##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631986.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1466777
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.545444876760406	34.0	31.0	34.0	31.0	34.0
2	32.712632526962174	34.0	31.0	34.0	31.0	34.0
3	32.80674976496086	34.0	31.0	34.0	31.0	34.0
4	36.261857119384885	37.0	37.0	37.0	35.0	37.0
5	36.16915318415819	37.0	35.0	37.0	35.0	37.0
6	36.21705344438862	37.0	36.0	37.0	35.0	37.0
7	36.209083589393614	37.0	36.0	37.0	35.0	37.0
8	36.19129083698476	37.0	36.0	37.0	35.0	37.0
9	38.00981948857938	39.0	38.0	39.0	35.0	39.0
10	37.93600663222835	39.0	38.0	39.0	35.0	39.0
11	38.007901678305565	39.0	38.0	39.0	35.0	39.0
12	37.94122419427084	39.0	38.0	39.0	35.0	39.0
13	37.99264032637545	39.0	38.0	39.0	35.0	39.0
14	39.37199383410021	40.0	39.0	41.0	37.0	41.0
15	39.3750979187702	40.0	39.0	41.0	37.0	41.0
16	39.32265572748959	40.0	39.0	41.0	36.0	41.0
17	39.294142872433916	40.0	39.0	41.0	36.0	41.0
18	39.31806811805748	40.0	39.0	41.0	36.0	41.0
19	39.341854965001495	40.0	39.0	41.0	36.0	41.0
20	39.33267838260349	40.0	39.0	41.0	36.0	41.0
21	39.29797031177882	40.0	39.0	41.0	36.0	41.0
22	39.272558132558665	40.0	39.0	41.0	36.0	41.0
23	39.20449325289393	40.0	39.0	41.0	36.0	41.0
24	39.226205483178425	40.0	39.0	41.0	36.0	41.0
25	39.168332336817386	40.0	39.0	41.0	36.0	41.0
26	39.11040873970617	40.0	39.0	41.0	36.0	41.0
27	38.98641034049484	40.0	39.0	41.0	35.0	41.0
28	38.9831071798917	40.0	39.0	41.0	35.0	41.0
29	38.96151494058061	40.0	38.0	41.0	35.0	41.0
30	38.923123964992634	40.0	38.0	41.0	35.0	41.0
31	38.84045086608258	40.0	38.0	41.0	35.0	41.0
32	38.79072347057528	40.0	38.0	41.0	35.0	41.0
33	38.73462019107199	40.0	38.0	41.0	35.0	41.0
34	38.70650889671709	40.0	38.0	41.0	35.0	41.0
35	38.63474952225185	40.0	38.0	41.0	35.0	41.0
36	38.58367018299305	40.0	38.0	41.0	35.0	41.0
37	38.53901308787907	40.0	38.0	41.0	35.0	41.0
38	38.50191746939037	40.0	38.0	41.0	35.0	41.0
39	38.42441898120846	40.0	38.0	41.0	34.0	41.0
40	38.114234133750394	40.0	37.0	41.0	34.0	41.0
41	38.18985980827351	40.0	37.0	41.0	34.0	41.0
42	38.17411781068288	40.0	37.0	41.0	34.0	41.0
43	37.687362837022945	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	4.0
17	3.0
18	7.0
19	24.0
20	50.0
21	94.0
22	249.0
23	394.0
24	741.0
25	1212.0
26	2094.0
27	3198.0
28	4919.0
29	7257.0
30	10313.0
31	14304.0
32	19250.0
33	26603.0
34	37944.0
35	57381.0
36	88522.0
37	160502.0
38	370520.0
39	661190.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.90897662016789	16.869299150450274	12.77038022821465	27.451344001167183
2	19.99697295498907	19.999768199255918	33.87345179260378	26.12980705315123
3	20.554453744502403	20.464255984379356	30.072737709958634	28.908552561159606
4	14.825702884623906	16.174237801656286	35.28784539163077	33.71221392208904
5	15.98886538308141	33.94776438408838	34.093457969411844	15.969912263418365
6	32.77635250620919	35.28954980886665	16.102652277749105	15.831445407175051
7	29.50359870655185	28.96827534110502	20.330084259570473	21.198041692772655
8	26.677470399385868	32.00595591558908	20.51634297510801	20.80023070991705
9	27.96560076957847	13.351791035719812	18.006486330232885	40.67612186446883
10	18.633098282833725	24.138297778053513	30.271131876215673	26.957472062897086
11	38.48574118628803	20.153779340690505	19.799874145831303	21.56060532719016
12	22.226691582974098	24.267492604533615	27.66016920090784	25.845646611584446
13	34.19585935694383	18.166497020337786	21.864741538761514	25.77290208395687
14	23.338721564355044	20.259725916073133	24.241789992616464	32.15976252695536
15	28.60462087965655	24.929079198814815	21.00960132317319	25.456698598355445
16	24.65998580561326	24.863015986751904	23.094171779350237	27.382826428284602
17	26.084878614813295	24.40916376518039	22.494489619076383	27.011468000929927
18	25.94313927747708	22.147197563092412	24.267288074465306	27.6423750849652
19	27.546177776171838	22.98508907625358	24.17906743833589	25.28966570923869
20	29.065018063413866	21.514790591889565	23.62819978769779	25.791991556998777
21	26.70235489102979	23.102352982082483	22.685861586321572	27.509430540566154
22	27.291265134372843	23.574135672975512	22.216056019422176	26.91854317322947
