FastQCFastQC Report
Fri 10 Feb 2017
ERR1631988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430042
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.1613938611593226No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22620.15817717241871218No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA21910.1532122832755961No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA21670.1515340108891907No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC20620.14419156919866688No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20340.14223358474786055No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20050.14020567228095399No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC19390.13559042321833903No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19360.13538063917003837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19200.13426179091243473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18750.13111503018792453No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18070.12635992509310914No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18060.12628999707700894No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG17190.12020625967628921No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG16760.11719935498397949No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16600.11608050672637586No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16370.11447216235607066No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT15930.11139532964766069No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA15770.11027648139005708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG250.00549678629.69
GGTATCA15850.028.012621
TTACGAG601.3377885E-624.6666686
GATCTAA451.3234332E-424.6666661
GTACAAT551.9030804E-523.5454541
GTATAGA753.7420978E-722.21
CTTATAC14750.021.5728837
TACGAGG705.1033603E-621.1428597
GTAACGT5800.019.45689626
TGCGGGT5900.019.44067821
TTCTGCG5900.019.1271218
GCGGTAA8900.019.12359423
GCTTAGG5550.019.01
TAGGACA6050.018.6528934
ATAGACT500.007035700618.54
TCTAGCC701.2194613E-418.523
GTGCTAT902.152965E-618.51
ATAAGAG1306.9667294E-1018.514
AGTTAGA801.61712E-518.526
ATTATAC500.007035700618.53
Produced by FastQC (version 0.11.5)