##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631989.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	496172
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17287956595697	33.0	31.0	34.0	30.0	34.0
2	32.35687825995824	34.0	31.0	34.0	31.0	34.0
3	32.438297606475174	34.0	31.0	34.0	30.0	34.0
4	36.02208105253823	37.0	35.0	37.0	35.0	37.0
5	35.85064856541683	37.0	35.0	37.0	35.0	37.0
6	35.882837000072556	37.0	35.0	37.0	35.0	37.0
7	35.887986424062625	37.0	35.0	37.0	35.0	37.0
8	35.86248921744879	37.0	35.0	37.0	35.0	37.0
9	37.63711978910539	39.0	37.0	39.0	35.0	39.0
10	37.50986956136179	39.0	37.0	39.0	35.0	39.0
11	37.59401981570907	39.0	37.0	39.0	35.0	39.0
12	37.520194609933654	39.0	37.0	39.0	35.0	39.0
13	37.58853179945664	39.0	37.0	39.0	35.0	39.0
14	38.83379956950412	40.0	38.0	41.0	36.0	41.0
15	38.81759349580388	40.0	38.0	41.0	36.0	41.0
16	38.754053029997664	40.0	38.0	41.0	35.0	41.0
17	38.72165095974783	40.0	38.0	41.0	35.0	41.0
18	38.758204816071846	40.0	38.0	41.0	35.0	41.0
19	38.771230541022064	40.0	38.0	41.0	35.0	41.0
20	38.77160944188709	40.0	38.0	41.0	35.0	41.0
21	38.71826906798449	40.0	38.0	41.0	35.0	41.0
22	38.69175406915344	40.0	38.0	41.0	35.0	41.0
23	38.627659360060626	40.0	38.0	41.0	35.0	41.0
24	38.639294438219004	40.0	38.0	41.0	35.0	41.0
25	38.56799456640036	40.0	38.0	41.0	35.0	41.0
26	38.48150842852882	40.0	38.0	41.0	34.0	41.0
27	38.34566843755795	40.0	38.0	41.0	34.0	41.0
28	38.354469820949184	40.0	38.0	41.0	34.0	41.0
29	38.3156224051337	40.0	38.0	41.0	34.0	41.0
30	38.26761284393315	40.0	38.0	41.0	34.0	41.0
31	38.1699954854365	40.0	38.0	41.0	34.0	41.0
32	38.118156606983064	40.0	38.0	41.0	34.0	41.0
33	38.04550035068484	40.0	37.0	41.0	33.0	41.0
34	38.017165418443604	40.0	37.0	41.0	33.0	41.0
35	37.92980256846416	40.0	37.0	41.0	33.0	41.0
36	37.87921728755351	40.0	37.0	41.0	33.0	41.0
37	37.819498077279654	40.0	37.0	41.0	33.0	41.0
38	37.77678909732915	40.0	37.0	41.0	33.0	41.0
39	37.70394137516829	40.0	37.0	41.0	33.0	41.0
40	37.383433567391954	39.0	36.0	41.0	32.0	41.0
41	37.459058552276225	39.0	36.0	41.0	33.0	41.0
42	37.43583475085253	39.0	36.0	41.0	33.0	41.0
43	36.91883056681957	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	0.0
16	2.0
17	2.0
18	5.0
19	14.0
20	44.0
21	87.0
22	119.0
23	232.0
24	458.0
25	665.0
26	1128.0
27	1694.0
28	2635.0
29	3540.0
30	5107.0
31	6896.0
32	9269.0
33	12719.0
34	18002.0
35	26611.0
36	41593.0
37	75380.0
38	154035.0
39	135932.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.60115443838024	16.822593777964094	13.136170521512703	27.440081262142968
2	20.53642688422563	20.041034157509895	33.18224325435534	26.240295703909126
3	21.13037414444991	20.39514523189539	29.90676620204284	28.56771442161186
4	15.571011665309612	16.572277355433197	34.42999604975694	33.42671492950026
5	16.339696718073572	33.40152205283652	33.73568036890433	16.52310086018558
6	32.83357384132922	34.51403948630717	16.152261715695364	16.50012495666825
7	29.518392815394662	28.528211991003122	20.135154744725618	21.8182404488766
8	26.634312294929984	31.444942479624	20.579154003047332	21.341591222398684
9	28.014680393089492	13.246616092806526	17.948413050313196	40.79029046379078
10	19.05689962351765	23.89957514732794	29.78624348008352	27.257281749070888
11	38.420346170279664	20.102907862596034	19.451319300565125	22.025426666559177
12	22.1602589424635	24.43527647670566	27.097055053489516	26.307409527341324
13	34.510613255080905	18.12778633215901	21.506453407286184	25.85514700547391
14	23.551107277315126	20.488661190071188	23.998129680836485	31.96210185177721
15	29.261828559451157	24.606789581032384	20.655337262078472	25.476044597437987
16	24.594898543247098	24.701716340301346	23.15890457341406	27.544480543037498
17	26.267907096732586	24.406858911828962	22.212055496884144	27.113178494554308
18	25.983529904952313	21.990559725256563	24.013648492861346	28.012261876929774
