FastQCFastQC Report
Fri 10 Feb 2017
ERR1631985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19548
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1130.5780642520974013No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA920.4706363822385922No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT830.42459586658481685No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA770.39390218948229994No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC750.38367096378146104No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT750.38367096378146104No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG710.36320851237978313No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA650.3325148352772662No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC640.3273992224268467No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA630.32228360957642727No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA570.29158993247391035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA540.2762430939226519No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA540.2762430939226519No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA520.26601186822181294No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA480.24554941682013504No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA470.24043380396971556No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT450.23020257826887663No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA430.21997135256803768No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT420.21485573971761818No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC420.21485573971761818No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC420.21485573971761818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT410.2097401268671987No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC410.2097401268671987No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT400.20462451401677922No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT390.19950890116635975No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC380.19439328831594027No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC380.19439328831594027No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA380.19439328831594027No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA370.18927767546552077No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG370.18927767546552077No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG360.1841620626151013No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG360.1841620626151013No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC340.17393083691426234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT330.16881522406384286No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA320.16369961121342336No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA320.16369961121342336No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA320.16369961121342336No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC320.16369961121342336No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG310.15858399836300388No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC300.1534683855125844No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC300.1534683855125844No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT300.1534683855125844No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA290.14835277266216493No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC290.14835277266216493No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT280.14323715981174545No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG270.13812154696132595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270.13812154696132595No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT260.13300593411090647No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC260.13300593411090647No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT250.127890321260487No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC250.127890321260487No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT250.127890321260487No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG250.127890321260487No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA250.127890321260487No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT250.127890321260487No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA240.12277470841006752No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC240.12277470841006752No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG240.12277470841006752No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC240.12277470841006752No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC240.12277470841006752No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC240.12277470841006752No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG230.11765909555964806No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC230.11765909555964806No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT230.11765909555964806No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA220.11254348270922858No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA220.11254348270922858No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA220.11254348270922858No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC220.11254348270922858No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC210.10742786985880909No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA210.10742786985880909No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC210.10742786985880909No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG210.10742786985880909No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT210.10742786985880909No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG210.10742786985880909No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT210.10742786985880909No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC200.10231225700838961No Hit
GTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGAC200.10231225700838961No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA200.10231225700838961No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT200.10231225700838961No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG200.10231225700838961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA602.910383E-1133.9166641
ATAGGTT250.005366502329.617
CATAGGT250.005366502329.616
TAATACG250.005366502329.627
GTAATAC250.005366502329.626
GGTAATA250.005366502329.625
TAGGTTA250.005366502329.618
ACGGGCC358.5593713E-426.4285715
GTATCAA808.367351E-1025.43752
GGCCACT400.001864312923.12518
GGGCCAC400.001864312923.12517
ATTAACT450.003694566720.5555554
TACGGGC554.8983906E-420.18181814
CTCTTAT758.524885E-619.73333437
AACGCAG1208.9574314E-816.9583327
TCAACGC1208.9574314E-816.9583325
ATCAACG1208.9574314E-816.9583324
ACGCAGA1302.2303539E-715.6538468
CAGAGTA1302.2303539E-715.65384611
AGAGTAC1302.2303539E-715.65384612
Produced by FastQC (version 0.11.5)