##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631988.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1430042
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65590031621449	34.0	31.0	34.0	31.0	34.0
2	32.82023604901115	34.0	31.0	34.0	31.0	34.0
3	32.905704168129326	34.0	31.0	34.0	31.0	34.0
4	36.33272938836762	37.0	37.0	37.0	35.0	37.0
5	36.25234084033895	37.0	37.0	37.0	35.0	37.0
6	36.303014876486145	37.0	37.0	37.0	35.0	37.0
7	36.28859781740676	37.0	37.0	37.0	35.0	37.0
8	36.26916831813331	37.0	37.0	37.0	35.0	37.0
9	38.10047816777409	39.0	38.0	39.0	37.0	39.0
10	38.045886064884805	39.0	38.0	39.0	35.0	39.0
11	38.10840310983873	39.0	38.0	39.0	37.0	39.0
12	38.05670882393664	39.0	38.0	39.0	37.0	39.0
13	38.09649716581751	39.0	38.0	39.0	37.0	39.0
14	39.52266646713873	41.0	39.0	41.0	37.0	41.0
15	39.52040919077901	41.0	39.0	41.0	37.0	41.0
16	39.47527834846809	41.0	39.0	41.0	37.0	41.0
17	39.448189633591184	41.0	39.0	41.0	37.0	41.0
18	39.46408986589205	41.0	39.0	41.0	37.0	41.0
19	39.497566505039714	41.0	39.0	41.0	37.0	41.0
20	39.4874444247092	41.0	39.0	41.0	37.0	41.0
21	39.456815953657305	41.0	39.0	41.0	37.0	41.0
22	39.446237942661824	41.0	39.0	41.0	37.0	41.0
23	39.38146222278786	41.0	39.0	41.0	37.0	41.0
24	39.4059629017889	41.0	39.0	41.0	37.0	41.0
25	39.34904639164444	41.0	39.0	41.0	36.0	41.0
26	39.29831151812324	40.0	39.0	41.0	36.0	41.0
27	39.196615204308685	40.0	39.0	41.0	36.0	41.0
28	39.19771097632098	40.0	39.0	41.0	36.0	41.0
29	39.18216597834189	40.0	39.0	41.0	36.0	41.0
30	39.1403734995196	40.0	39.0	41.0	36.0	41.0
31	39.054651541702974	40.0	39.0	41.0	35.0	41.0
32	39.01015844289888	40.0	39.0	41.0	35.0	41.0
33	38.96074171248117	40.0	38.0	41.0	35.0	41.0
34	38.93231457537611	40.0	38.0	41.0	35.0	41.0
35	38.86324947099456	40.0	38.0	41.0	35.0	41.0
36	38.81898993176424	40.0	38.0	41.0	35.0	41.0
37	38.77689606319255	40.0	38.0	41.0	35.0	41.0
38	38.72721920055495	40.0	38.0	41.0	35.0	41.0
39	38.67368580782942	40.0	38.0	41.0	35.0	41.0
40	38.36669062866685	40.0	38.0	41.0	34.0	41.0
41	38.45343283623838	40.0	38.0	41.0	34.0	41.0
42	38.44081013005212	40.0	38.0	41.0	34.0	41.0
43	37.99058559119243	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	7.0
19	23.0
20	27.0
21	80.0
22	172.0
23	317.0
24	551.0
25	1005.0
26	1599.0
27	2519.0
28	4005.0
29	5936.0
30	8576.0
31	12071.0
32	16604.0
33	22417.0
34	33310.0
35	49897.0
36	77606.0
37	141195.0
38	327620.0
39	724498.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.34470036544381	17.141035018551904	13.191570597227214	27.32269401877707
2	18.998532910222217	20.238706275759732	35.09945861729935	25.663302196718696
3	20.059970266607554	21.038333139865824	30.479734161653994	28.421962431872632
4	14.835718111775739	16.236935698392074	34.47472172145993	34.45262446837226
5	15.532830504278897	34.652968234499404	34.18521973480499	15.628981526416707
6	33.353076343212294	34.70856100729909	15.989810089493876	15.948552559994742
7	29.421443565993165	29.50318941681433	20.568906367785004	20.506460649407497
8	26.299087719101955	33.23601684426052	20.412617251800995	20.05227818483653
9	27.49282888194892	14.27783239932813	18.272400391037465	39.956938327685485
10	18.173312392223444	25.337927137804346	30.7906341212356	25.69812634873661
11	37.234640660903665	20.79722134035224	20.452825861058628	21.515312137685466
12	22.098092223864754	24.84843102510276	27.489891905272714	25.563584845759774
13	32.57834385283789	18.71805163764421	22.997016870833164	25.706587638684734
14	23.156033179445078	20.48198584377242	24.18789098502002	32.17408999176248
15	27.781911300507257	25.798333195808237	21.595100004055826	24.82465549962868
16	25.021153224870318	25.366597624405436	22.941633882081785	26.67061526864246
17	25.73868459807474	25.093528721534053	22.8844327649118	26.283353915479406
18	24.96535066802234	23.293581587114225	24.840459231267335	26.900608513596104
