##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631987.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887207
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60960181783958	34.0	31.0	34.0	31.0	34.0
2	32.77406850937831	34.0	31.0	34.0	31.0	34.0
3	32.861714346257415	34.0	31.0	34.0	31.0	34.0
4	36.29898434074573	37.0	37.0	37.0	35.0	37.0
5	36.21270233440448	37.0	35.0	37.0	35.0	37.0
6	36.26454367470049	37.0	37.0	37.0	35.0	37.0
7	36.25150613103819	37.0	37.0	37.0	35.0	37.0
8	36.23507817228674	37.0	37.0	37.0	35.0	37.0
9	38.06622580750603	39.0	38.0	39.0	35.0	39.0
10	38.00759236570496	39.0	38.0	39.0	35.0	39.0
11	38.06844175034688	39.0	38.0	39.0	37.0	39.0
12	38.01113381657268	39.0	38.0	39.0	35.0	39.0
13	38.06065551782166	39.0	38.0	39.0	35.0	39.0
14	39.441837136091124	40.0	39.0	41.0	37.0	41.0
15	39.44592975483737	40.0	39.0	41.0	37.0	41.0
16	39.39451559782554	40.0	39.0	41.0	37.0	41.0
17	39.36698876361435	40.0	39.0	41.0	37.0	41.0
18	39.40100224637542	40.0	39.0	41.0	37.0	41.0
19	39.431331132418926	41.0	39.0	41.0	37.0	41.0
20	39.428105278700464	41.0	39.0	41.0	37.0	41.0
21	39.389613697817985	40.0	39.0	41.0	37.0	41.0
22	39.36882148134539	40.0	39.0	41.0	36.0	41.0
23	39.303969648571304	40.0	39.0	41.0	36.0	41.0
24	39.3222111637983	40.0	39.0	41.0	36.0	41.0
25	39.262407758279636	40.0	39.0	41.0	36.0	41.0
26	39.20575243432479	40.0	39.0	41.0	36.0	41.0
27	39.09523031265533	40.0	39.0	41.0	35.0	41.0
28	39.09013792722555	40.0	39.0	41.0	35.0	41.0
29	39.06690997704031	40.0	39.0	41.0	35.0	41.0
30	39.00809957540912	40.0	39.0	41.0	35.0	41.0
31	38.91812170102355	40.0	38.0	41.0	35.0	41.0
32	38.87106842033483	40.0	38.0	41.0	35.0	41.0
33	38.81125487062208	40.0	38.0	41.0	35.0	41.0
34	38.78094401870138	40.0	38.0	41.0	35.0	41.0
35	38.706124951674184	40.0	38.0	41.0	35.0	41.0
36	38.65172389307118	40.0	38.0	41.0	35.0	41.0
37	38.60104688082939	40.0	38.0	41.0	35.0	41.0
38	38.571322137900175	40.0	38.0	41.0	35.0	41.0
39	38.5023934662373	40.0	38.0	41.0	35.0	41.0
40	38.182486161628574	40.0	38.0	41.0	34.0	41.0
41	38.24955281011083	40.0	38.0	41.0	34.0	41.0
42	38.22542202665218	40.0	37.0	41.0	34.0	41.0
43	37.767940289019364	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	8.0
19	17.0
20	38.0
21	55.0
22	143.0
23	222.0
24	453.0
25	778.0
26	1214.0
27	1883.0
28	2786.0
29	4190.0
30	6106.0
31	8090.0
32	11174.0
33	15330.0
34	22010.0
35	32718.0
36	50853.0
37	93161.0
38	208168.0
39	427804.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.4419554850221	17.093756023115237	13.303546973817834	27.16074151804483
2	19.598357542264658	20.236089210297035	34.26426978146024	25.901283465978064
3	20.34542107986073	20.78071971929888	30.56006095533511	28.31379824550528
4	14.857862933903812	16.695089195644307	35.035453958320886	33.411593912130996
5	15.912746405292113	33.82446261131844	34.05090356590964	16.211887417479797
6	32.15799694997898	35.09305043806011	16.264749939980184	16.48420267198072
7	29.012845931107396	29.404411822720068	20.70351113099874	20.8792311151738
8	26.63493412473076	31.929639869838716	20.657185978018656	20.77824002741187
9	27.953792068818213	13.608323649385094	18.61730126114875	39.820583020647945
10	18.70871172116541	24.535311376037384	30.471693753543423	26.284283149253785
11	37.3729017016322	20.687280420465573	20.046280067673045	21.89353781022918
12	21.977509194584805	25.075207927800392	27.627036306070625	25.320246571544182
13	33.8618834161588	18.61493428252933	22.231903039538686	25.29127926177318
14	23.27359905861879	21.254904436056073	24.670905436949887	30.800591068375248
15	28.461452626050065	25.430931000318978	21.358375215705017	24.74924115792594
16	24.21836166757025	25.403992529364622	23.661783552203712	26.71586225086141
17	25.455389779386323	24.928117113593558	23.239785078341356	26.376708028678763
18	25.456516912062238	22.529578779247682	24.854064496785984	27.159839811904103
19	26.985246960404957	23.411221958347937	25.054806826366338	24.548724254880767
20	28.63164966011314	22.030822570155557	24.30357289786938	25.033954871861923
21	26.13865760752564	23.685566051665507	23.43094678017644	26.74482956063241
22	26.852245304647056	24.044670522211838	23.150967023479303	25.952117149661802
