FastQCFastQC Report
Fri 10 Feb 2017
ERR1631987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887207
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24160.2723152545009226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20140.2270045209291631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18830.21223908287468424No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16840.1898091426239874No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16560.18665317113142704No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16300.18372262617404958No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14790.16670292276774193No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14480.16320881147240723No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14210.1601655532474383No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13750.15498074293823202No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13650.15385361026231759No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12380.1395390252782045No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12010.1353686343773212No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11730.13221266288476083No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11260.12691513930796308No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10600.11947606364692794No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA9940.1120369879858928No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9790.1103462889720212No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9100.10256907350821172No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9040.10189279390266308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.029.6490081
CCGCTTA1050.026.42857425
TATAGAA659.386713E-825.6153852
GGACGTA802.7241185E-823.12527
TATACTG400.001930842123.1255
GCAGTCG3000.022.8166689
TTCTATA502.7014286E-422.22
AGTCGGT3000.022.211
CAGTCGG3100.022.08064510
AGAGTGT855.1773895E-821.7647066
ATTAGAG1059.822543E-1021.1428593
CTCTATG5650.019.9734521
ACGTATA951.6750346E-719.47368429
TCTATGG5850.019.29062
CTTATAC11800.019.28389737
TATACAC3850.019.2207837
TAGAGTG500.007033647518.55
GACGTAT1002.8730938E-718.528
TAGCACT1002.8730938E-718.54
GCTTATT1501.2732926E-1118.527
Produced by FastQC (version 0.11.5)