FastQCFastQC Report
Fri 10 Feb 2017
ERR1631986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466777
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA34270.2336415146951445No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA33530.2285964396769243No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA27530.18769042601567928No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA26300.17930469321512404No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25140.17139619724061667No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA23780.16212416747740113No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC23490.1601470434837743No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA23020.1569427390803101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.1445345816030658No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20600.14044398023694127No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC19810.135058021771544No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC19380.13212642412582146No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19150.13055836026880707No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT18790.12810399944913237No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA18450.12578599200832846No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA18040.12299074774147671No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17890.12196809739994559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17720.12080909367954365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17620.12012732678518957No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT17500.11930920651196467No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16510.11255971425785925No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA16490.11242336087898842No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16410.11187794736350516No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA16400.11180977067406975No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC15940.10867364296004095No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG15240.10390127469956237No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC15120.10308315442633746No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14750.10056061691722737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.030.0748671
ATACGGC2400.023.12499829
GCGGTAA13600.021.22058923
AAGTTAG450.003826304820.55555525
TACTACC450.003826304820.5555557
GTCTTAC450.003826304820.5555551
GGTCTAT555.144025E-420.1818181
CGCGGTA14700.019.88435422
ATTACCT2000.019.42533
AAACACG1052.26064E-819.3809515
CAGTCGG3750.019.24000210
AACTCCG15100.019.23515
AGCCGCG15200.019.23026319
GGTAATA15050.019.17607925
GCAGTCG3800.018.9868439
TATACAC3950.018.73417737
CGGTAAT15450.018.67961124
CCGCGGT15750.018.6761921
CTTATAC13600.018.6360337
CGTGCCA15650.018.55910510
Produced by FastQC (version 0.11.5)