222	40.0	39.0	41.0	37.0	41.0
15	39.44348812299162	40.0	39.0	41.0	37.0	41.0
16	39.34995904479869	40.0	39.0	41.0	36.0	41.0
17	39.3174343141579	40.0	39.0	41.0	36.0	41.0
18	39.35622204019911	40.0	39.0	41.0	36.0	41.0
19	39.37874110011972	40.0	39.0	41.0	37.0	41.0
20	39.393535378993136	40.0	39.0	41.0	37.0	41.0
21	39.34471677903094	40.0	39.0	41.0	37.0	41.0
22	39.32554974481759	40.0	39.0	41.0	36.0	41.0
23	39.283548610673556	40.0	39.0	41.0	36.0	41.0
24	39.295784764665115	40.0	39.0	41.0	36.0	41.0
25	39.20976624031252	40.0	39.0	41.0	36.0	41.0
26	39.1616155251717	40.0	39.0	41.0	36.0	41.0
27	38.99872723835927	40.0	39.0	41.0	35.0	41.0
28	39.03189465062063	40.0	39.0	41.0	35.0	41.0
29	39.01325688362422	40.0	39.0	41.0	35.0	41.0
30	38.991191481318126	40.0	38.0	41.0	35.0	41.0
31	38.87966731774935	40.0	38.0	41.0	35.0	41.0
32	38.86864091739651	40.0	38.0	41.0	35.0	41.0
33	38.802810156889926	40.0	38.0	41.0	35.0	41.0
34	38.78175288261609	40.0	38.0	41.0	35.0	41.0
35	38.69981727679415	40.0	38.0	41.0	35.0	41.0
36	38.67012790624409	40.0	38.0	41.0	35.0	41.0
37	38.61039632033268	40.0	38.0	41.0	35.0	41.0
38	38.57530086320963	40.0	38.0	41.0	35.0	41.0
39	38.467153928548925	40.0	38.0	41.0	34.0	41.0
40	38.17065087266083	40.0	37.0	41.0	34.0	41.0
41	38.26607019091425	40.0	37.0	41.0	34.0	41.0
42	38.246071451074286	40.0	37.0	41.0	34.0	41.0
43	37.76596307731082	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	0.0
21	2.0
22	9.0
23	20.0
24	22.0
25	58.0
26	100.0
27	156.0
28	251.0
29	354.0
30	609.0
31	735.0
32	980.0
33	1381.0
34	1999.0
35	3015.0
36	4672.0
37	8357.0
38	19812.0
39	36821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98601222355239	17.257891752252537	12.698632726356246	28.057463297838826
2	21.44414340621259	19.732846071451075	31.903471740911094	26.91953878142524
3	20.47508033520257	20.40703169302501	30.066158402117072	29.051729569655343
4	14.121353411883309	17.058786465881166	37.43683447797871	31.38302564425682
5	15.840211706886775	33.52151723268855	34.799319513578226	15.83895154684645
6	30.75798626425556	36.65805557305778	16.38586100434755	16.19809715833911
7	28.71274651880789	27.976813055258017	20.825404826412957	22.48503559952114
8	25.392224812551195	30.55888097788419	21.65080965282591	22.398084556738706
9	27.336651754772856	12.62554344401739	18.77764476088463	41.260160040325125
10	19.358578539474514	22.364060235649927	29.49782622393044	28.779535000945124
11	38.709596118707076	19.976056959233823	19.25902589628883	22.055321025770272
12	21.644508852624284	24.681494549807827	26.992628063764094	26.681368533803795
13	34.95053871841724	18.180328901770526	20.19406464621007	26.67506773360217
14	22.868124251779975	20.50910465629135	23.673366517547727	32.94940457438095
15	29.911158717157075	23.775439480814065	20.880851868187257	25.432549933841596
16	24.287064457186062	24.705437590574004	23.362106987587424	27.64539096465251
17	26.438157646021043	24.63360846827547	21.145485476655534	27.782748409047947
18	25.339298090857536	21.683573813874364	23.54104971331359	29.436078381954506
19	27.011530464368978	22.623653203956902	24.02243084871779	26.342385482956338
20	30.028353600907316	20.190284166089093	23.13275786024825	26.64860437275534
21	26.64356373259404	22.23174343141579	22.49259655976309	28.63209627622708
22	28.39518618864596	23.289017705248565	20.88841282842921	27.427383277676263
23	28.044861697435575	22.45101127843236	22.536702161174468	26.967424862957596
24	27.530716400982925	21.706256694600214	22.491336399722766	28.271690504694096
25	27.96043097473379	22.35019847520635	23.428895469724655	26.260475080335205
26	28.029739776951672	23.21844874299036	22.589628882868123	26.162182597189844
27	26.807384537836302	21.89780102072963	25.012916640413334	26.281897801020733
28	25.404826412954446	23.743935479805938	22.428328397706508	28.422909709533112
29	27.233318631466197	24.512633104404262	22.482515279440488	25.771532984689056
30	26.208808518681874	21.563858610043475	24.856656795413016	27.37067607586163
31	27.41982231743431	22.93239241383656	23.316741226135722	26.331044042593412