23	26.314490304968285	23.354639898017034	24.23154911987845	26.099320677136227
24	26.431486676728206	22.941545772294404	24.608913139774597	26.018054411202797
25	26.227024809317328	23.334238796582984	24.879988548332012	25.55874784576767
26	26.268165151988203	23.891718618090255	24.1360809822285	25.704035247693042
27	25.679576468625697	23.200786287754717	25.376490492072314	25.74314675154727
28	25.10834562847227	24.296697388546303	23.908963748031745	26.685993234949677
29	25.786654072837567	24.723204393112315	23.95945929191271	25.530682242137402
30	25.535528912643834	23.750150753995403	25.083435996334565	25.630884337026195
31	26.332524427782918	23.620530496265246	24.463625737849227	25.583319338102605
32	24.090995675191923	23.75691355005089	24.738195257701978	27.41389551705521
33	24.40839623672942	23.100358766330746	26.35709592011785	26.134149076821984
34	24.915155087820544	23.049525082647005	25.713390448903127	26.32192938062932
35	24.47557334421392	24.33344191378111	26.092107028010375	25.098877713994593
36	24.613421670478253	23.325898014781217	26.57350539389342	25.487174920847107
37	25.286996157604708	22.826465526083542	25.917739603046414	25.96879871326534
38	24.179701016786385	22.29784030107968	27.112612952783284	26.409845729350646
39	23.927786863719515	21.281504767207654	28.12376367634611	26.666944692726723
40	23.581644418946198	22.14601552963401	28.812667167864998	25.459672883554795
41	22.04694056742113	21.65999591977971	29.794625155121636	26.49843835767752
42	20.90887470455035	22.01166131466501	30.31288075950708	26.766583221277564
43	20.057776820967373	22.696168988747832	30.151362647048547	27.094691543236245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	21.5
2	25.0
3	64.5
4	104.0
5	104.0
6	139.0
7	174.0
8	194.5
9	215.0
10	356.5
11	498.0
12	498.0
13	979.5
14	1461.0
15	2967.0
16	4473.0
17	4462.5
18	4452.0
19	4452.0
20	4917.0
21	5382.0
22	4901.5
23	4421.0
24	4945.5
25	5470.0
26	5470.0
27	6007.5
28	6545.0
29	8648.5
30	10752.0
31	12058.0
32	13364.0
33	13364.0
34	15423.0
35	17482.0
36	18980.0
37	20478.0
38	24244.0
39	28010.0
40	28010.0
41	32991.0
42	37972.0
43	41637.5
44	45303.0
45	58209.0
46	71115.0
47	71115.0
48	77531.0
49	83947.0
50	91672.0
51	99397.0
52	99441.5
53	99486.0
54	99486.0
55	88063.0
56	76640.0
57	70758.0
58	64876.0
59	59299.5
60	53723.0
61	53723.0
62	48293.5
63	42864.0
64	35175.5
65	27487.0
66	23711.0
67	19935.0
68	19935.0
69	17181.5
70	14428.0
71	12394.5
72	10361.0
73	8265.5
74	6170.0
75	6170.0
76	5040.5
77	3911.0
78	3219.5
79	2528.0
80	2013.5
81	1499.0
82	1499.0
83	1237.5
84	976.0
85	792.5
86	609.0
87	484.0
88	359.0
89	359.0
90	286.5
91	214.0
92	139.0
93	64.0
94	39.5
95	15.0
96	15.0
97	9.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	887207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.27510566385324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.77426988719719	41.022627778209646
2	6.219956362662985	5.8809818853889775
3	1.9451314266741349	2.7586888117830397
4	1.0956602818212777	2.071898223791525
5	0.6695070701168265	1.5825508741234888
6	0.4650126296641555	1.3190112721439524
7	0.3449801337691469	1.141628059310665
8	0.2523587180470926	0.9544228048696679
9	0.21783167548494087	0.9268213927936291
>10	1.625838062187146	15.648845232932901
>50	0.2300341271261479	7.5386347083373
>100	0.1448181280418483	12.965180198108877
>500	0.010771596300383634	3.453699853726687
>1k	0.0038299009068030695	2.7350089044796952
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2416	0.2723152545009226	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.2270045209291631	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1883	0.21223908287468424	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1684	0.1898091426239874	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1656	0.18665317113142704	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1630	0.18372262617404958	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1479	0.16670292276774193	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1448	0.16320881147240723	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1421	0.1601655532474383	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1375	0.15498074293823202	