19	27.169062167404874	23.716925796584995	24.632493311385257	24.48151872462487
20	27.94554285818179	22.38067133692577	24.704519167968495	24.96926663692395
21	25.694350235867198	24.363410305431586	23.20288495023223	26.73935450846898
22	26.38139299405192	24.20900924588229	22.837511066108547	26.572086693957242
23	25.67344175905323	24.310824437324218	24.06369882842602	25.95203497519653
24	27.049974755986188	23.401690300005175	23.795454958665548	25.752879985343085
25	26.638518309252458	23.450080487146533	24.483616565107877	25.427784638493133
26	26.67068519665856	23.849579243127124	23.720282341357805	25.75945321885651
27	25.97895726139512	23.161207852636494	24.79262846825478	26.067206417713606
28	24.88248596894357	24.27739884562831	23.964540901595896	26.875574283832222
29	25.961964753482764	24.901856029403334	23.552245318668962	25.58393389844494
30	25.187581903188853	24.50599353025995	24.535782865118648	25.770641701432545
31	26.298318510924858	23.735316864819357	24.411241068444145	25.555123555811647
32	24.013770224930457	23.983141753878556	24.5209581257054	27.482129895485585
33	24.724168940492657	23.236869966056943	25.63554077432691	26.403420319123494
34	24.898849124711024	23.40686497319659	25.34471015536607	26.349575746726323
35	24.896821212244117	24.35753635207917	25.144296461222815	25.6013459744539
36	24.99646863518694	23.676227691214663	25.85833143362223	25.468972239976168
37	25.93287469878507	22.68716583149306	25.226322024108384	26.153637445613487
38	24.707456144644702	22.50773054217988	26.564744252266713	26.220069060908703
39	24.339355067893113	21.59607899628123	27.409055118660852	26.655510817164814
40	23.971463775189818	22.302491814925716	28.20623450220343	25.519809907681037
41	22.546750375163807	22.011451411916575	29.070754565250528	26.37104364766909
42	22.01361918041568	21.90606989165353	29.225854904960833	26.854456022969952
43	20.776802359650976	22.632552050918783	29.505007545232935	27.085638044197303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	12.5
2	13.0
3	47.0
4	81.0
5	81.0
6	115.0
7	149.0
8	166.5
9	184.0
10	351.0
11	518.0
12	518.0
13	961.5
14	1405.0
15	3026.5
16	4648.0
17	4670.0
18	4692.0
19	4692.0
20	4996.5
21	5301.0
22	5252.0
23	5203.0
24	5688.5
25	6174.0
26	6174.0
27	7051.5
28	7929.0
29	12333.0
30	16737.0
31	18521.0
32	20305.0
33	20305.0
34	24765.0
35	29225.0
36	31081.0
37	32937.0
38	43855.5
39	54774.0
40	54774.0
41	63677.5
42	72581.0
43	74845.0
44	77109.0
45	95789.0
46	114469.0
47	114469.0
48	125076.0
49	135683.0
50	150140.0
51	164597.0
52	168376.0
53	172155.0
54	172155.0
55	146575.0
56	120995.0
57	109987.0
58	98979.0
59	88838.0
60	78697.0
61	78697.0
62	71205.5
63	63714.0
64	53352.0
65	42990.0
66	37291.5
67	31593.0
68	31593.0
69	27046.0
70	22499.0
71	19578.5
72	16658.0
73	13550.0
74	10442.0
75	10442.0
76	8492.5
77	6543.0
78	5291.0
79	4039.0
80	3244.5
81	2450.0
82	2450.0
83	2039.0
84	1628.0
85	1304.5
86	981.0
87	776.5
88	572.0
89	572.0
90	440.0
91	308.0
92	185.5
93	63.0
94	35.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1430042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.799167702866804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4582466895337	32.769096771973125
2	7.357120674314112	5.709003177058835
3	2.4376322959912855	2.8373431275027046
4	1.2543790320312806	1.9467544970696558
5	0.7801672795112209	1.513492055702261
6	0.5305266336192024	1.2350395097177285
7	0.37934993952369045	1.0302923345156434
8	0.28564720611466593	0.8866299083118627
9	0.22810497909915498	0.7965255004134336
>10	1.7549238670706513	13.867891017870729
>50	0.26379375056050636	7.189353169978643
>100	0.23763668654167167	18.886717519501836
>500	0.023763667919821218	6.368629422747049