23	27.085030648830738	22.913503552346405	23.382695529040884	26.618770269781976
24	27.15443451867598	22.26971107400784	23.542774395835224	27.033080011480955
25	27.277834326554068	22.914935262824546	23.939903611796474	25.86732679882491
26	27.239450850401937	23.274226416149148	23.227116323749282	26.25920640969963
27	26.429920840045895	22.587141740019103	24.66162204615971	26.32131537377529
28	25.840192476429614	23.738850554651457	23.02429067267894	27.396666296239985
29	26.463327417869248	24.234631440225744	23.028994864249984	26.27304627765502
30	26.109285869631172	23.28724816383131	24.342077902775948	26.261388063761565
31	27.198203953293515	23.07835478740122	23.492255468963585	26.231185790341684
32	24.623579453454752	23.227184500438717	23.913519232984974	28.23571681312156
33	25.138108928623776	22.777900117059378	25.41340640056396	26.670584553752892
34	25.47272012037276	22.743470888894496	24.750456272494045	27.033352718238696
35	25.133881973878786	24.21649644083593	24.950077619160922	25.699543966124367
36	25.525693408064075	23.142849935607117	25.465357037913737	25.866099618415067
37	26.34804063603397	22.647478110169438	24.6614175160914	26.343063737705187
38	25.235260711069234	22.254644025642616	25.796559395190954	26.713535868097193
39	24.736207344402047	21.235334341893825	26.76732727606173	27.261131037642393
40	24.632169716323613	21.970483584075833	27.58026612088954	25.81708057871101
41	23.11401119597594	21.40011740025921	28.478971241027097	27.006900162737757
42	22.0535909684976	21.622577937886945	29.27520679694323	27.04862429667223
43	21.068437806155945	22.358272593584438	29.05779133433371	27.515498265925903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	11.5
2	12.0
3	46.0
4	80.0
5	80.0
6	107.5
7	135.0
8	168.0
9	201.0
10	297.0
11	393.0
12	393.0
13	781.5
14	1170.0
15	2495.0
16	3820.0
17	3861.0
18	3902.0
19	3902.0
20	4267.5
21	4633.0
22	4499.5
23	4366.0
24	5008.0
25	5650.0
26	5650.0
27	6496.5
28	7343.0
29	10171.5
30	13000.0
31	14986.5
32	16973.0
33	16973.0
34	20608.0
35	24243.0
36	26760.0
37	29277.0
38	36480.0
39	43683.0
40	43683.0
41	52259.0
42	60835.0
43	67244.5
44	73654.0
45	95306.0
46	116958.0
47	116958.0
48	128257.0
49	139556.0
50	151946.0
51	164336.0
52	166689.0
53	169042.0
54	169042.0
55	151223.0
56	133404.0
57	123758.0
58	114112.0
59	105210.0
60	96308.0
61	96308.0
62	86562.0
63	76816.0
64	63900.5
65	50985.0
66	44013.5
67	37042.0
68	37042.0
69	31778.5
70	26515.0
71	22705.5
72	18896.0
73	15273.0
74	11650.0
75	11650.0
76	9457.0
77	7264.0
78	5867.0
79	4470.0
80	3568.5
81	2667.0
82	2667.0
83	2126.5
84	1586.0
85	1247.0
86	908.0
87	713.5
88	519.0
89	519.0
90	389.0
91	259.0
92	171.5
93	84.0
94	49.5
95	15.0
96	15.0
97	9.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1466777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.42008438421024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.47116487718348	37.54585275768264
2	6.601720670776749	5.73294537212223
3	2.0557459311633695	2.6778198541083107
4	1.0706469909200944	1.859503307658051
5	0.6443152884924079	1.3988112098188556
6	0.46136537621707446	1.2019514140378966
7	0.31870951140587134	0.9686875715505345
8	0.2631593605941745	0.9141121314795093
9	0.19291447829801853	0.7538726633972274
>10	1.4854624339731803	13.155272109221094
>50	0.225896925874044	6.909905128413879
>100	0.18233124871429215	15.313278414523513
>500	0.01739499822972321	5.118753181962974
>1k	0.00917190815749042	6.449234884023318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3427	0.2336415146951445	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3353	0.2285964396769243	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2753	0.18769042601567928	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2630	0.17930469321512404	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2514	0.17139619724061667	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2378	0.16212416747740113	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2349	0.1601470434837743	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2302	0.1569427390803101	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2120	0.1445345816030658	