32	25.21328208682503	22.19267847016571	23.18946506206288	29.40457438094638
33	25.13767248440552	22.141011908512382	25.445151534244847	27.27616407283725
34	26.392791884569338	22.083044546657426	24.610925587549616	26.913237981223613
35	24.907063197026023	25.17295696553462	24.610925587549616	25.309054249889734
36	26.191166278117322	22.883246172263878	25.206981286623403	25.7186062629954
37	26.721693655094196	22.979018335328586	24.536576145170436	25.76271186440678
38	25.524541616785335	21.894020540608658	25.65181778085817	26.929620061747844
39	24.79616911347741	21.222355239115366	26.809904857916955	27.17157078949026
40	24.544137105412386	22.573246802343895	27.34421271501481	25.538403377228907
41	23.197026022304833	21.18076995778464	27.625228404007306	27.996975615903217
42	20.864469787663033	22.372881355932204	29.44363934219646	27.319009514208304
43	20.66158402117069	22.696742486295758	28.61445403566253	28.027219456871023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.5
4	3.0
5	3.0
6	4.0
7	5.0
8	4.0
9	3.0
10	5.0
11	7.0
12	7.0
13	20.0
14	33.0
15	75.0
16	117.0
17	105.0
18	93.0
19	93.0
20	106.5
21	120.0
22	87.5
23	55.0
24	50.5
25	46.0
26	46.0
27	60.5
28	75.0
29	104.0
30	133.0
31	225.5
32	318.0
33	318.0
34	521.0
35	724.0
36	896.5
37	1069.0
38	1497.5
39	1926.0
40	1926.0
41	2322.0
42	2718.0
43	3445.5
44	4173.0
45	5538.0
46	6903.0
47	6903.0
48	7647.5
49	8392.0
50	9640.5
51	10889.0
52	10600.0
53	10311.0
54	10311.0
55	9239.5
56	8168.0
57	7488.0
58	6808.0
59	6240.0
60	5672.0
61	5672.0
62	5011.5
63	4351.0
64	3344.5
65	2338.0
66	1939.0
67	1540.0
68	1540.0
69	1285.0
70	1030.0
71	827.0
72	624.0
73	479.0
74	334.0
75	334.0
76	268.0
77	202.0
78	151.5
79	101.0
80	70.0
81	39.0
82	39.0
83	31.5
84	24.0
85	15.5
86	7.0
87	4.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	79355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.93686598197971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02692388593407	31.883309180265897
2	10.159560082935185	8.521202192678471
3	4.03858289011088	5.080965282590889
4	2.241653895850236	3.760317560330162
5	1.400282460410469	2.936172893957533
6	1.0126506205114336	2.5480436015373953
7	0.7422098019772229	2.1788167097221347
8	0.50482286126386	1.6936550941969628
9	0.4297004116710238	1.6218259718984311
>10	3.0529763514528683	24.469787663033205
>50	0.28847020643649146	8.233885703484342
>100	0.1021665314462574	7.07201814630458
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	363	0.45743809463801904	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	300	0.3780480120975364	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	280	0.35284481129103396	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	258	0.32512129040388127	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	252	0.3175603301619306	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	236	0.29739776951672864	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	227	0.2860563291538025	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	216	0.27219456871022624	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	185	0.23312960746014744	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	175	0.2205280070568962	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	174	0.2192678470165711	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	168	0.21170688677462035	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	167	0.21044672673429526	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	166	0.20918656669397015	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	166	0.20918656669397015	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	156	0.19658496629071892	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	151	0.19028416608909332	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	136	0.1713817654842165	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	132	0.166341125322916	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	128	0.16130048516161552	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	123	0.15499968495998992	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	123	