19	27.79963399788783	22.854574623316108	24.02453181558008	25.321259563215982
20	29.41197810436703	21.177938295591044	23.588795820804076	25.821287779237846
21	26.71230944108092	22.887023048459003	22.58531315753408	27.815354352926004
22	27.51062131680143	23.425545980023056	22.0401393065308	27.02369339664471
23	27.184524721266012	22.808421273268138	23.09239537902179	26.914658626444055
24	27.330643405915687	22.135872237853	23.668203768048176	26.865280588183133
25	27.359464056819004	22.462775005441664	24.013245406834727	26.164515530904602
26	27.260506437283844	23.244963440097386	23.244761897084075	26.24976822553469
27	26.557524406858914	22.304160653966772	24.801480131889747	26.33683480728457
28	25.75860790209847	23.60330691776239	22.976508146368598	27.661577033770545
29	26.63995549930266	24.14021750521996	22.961795506396975	26.258031489080402
30	26.29834009174238	22.957160017090846	24.37118579847311	26.37331409269366
31	27.280459195601527	22.841675870464275	23.589601992857315	26.288262941076884
32	24.887337455559766	22.876341268753578	23.86309586191885	28.373225413767805
33	25.388978015688107	22.36482510097305	25.254548825810403	26.99164805752844
34	25.649774674911118	22.558306393750556	24.75472215280185	27.037196778536476
35	25.231774465306383	23.80404375901905	25.22351120176068	25.74067057391389
36	25.504260619301373	22.7933055472699	25.724950218875716	25.977483614553016
37	26.282619736704206	22.084277226445668	25.088275839829738	26.54482719702039
38	25.10863168417404	21.805946325064696	26.297533919689144	26.787888071072132
39	24.63500560289577	20.793797312222377	27.382843046362954	27.188354038518902
40	24.40907588497537	21.739235587659117	27.930233870512645	25.921454656852866
41	22.64658223358029	21.165039542739212	28.95911095345969	27.22926727022081
42	21.57276105866514	21.279919060325856	29.755407399047108	27.3919124819619
43	20.74179921478842	22.245310094080278	29.263642446570948	27.749248244560356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	9.5
2	14.0
3	22.5
4	31.0
5	31.0
6	47.0
7	63.0
8	67.0
9	71.0
10	122.5
11	174.0
12	174.0
13	322.0
14	470.0
15	1060.0
16	1650.0
17	1634.5
18	1619.0
19	1619.0
20	1749.0
21	1879.0
22	1585.5
23	1292.0
24	1350.0
25	1408.0
26	1408.0
27	1609.0
28	1810.0
29	2567.0
30	3324.0
31	3799.0
32	4274.0
33	4274.0
34	5506.0
35	6738.0
36	7355.0
37	7972.0
38	10611.5
39	13251.0
40	13251.0
41	15807.5
42	18364.0
43	20643.5
44	22923.0
45	31502.5
46	40082.0
47	40082.0
48	44606.5
49	49131.0
50	54615.0
51	60099.0
52	60659.0
53	61219.0
54	61219.0
55	53945.5
56	46672.0
57	43179.5
58	39687.0
59	36204.5
60	32722.0
61	32722.0
62	29432.5
63	26143.0
64	21433.0
65	16723.0
66	14506.5
67	12290.0
68	12290.0
69	10516.0
70	8742.0
71	7379.0
72	6016.0
73	4863.5
74	3711.0
75	3711.0
76	3011.0
77	2311.0
78	1923.5
79	1536.0
80	1178.0
81	820.0
82	820.0
83	636.0
84	452.0
85	357.0
86	262.0
87	200.0
88	138.0
89	138.0
90	97.5
91	57.0
92	40.5
93	24.0
94	13.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	496172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.348232172912965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06466631145562	39.889451360876265
2	6.218568351526586	5.764392994793657
3	2.1253193423776424	2.9551438296630503
4	1.1644905591105579	2.1588831518728546
5	0.7398498643119313	1.7145366642113773
6	0.4953328954378352	1.3774682424380407
7	0.39751112565210983	1.2896756560127978
8	0.29116170734308017	1.0795864329439055
9	0.21959533980002754	0.9160070213827254
>10	1.87930486061433	17.809019450477997
>50	0.24654864426289635	7.926709085393241
>100	0.14626509268852003	13.185607851249118
>500	0.010072147101305272	3.2706250371748706
>1k	0.001313758317561557	0.6628932215100612