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2060	0.14044398023694127	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1981	0.135058021771544	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1938	0.13212642412582146	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1915	0.13055836026880707	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1879	0.12810399944913237	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1845	0.12578599200832846	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1804	0.12299074774147671	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1789	0.12196809739994559	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1772	0.12080909367954365	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1762	0.12012732678518957	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1750	0.11930920651196467	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1651	0.11255971425785925	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1649	0.11242336087898842	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1641	0.11187794736350516	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1640	0.11180977067406975	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1594	0.10867364296004095	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1524	0.10390127469956237	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1512	0.10308315442633746	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1475	0.10056061691722737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.817668943540839E-5	0.0	0.0	0.0	0.0
6	1.3635337887081677E-4	0.0	0.0	0.0	0.0
7	1.3635337887081677E-4	0.0	6.817668943540839E-5	0.0	0.0
8	3.408834471770419E-4	0.0	6.817668943540839E-5	0.0	0.0
9	3.408834471770419E-4	0.0	6.817668943540839E-5	0.0	0.0
10	4.090601366124503E-4	0.0	6.817668943540839E-5	6.817668943540839E-5	0.0
11	4.090601366124503E-4	0.0	6.817668943540839E-5	1.3635337887081677E-4	0.0
12	4.7723682604785866E-4	0.0	6.817668943540839E-5	1.3635337887081677E-4	0.0
13	4.7723682604785866E-4	0.0	6.817668943540839E-5	2.0453006830622515E-4	0.0
14	5.454135154832671E-4	0.0	6.817668943540839E-5	2.7270675774163355E-4	0.0
15	6.135902049186755E-4	0.0	6.817668943540839E-5	5.454135154832671E-4	0.0
16	6.135902049186755E-4	0.0	6.817668943540839E-5	8.181202732249006E-4	0.0
17	6.135902049186755E-4	0.0	6.817668943540839E-5	0.0013635337887081677	0.0
18	6.817668943540838E-4	0.0	6.817668943540839E-5	0.001772593925320618	0.0
19	6.817668943540838E-4	0.0	6.817668943540839E-5	0.002318007440803885	0.0
20	7.499435837894922E-4	0.0	6.817668943540839E-5	0.0033406577823350105	0.0
21	7.499435837894922E-4	0.0	6.817668943540839E-5	0.006613138875234613	0.0
22	8.181202732249006E-4	0.0	6.817668943540839E-5	0.009612913210392583	0.0
23	8.181202732249006E-4	0.0	6.817668943540839E-5	0.011453683825148608	0.0
24	8.181202732249006E-4	0.0	6.817668943540839E-5	0.013839867955387901	0.0
25	8.181202732249006E-4	0.0	6.817668943540839E-5	0.015067048365225252	0.0
26	8.181202732249006E-4	0.0	6.817668943540839E-5	0.01683964229054587	0.0
27	8.86296962660309E-4	0.0	6.817668943540839E-5	0.02870238625230693	0.0
28	8.86296962660309E-4	1.3635337887081677E-4	6.817668943540839E-5	0.10103785374327522	0.0
29	8.86296962660309E-4	1.3635337887081677E-4	6.817668943540839E-5	0.22668749237273286	0.0
30	9.544736520957173E-4	1.3635337887081677E-4	6.817668943540839E-5	0.3901751936388422	0.0
31	9.544736520957173E-4	1.3635337887081677E-4	6.817668943540839E-5	0.8929101015355436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1870	0.0	30.074867	1
ATACGGC	240	0.0	23.124998	29
GCGGTAA	1360	0.0	21.220589	23
AAGTTAG	45	0.0038263048	20.555555	25
TACTACC	45	0.0038263048	20.555555	7
GTCTTAC	45	0.0038263048	20.555555	1
GGTCTAT	55	5.144025E-4	20.181818	1
CGCGGTA	1470	0.0	19.884354	22
ATTACCT	200	0.0	19.425	33
AAACACG	105	2.26064E-8	19.38095	15
CAGTCGG	375	0.0	19.240002	10
AACTCCG	1510	0.0	19.2351	5
AGCCGCG	1520	0.0	19.230263	19
GGTAATA	1505	0.0	19.176079	25
GCAGTCG	380	0.0	18.986843	9
TATACAC	395	0.0	18.734177	37
CGGTAAT	1545	0.0	18.679611	24
CCGCGGT	1575	0.0	18.67619	21
CTTATAC	1360	0.0	18.63603	37
CGTGCCA	1565	0.0	18.559105	10
>>END_MODULE