0.15499968495998992	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	123	0.15499968495998992	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	122	0.15373952491966478	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	120	0.15121920483901455	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	118	0.14869888475836432	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	114	0.14365824459706383	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	111	0.13987776447608846	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	110	0.13861760443576335	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	105	0.13231680423413775	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	104	0.1310566441938126	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	101	0.12727616407283726	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	101	0.12727616407283726	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	101	0.12727616407283726	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	99	0.12475584399218702	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	99	0.12475584399218702	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	98	0.12349568395186189	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	97	0.12223552391153678	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	97	0.12223552391153678	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	96	0.12097536387121165	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	96	0.12097536387121165	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	95	0.11971520383088652	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	94	0.1184550437905614	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	93	0.11719488375023628	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	89	0.11215424358893579	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	89	0.11215424358893579	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	88	0.11089408354861069	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	88	0.11089408354861069	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	86	0.10837376346796042	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	85	0.10711360342763532	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	84	0.10585344338731018	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	83	0.10459328334698507	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	82	0.10333312330665995	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	81	0.10207296326633483	No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT	80	0.1008128032260097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0012601600403251212	0.0
19	0.0	0.0	0.0	0.0012601600403251212	0.0
20	0.0	0.0	0.0	0.0012601600403251212	0.0
21	0.0	0.0	0.0	0.0012601600403251212	0.0012601600403251212
22	0.0	0.0	0.0	0.003780480120975364	0.0012601600403251212
23	0.0	0.0	0.0	0.0063008002016256065	0.0012601600403251212
24	0.0	0.0	0.0	0.008821120282275849	0.0012601600403251212
25	0.0	0.0	0.0	0.01008128032260097	0.0012601600403251212
26	0.0	0.0	0.0	0.013861760443576334	0.0012601600403251212
27	0.0	0.0	0.0	0.025203200806502426	0.0012601600403251212
28	0.0	0.0	0.0	0.09955264318568458	0.0012601600403251212
29	0.0	0.0	0.0	0.2306092873794972	0.0012601600403251212
30	0.0	0.0	0.0	0.38686913237981224	0.0012601600403251212
31	0.0	0.0	0.0	0.820364186251654	0.0012601600403251212
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	25	1.2232973E-4	37.0	30
TCTAAGT	20	0.0018316299	37.0	33
ATACGGC	25	1.2232973E-4	37.0	29
CTCTATG	45	1.0585609E-7	32.88889	1
GGTATCA	145	0.0	31.896551	1
CTGCGGG	35	2.3606053E-5	31.714287	20
TGCGGGT	35	2.3606053E-5	31.714287	21
TCCGTGC	155	0.0	29.83871	8
TAAGTAC	25	0.0054659406	29.6	35
CTAAGTA	25	0.0054659406	29.6	34
TCTATGG	50	2.6671842E-7	29.6	2
GCGGTAA	150	0.0	29.599998	23
CGTGCCA	150	0.0	29.599998	10
CCGTGCC	155	0.0	28.645163	9
AACTCCG	155	0.0	28.645163	5
AACGTCA	40	5.871781E-5	27.75	28
CAATGAG	40	5.871781E-5	27.75	33
GTCAATG	40	5.871781E-5	27.75	31
CGGGTAA	40	5.871781E-5	27.75	23
CTCCGTG	160	0.0	27.75	7
>>END_MODULE