##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631991.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53794
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.532959066066844	34.0	31.0	34.0	31.0	34.0
2	32.70147228315425	34.0	31.0	34.0	31.0	34.0
3	32.79213295163029	34.0	31.0	34.0	31.0	34.0
4	36.246235639662416	37.0	37.0	37.0	35.0	37.0
5	36.162341525077146	37.0	35.0	37.0	35.0	37.0
6	36.20227162880619	37.0	36.0	37.0	35.0	37.0
7	36.18338476410008	37.0	36.0	37.0	35.0	37.0
8	36.16245306167974	37.0	36.0	37.0	35.0	37.0
9	37.993140498940406	39.0	38.0	39.0	35.0	39.0
10	37.89848310220471	39.0	38.0	39.0	35.0	39.0
11	37.99951667472209	39.0	38.0	39.0	35.0	39.0
12	37.911774547347285	39.0	38.0	39.0	35.0	39.0
13	37.99719299550136	39.0	38.0	39.0	35.0	39.0
14	39.332416254600886	40.0	39.0	41.0	36.0	41.0
15	39.34847752537458	40.0	39.0	41.0	36.0	41.0
16	39.27679666877347	40.0	39.0	41.0	36.0	41.0
17	39.262854593449084	40.0	39.0	41.0	36.0	41.0
18	39.2631706138231	40.0	39.0	41.0	36.0	41.0
19	39.28672342640443	40.0	39.0	41.0	36.0	41.0
20	39.28664906866937	40.0	39.0	41.0	36.0	41.0
21	39.243874781574156	40.0	39.0	41.0	36.0	41.0
22	39.21481949659813	40.0	39.0	41.0	36.0	41.0
23	39.152693608952674	40.0	39.0	41.0	36.0	41.0
24	39.169275383871806	40.0	39.0	41.0	36.0	41.0
25	39.109064207904225	40.0	39.0	41.0	36.0	41.0
26	39.06076885898055	40.0	39.0	41.0	36.0	41.0
27	38.928486448302785	40.0	38.0	41.0	35.0	41.0
28	38.92067888612113	40.0	38.0	41.0	35.0	41.0
29	38.89582481317619	40.0	38.0	41.0	35.0	41.0
30	38.85366397739525	40.0	38.0	41.0	35.0	41.0
31	38.77813510800461	40.0	38.0	41.0	35.0	41.0
32	38.723668067070676	40.0	38.0	41.0	35.0	41.0
33	38.67269583968472	40.0	38.0	41.0	35.0	41.0
34	38.64583410789307	40.0	38.0	41.0	35.0	41.0
35	38.56902256757259	40.0	38.0	41.0	34.0	41.0
36	38.512677993828305	40.0	38.0	41.0	34.0	41.0
37	38.46572108413578	40.0	38.0	41.0	34.0	41.0
38	38.42277949213667	40.0	38.0	41.0	34.0	41.0
39	38.344629512585044	40.0	38.0	41.0	34.0	41.0
40	38.04548834442503	40.0	37.0	41.0	34.0	41.0
41	38.10846934602372	40.0	37.0	41.0	34.0	41.0
42	38.096237498605795	40.0	37.0	41.0	34.0	41.0
43	37.61848905082351	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	3.0
21	6.0
22	9.0
23	20.0
24	33.0
25	51.0
26	95.0
27	119.0
28	182.0
29	262.0
30	381.0
31	531.0
32	753.0
33	1005.0
34	1470.0
35	2264.0
36	3335.0
37	5943.0
38	13577.0
39	23754.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.292820760679625	16.250882998103876	12.85459344908354	28.60170279213295
2	20.954009740863295	19.117373684797563	32.11510577387813	27.813510800461017
3	20.390749897758116	19.63230100011154	29.436368368219508	30.540580733910843
4	15.287950329032979	15.877235379410342	33.81046213332342	35.02435215823326
5	16.602223296278396	33.046436405547084	33.66360560657322	16.687734691601293
6	33.84578205747853	34.58935940811243	15.455255232925605	16.109603301483435
7	30.702308807673717	28.103505967208235	19.30884485258579	21.88534037253225
8	27.68524370747667	30.642822619623004	20.242034427631335	21.429899245268988
9	28.499460906420794	12.3842807748076	17.04279287652898	42.07346544224263
10	19.14153994869316	23.283265791723984	29.34156225601368	28.23363200356917
11	39.47652154515374	19.373907870766256	18.437000408967545	22.712570175112464
12	22.812953117448043	23.740565862363834	26.396995947503438	27.049485072684686
13	35.44819124809459	17.076253857307506	20.522734877495633	26.95282001710228
