FastQCFastQC Report
Fri 10 Feb 2017
ERR1631990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126409
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6070.48018732843389317No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5640.4461707631576866No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5630.4453796802442864No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5520.43667776819688475No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5140.40661661748767886No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4950.39158604213307596No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4410.3488675648094677No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4340.3433299844156666No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4170.329881574887864No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3750.29665609252505754No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3720.2942828437848571No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3530.2792522684302542No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3400.26896819055605214No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3310.2618484443354508No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3200.2531465322880491No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3030.2396981227602465No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2750.2175478011850422No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2720.21517455244484174No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2710.21438346953144158No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2630.20805480622424039No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2610.20647264039744007No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2560.2025172258304393No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2550.20172614291703914No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2550.20172614291703914No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2460.19460639669643776No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2430.19223314795623728No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2420.19144206504283715No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2410.19065098212943699No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2400.18985989921603683No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2370.18748665047583637No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2330.18432231882223576No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2260.1787847384284347No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2250.17799365551503454No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2210.1748293238614339No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2210.1748293238614339No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2200.17403824094803377No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2150.170082826381033No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2120.16770957764083255No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2110.1669184947274324No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2110.1669184947274324No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2080.16454524598723191No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2070.16375416307383175No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2060.16296308016043162No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2020.159798748506831No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2010.15900766559343085No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1990.15742549976663053No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1950.15426116811302992No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1920.15188791937282947No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1860.14714142189242854No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1860.14714142189242854No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1830.1447681731522281No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1790.14160384149862745No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1770.14002167567182716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.13923059275842703No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1740.1376484269316267No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1730.13685734401822655No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1730.13685734401822655No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1700.13448409527802607No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1690.13369301236462594No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1670.13211084653782562No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1660.13131976362442546No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1640.12973759779762517No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1620.12815543197082488No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1560.12340893449042394No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG1560.12340893449042394No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG1540.12182676866362363No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1500.11866243701002302No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1480.11708027118322271No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1480.11708027118322271No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1480.11708027118322271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1470.11628918826982255No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1440.11391593952962209No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1440.11391593952962209No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC1430.11312485661622194No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1400.1107516078760215No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1380.10916944204922119No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG1380.10916944204922119No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1370.10837835913582103No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1350.10679619330902072No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1350.10679619330902072No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1340.10600511039562056No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1330.1052140274822204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1310.1036318616554201No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA1310.1036318616554201No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1290.10204969582861981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC200.001835746237.029
GGTATCA2850.030.5087741
AGTACCT250.00547808429.59999837
GGAACCC250.00547808429.59999831
CAGGAAC250.00547808429.59999829
TCCCCGG250.00547808429.59999810
GGGCTCA250.00547808429.59999815
ACATCTA1000.025.89999830
TAAGTCT451.314132E-424.66666835
CTAAGTC451.314132E-424.66666834
AAGTCTG451.314132E-424.66666836
AAACATC1051.8189894E-1224.66666628
TGAAACA1051.8189894E-1224.66666626
CTGAAAC1150.024.13043625
ATCCACC551.8853489E-523.54545428
GAAACAT1103.6379788E-1223.54545427
TCTAAGT1103.6379788E-1223.54545433
CCGTGTA400.00192174523.12533
TGCCGGA400.00192174523.12528
CCACCGT400.00192174523.12530
Produced by FastQC (version 0.11.5)