FastQCFastQC Report
Fri 10 Feb 2017
ERR1631989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496172
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11020.22210040066751044No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10870.21907725546786194No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10770.21706182533476293No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9840.1983183250969422No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9350.18844271744475707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.18400877115193925No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8620.17373007747313432No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8410.1694976741936264No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8270.16667607200728782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8230.1658698999540482No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7680.15478503422200365No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7570.15256806107559476No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7520.15156034600904525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7360.14833565779608682No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7320.14752948574284724No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7210.14531251259643832No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5970.12032117894601065No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5750.11588723265319285No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5660.11407334553340373No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5650.11387180252009382No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5530.11145328636037502No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5370.10822859814741662No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5340.10762396910748694No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5230.10540699596107801No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5110.10298847980135921No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5020.10117459268157011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT200.001840927837.029
TCACGTA200.001840927837.025
GTGTAAA250.00549337129.5999981
TCGTATG250.00549337129.59999830
ATGCTAG405.9334292E-527.75000215
AAACACG405.9334292E-527.75000215
ACGAAAG405.9334292E-527.75000219
GGTATCA7200.026.4652791
CACGTAG358.8622613E-426.42857226
ACGTAGG451.3217315E-424.66666827
GATGCTA551.8998346E-523.54545414
TTAGCTA400.001929648623.1250024
GCGGTAA4950.022.7979823
TATACAC1400.022.46428737
CGAAAGT502.6991044E-422.19999920
TTTACCG502.6991044E-422.19999930
CGCGGTA5200.022.05769222
GACCGTT603.720794E-521.5833347
TTACCGG603.720794E-521.58333431
GCAGTCG1301.8189894E-1221.3461539
Produced by FastQC (version 0.11.5)