14	24.46741272260847	19.73826077257687	22.489496969922296	33.30482953489236
15	29.56835334795702	23.5007621667844	19.569096925307655	27.361787559950923
16	25.803993010372906	23.963639067554002	21.62508829981039	28.607279622262705
17	27.417555861248466	23.623452429638995	21.16407034241737	27.79492136669517
18	27.343198126185076	20.81087110086627	22.972822247834333	28.873108525114326
19	28.757854035766073	21.944826560582964	22.805517343941702	26.49180205970926
20	30.956984050265827	20.260623861397182	21.751496449418152	27.03089563891884
21	28.016135628508753	21.701304978250363	20.97631706138231	29.306242331858574
22	29.335985425883926	22.164181879019964	20.43350559541956	28.066327099676542
23	28.943748373424544	21.69758709149719	21.39457932111388	27.964085213964385
24	27.915752686173178	21.186377662936387	22.134438784994607	28.763430865895824
25	28.10908279733799	22.017325352269772	22.45975387589694	27.413837974495298
26	28.41023162434472	22.130720898241442	21.976428597984906	27.482618879428934
27	27.099676543852475	21.541435847864072	23.604862995873148	27.754024612410305
28	26.62006915269361	22.720005948618805	21.232851247351007	29.42707365133658
29	27.30230137190021	23.50819794029074	21.968992824478565	27.220507863330486
30	27.194482656058295	21.70502286500353	23.151280811986467	27.949213666951707
31	27.746588838903968	22.298025802134067	22.376101423950626	27.57928393501134
32	26.116295497639143	21.80540580733911	22.67910919433394	29.39918950068781
33	26.008476781797224	21.64553667695282	24.36702978027289	27.97895676097706
34	26.828270810871103	21.402015094620218	23.619734542885823	28.149979551622856
35	26.023348328809902	23.026731605755288	24.478566382867978	26.47135368256683
36	26.170204855560097	21.88905825928542	25.025095735583896	26.915641149570586
37	26.610774435810686	21.72361229876938	24.53061679741235	27.134996468007582
38	25.48053686284716	21.11759675800275	25.78540357660706	27.616462802543033
39	24.841060341302004	20.09889578763431	26.555006134513143	28.50503773655054
40	24.52689891065918	20.59151578242927	27.988251477859983	26.89333382905157
41	22.88359296575826	20.16209986243819	28.830352827452877	28.123954344350672
42	21.673420827601593	20.113767334646987	30.462505112094284	27.750306725657136
43	20.383314124251775	21.037662192809606	29.87322006171692	28.705803621221698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	5.0
9	7.0
10	8.5
11	10.0
12	10.0
13	17.0
14	24.0
15	40.5
16	57.0
17	57.5
18	58.0
19	58.0
20	60.5
21	63.0
22	66.5
23	70.0
24	74.0
25	78.0
26	78.0
27	107.5
28	137.0
29	179.0
30	221.0
31	294.5
32	368.0
33	368.0
34	455.0
35	542.0
36	640.0
37	738.0
38	931.0
39	1124.0
40	1124.0
41	1329.5
42	1535.0
43	1915.5
44	2296.0
45	3058.0
46	3820.0
47	3820.0
48	4335.0
49	4850.0
50	5402.5
51	5955.0
52	6188.0
53	6421.0
54	6421.0
55	5879.5
56	5338.0
57	5058.5
58	4779.0
59	4479.5
60	4180.0
61	4180.0
62	3890.0
63	3600.0
64	2948.5
65	2297.0
66	2044.0
67	1791.0
68	1791.0
69	1489.5
70	1188.0
71	1045.0
72	902.0
73	731.0
74	560.0
75	560.0
76	440.5
77	321.0
78	261.0
79	201.0
80	160.5
81	120.0
82	120.0
83	90.5
84	61.0
85	47.5
86	34.0
87	28.5
88	23.0
89	23.0
90	18.0
91	13.0
92	10.5
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	53794.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.60430531286016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.73065980219482	60.187009703684424