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1102	0.22210040066751044	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1087	0.21907725546786194	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1077	0.21706182533476293	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	984	0.1983183250969422	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	935	0.18844271744475707	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	913	0.18400877115193925	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	862	0.17373007747313432	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	841	0.1694976741936264	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	827	0.16667607200728782	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.1658698999540482	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	768	0.15478503422200365	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	757	0.15256806107559476	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	752	0.15156034600904525	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	736	0.14833565779608682	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	732	0.14752948574284724	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	721	0.14531251259643832	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	597	0.12032117894601065	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	575	0.11588723265319285	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	566	0.11407334553340373	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	565	0.11387180252009382	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	553	0.11145328636037502	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	537	0.10822859814741662	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	534	0.10762396910748694	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	523	0.10540699596107801	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	511	0.10298847980135921	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	502	0.10117459268157011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.015430133099006E-4	0.0	0.0	0.0	0.0
9	2.015430133099006E-4	0.0	0.0	0.0	0.0
10	2.015430133099006E-4	0.0	0.0	2.015430133099006E-4	0.0
11	4.030860266198012E-4	0.0	0.0	2.015430133099006E-4	0.0
12	4.030860266198012E-4	0.0	0.0	2.015430133099006E-4	0.0
13	4.030860266198012E-4	0.0	0.0	4.030860266198012E-4	0.0
14	4.030860266198012E-4	0.0	0.0	6.046290399297018E-4	0.0
15	4.030860266198012E-4	0.0	0.0	0.0012092580798594035	0.0
16	4.030860266198012E-4	0.0	0.0	0.0022169731464089064	0.0
17	4.030860266198012E-4	0.0	0.0	0.0028216021863386085	0.0
18	0.001007715066549503	0.0	0.0	0.004030860266198012	0.0
19	0.001007715066549503	0.0	0.0	0.004837032319437614	0.0
20	0.001007715066549503	0.0	0.0	0.0070540054658465206	0.0
21	0.001007715066549503	0.0	0.0	0.011084865732044534	0.0
22	0.001007715066549503	0.0	0.0	0.014712639971622744	0.0
23	0.001007715066549503	0.0	0.0	0.01592189805148215	0.0
24	0.001007715066549503	0.0	0.0	0.01995275831768016	0.0
25	0.001007715066549503	0.0	0.0	0.020960473384229662	0.0
26	0.001007715066549503	0.0	0.0	0.025999048716977176	0.0
27	0.001007715066549503	0.0	0.0	0.04615335004796724	0.0
28	0.001007715066549503	0.0	0.0	0.14007239425038093	0.0
29	0.001007715066549503	0.0	0.0	0.2956636005256242	0.0
30	0.001007715066549503	0.0	0.0	0.5038575332747515	0.0
31	0.001007715066549503	0.0	0.0	1.1606862136517175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	20	0.0018409278	37.0	29
TCACGTA	20	0.0018409278	37.0	25
GTGTAAA	25	0.005493371	29.599998	1
TCGTATG	25	0.005493371	29.599998	30
ATGCTAG	40	5.9334292E-5	27.750002	15
AAACACG	40	5.9334292E-5	27.750002	15
ACGAAAG	40	5.9334292E-5	27.750002	19
GGTATCA	720	0.0	26.465279	1
CACGTAG	35	8.8622613E-4	26.428572	26
ACGTAGG	45	1.3217315E-4	24.666668	27
GATGCTA	55	1.8998346E-5	23.545454	14
TTAGCTA	40	0.0019296486	23.125002	4
GCGGTAA	495	0.0	22.79798	23
TATACAC	140	0.0	22.464287	37
CGAAAGT	50	2.6991044E-4	22.199999	20
TTTACCG	50	2.6991044E-4	22.199999	30
CGCGGTA	520	0.0	22.057692	22
GACCGTT	60	3.720794E-5	21.583334	7
TTACCGG	60	3.720794E-5	21.583334	31
GCAGTCG	130	1.8189894E-12	21.346153	9
>>END_MODULE