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1365	0.15385361026231759	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1238	0.1395390252782045	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1201	0.1353686343773212	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1173	0.13221266288476083	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1126	0.12691513930796308	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1060	0.11947606364692794	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	994	0.1120369879858928	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	979	0.1103462889720212	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	910	0.10256907350821172	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	904	0.10189279390266308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1271326759144145E-4
2	0.0	0.0	0.0	0.0	1.1271326759144145E-4
3	0.0	0.0	0.0	0.0	1.1271326759144145E-4
4	0.0	0.0	1.1271326759144145E-4	0.0	1.1271326759144145E-4
5	1.1271326759144145E-4	0.0	1.1271326759144145E-4	0.0	1.1271326759144145E-4
6	1.1271326759144145E-4	0.0	1.1271326759144145E-4	0.0	2.254265351828829E-4
7	1.1271326759144145E-4	0.0	1.1271326759144145E-4	0.0	2.254265351828829E-4
8	2.254265351828829E-4	0.0	1.1271326759144145E-4	0.0	2.254265351828829E-4
9	2.254265351828829E-4	0.0	1.1271326759144145E-4	0.0	2.254265351828829E-4
10	2.254265351828829E-4	0.0	1.1271326759144145E-4	0.0	2.254265351828829E-4
11	2.254265351828829E-4	0.0	1.1271326759144145E-4	1.1271326759144145E-4	2.254265351828829E-4
12	2.254265351828829E-4	0.0	1.1271326759144145E-4	2.254265351828829E-4	2.254265351828829E-4
13	2.254265351828829E-4	0.0	1.1271326759144145E-4	2.254265351828829E-4	2.254265351828829E-4
14	2.254265351828829E-4	0.0	1.1271326759144145E-4	2.254265351828829E-4	2.254265351828829E-4
15	2.254265351828829E-4	0.0	1.1271326759144145E-4	5.635663379572073E-4	2.254265351828829E-4
16	2.254265351828829E-4	0.0	1.1271326759144145E-4	0.001465272478688739	2.254265351828829E-4
17	2.254265351828829E-4	0.0	1.1271326759144145E-4	0.002930544957377478	2.254265351828829E-4
18	2.254265351828829E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.003494111295334685	2.254265351828829E-4
19	2.254265351828829E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.004057677633291892	2.254265351828829E-4
20	2.254265351828829E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.005297523576797749	2.254265351828829E-4
21	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.008678921604540993	3.381398027743244E-4
22	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.013300165575790092	3.381398027743244E-4
23	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.01634342380075901	3.381398027743244E-4
24	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.01949939529331937	3.381398027743244E-4
25	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.021415520842373876	3.381398027743244E-4
26	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.02468420560252568	3.381398027743244E-4
27	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.04497259376898514	3.381398027743244E-4
28	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.15329004392436038	3.381398027743244E-4
29	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.33949236198542165	3.381398027743244E-4
30	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	0.567060449252542	3.381398027743244E-4
31	3.381398027743244E-4	1.1271326759144145E-4	1.1271326759144145E-4	1.2494265712511285	3.381398027743244E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1510	0.0	29.649008	1
CCGCTTA	105	0.0	26.428574	25
TATAGAA	65	9.386713E-8	25.615385	2
GGACGTA	80	2.7241185E-8	23.125	27
TATACTG	40	0.0019308421	23.125	5
GCAGTCG	300	0.0	22.816668	9
TTCTATA	50	2.7014286E-4	22.2	2
AGTCGGT	300	0.0	22.2	11
CAGTCGG	310	0.0	22.080645	10
AGAGTGT	85	5.1773895E-8	21.764706	6
ATTAGAG	105	9.822543E-10	21.142859	3
CTCTATG	565	0.0	19.973452	1
ACGTATA	95	1.6750346E-7	19.473684	29
TCTATGG	585	0.0	19.2906	2
CTTATAC	1180	0.0	19.283897	37
TATACAC	385	0.0	19.22078	37
TAGAGTG	50	0.0070336475	18.5	5
GACGTAT	100	2.8730938E-7	18.5	28
TAGCACT	100	2.8730938E-7	18.5	4
GCTTATT	150	1.2732926E-11	18.5	27
>>END_MODULE