2	5.9477035632028175	8.160761423207049
3	2.075599512261211	4.271851879391754
4	1.230185611705731	3.3758411718779047
5	0.7451564828614009	2.5560471428040303
6	0.4579325294675518	1.8849685838569359
7	0.28180463351849344	1.3533107781536975
8	0.26825633383010433	1.4722831542551214
9	0.2411597344533261	1.4890136446443842
>10	0.9592196179379487	11.865635572740455
>50	0.05148353881587861	2.5077146150128264
>100	0.010838639750711285	0.8755623303714168
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	136	0.2528162992155259	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	112	0.2082016581774919	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	112	0.2082016581774919	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	111	0.20634271480090716	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	97	0.18031750752872067	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	89	0.16544596051604268	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	88	0.16358701713945792	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	87	0.16172807376287318	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	86	0.15986913038628842	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	81	0.15057441350336467	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	81	0.15057441350336467	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	74	0.13756180986727146	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	73	0.1357028664906867	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	71	0.1319849797375172	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	67	0.1245492062311782	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	60	0.11153660259508495	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	60	0.11153660259508495	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	59	0.1096776592185002	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	59	0.1096776592185002	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	56	0.10410082908874595	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	55	0.10224188571216122	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	55	0.10224188571216122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0018589433765847493	0.0
20	0.0	0.0	0.0	0.0037178867531694987	0.0
21	0.0	0.0	0.0	0.009294716882923746	0.0
22	0.0	0.0	0.0	0.014871547012677995	0.0
23	0.0	0.0	0.0	0.014871547012677995	0.0
24	0.0	0.0	0.0	0.016730490389262744	0.0
25	0.0	0.0	0.0	0.016730490389262744	0.0
26	0.0	0.0	0.0	0.018589433765847492	0.0
27	0.0	0.0	0.0	0.04461464103803398	0.0
28	0.0	0.0	0.0	0.16544596051604268	0.0
29	0.0	0.0	0.0	0.3662118451871956	0.0
30	0.0	0.0	0.0	0.5948618805071197	0.0
31	0.0	0.0	0.0	1.2547867791947058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	7.059498E-9	33.300003	1
CCTTGGC	25	0.0054504303	29.6	2
CCCTTGG	25	0.0054504303	29.6	1
TTATACA	50	8.882462E-6	25.900002	37
GGTTCAC	45	1.3004337E-4	24.666668	6
GTACTGG	55	1.8593011E-5	23.545454	1
TGGCCCC	40	0.0019074684	23.125	8
TTCACTA	50	2.655961E-4	22.2	8
TGGTTCA	50	2.655961E-4	22.2	5
TACTGGT	50	2.655961E-4	22.2	2
TCACTAT	50	2.655961E-4	22.2	9
ACTATCG	50	2.655961E-4	22.2	11
GTATCAA	80	6.709852E-7	20.8125	2
GCAGTCA	45	0.0037792658	20.555557	13
GTTCACT	55	5.0559815E-4	20.181818	7
CTGGTTC	55	5.0559815E-4	20.181818	4
CTTATAC	110	1.6480044E-9	20.181818	37
CTATCGG	55	5.0559815E-4	20.181818	12
TAGTGGC	50	0.006950117	18.5	5
TATCGGT	60	9.081636E-4	18.5	13
>>END_MODULE