FastQCFastQC Report
Fri 10 Feb 2017
ERR1631991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53794
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1360.2528162992155259No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1120.2082016581774919No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1120.2082016581774919No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1110.20634271480090716No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA970.18031750752872067No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT890.16544596051604268No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC880.16358701713945792No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC870.16172807376287318No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT860.15986913038628842No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT810.15057441350336467No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG810.15057441350336467No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA740.13756180986727146No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT730.1357028664906867No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA710.1319849797375172No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC670.1245492062311782No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC600.11153660259508495No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA600.11153660259508495No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA590.1096776592185002No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT590.1096776592185002No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC560.10410082908874595No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC550.10224188571216122No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA550.10224188571216122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA507.059498E-933.3000031
CCTTGGC250.005450430329.62
CCCTTGG250.005450430329.61
TTATACA508.882462E-625.90000237
GGTTCAC451.3004337E-424.6666686
GTACTGG551.8593011E-523.5454541
TGGCCCC400.001907468423.1258
TTCACTA502.655961E-422.28
TGGTTCA502.655961E-422.25
TACTGGT502.655961E-422.22
TCACTAT502.655961E-422.29
ACTATCG502.655961E-422.211
GTATCAA806.709852E-720.81252
GCAGTCA450.003779265820.55555713
GTTCACT555.0559815E-420.1818187
CTGGTTC555.0559815E-420.1818184
CTTATAC1101.6480044E-920.18181837
CTATCGG555.0559815E-420.18181812
TAGTGGC500.00695011718.55
TATCGGT609.081636E-418.513
Produced by FastQC (version 0.11.5)