>1k	0.008707298169094798	4.963231987636501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2308	0.1613938611593226	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2262	0.15817717241871218	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2191	0.1532122832755961	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2167	0.1515340108891907	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2062	0.14419156919866688	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2034	0.14223358474786055	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2005	0.14020567228095399	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1939	0.13559042321833903	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1936	0.13538063917003837	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1920	0.13426179091243473	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1875	0.13111503018792453	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1807	0.12635992509310914	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1806	0.12628999707700894	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1719	0.12020625967628921	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1676	0.11719935498397949	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1660	0.11608050672637586	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1637	0.11447216235607066	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1593	0.11139532964766069	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1577	0.11027648139005708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	6.992801610022643E-5	0.0	0.0	0.0	0.0
4	6.992801610022643E-5	0.0	0.0	0.0	0.0
5	6.992801610022643E-5	0.0	0.0	0.0	0.0
6	6.992801610022643E-5	0.0	0.0	0.0	0.0
7	6.992801610022643E-5	0.0	0.0	0.0	0.0
8	1.3985603220045286E-4	0.0	0.0	0.0	0.0
9	1.3985603220045286E-4	0.0	0.0	1.3985603220045286E-4	0.0
10	2.097840483006793E-4	0.0	0.0	1.3985603220045286E-4	0.0
11	2.097840483006793E-4	0.0	0.0	2.097840483006793E-4	0.0
12	2.097840483006793E-4	0.0	0.0	2.097840483006793E-4	0.0
13	2.097840483006793E-4	0.0	0.0	2.797120644009057E-4	0.0
14	2.097840483006793E-4	0.0	0.0	4.195680966013586E-4	0.0
15	2.797120644009057E-4	0.0	0.0	4.195680966013586E-4	0.0
16	2.797120644009057E-4	0.0	0.0	8.391361932027172E-4	0.0
17	4.195680966013586E-4	0.0	0.0	0.0013985603220045286	0.0
18	4.195680966013586E-4	0.0	0.0	0.0016083443703052078	0.0
19	4.195680966013586E-4	0.0	0.0	0.002447480563507925	6.992801610022643E-5
20	4.195680966013586E-4	0.0	0.0	0.0033565447728108686	6.992801610022643E-5
21	4.195680966013586E-4	0.0	0.0	0.004964889143116076	6.992801610022643E-5
22	4.195680966013586E-4	0.0	0.0	0.007971793835425813	6.992801610022643E-5
23	4.195680966013586E-4	0.0	0.0	0.009859850270131925	6.992801610022643E-5
24	4.195680966013586E-4	0.0	0.0	0.012237402817539624	6.992801610022643E-5
25	4.195680966013586E-4	0.0	0.0	0.0134961071073437	6.992801610022643E-5
26	4.195680966013586E-4	0.0	0.0	0.015733803622550946	6.992801610022643E-5
27	4.195680966013586E-4	0.0	0.0	0.028250918504491476	1.3985603220045286E-4
28	4.195680966013586E-4	0.0	0.0	0.10587101637574281	1.3985603220045286E-4
29	4.195680966013586E-4	1.3985603220045286E-4	0.0	0.2262171320842325	1.3985603220045286E-4
30	4.195680966013586E-4	1.3985603220045286E-4	0.0	0.3867718570503524	1.3985603220045286E-4
31	4.195680966013586E-4	1.3985603220045286E-4	0.0	0.9329096627931207	1.3985603220045286E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCG	25	0.005496786	29.6	9
GGTATCA	1585	0.0	28.01262	1
TTACGAG	60	1.3377885E-6	24.666668	6
GATCTAA	45	1.3234332E-4	24.666666	1
GTACAAT	55	1.9030804E-5	23.545454	1
GTATAGA	75	3.7420978E-7	22.2	1
CTTATAC	1475	0.0	21.57288	37
TACGAGG	70	5.1033603E-6	21.142859	7
GTAACGT	580	0.0	19.456896	26
TGCGGGT	590	0.0	19.440678	21
TTCTGCG	590	0.0	19.12712	18
GCGGTAA	890	0.0	19.123594	23
GCTTAGG	555	0.0	19.0	1
TAGGACA	605	0.0	18.652893	4
ATAGACT	50	0.0070357006	18.5	4
TCTAGCC	70	1.2194613E-4	18.5	23
GTGCTAT	90	2.152965E-6	18.5	1
ATAAGAG	130	6.9667294E-10	18.5	14
AGTTAGA	80	1.61712E-5	18.5	26
ATTATAC	50	0.0070357006	18.5	3
>>END_MODULE