FastQCFastQC Report
Fri 10 Feb 2017
ERR1631984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89892
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2610.29034841810172207No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2220.24696302229341877No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2110.2347261157833845No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2000.22248920927335022No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1890.210252302763316No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1770.19690295020691495No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1740.19356561206781472No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1670.18577848974324745No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1520.16909179904774618No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1470.16352956881591243No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1430.15907978463044542No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1330.1479553241667779No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1310.1457304320740444No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1280.14239309393494415No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1180.13126863347127662No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1100.12236906510034262No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1090.12125661905397589No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1080.12014417300760913No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1060.11791928091487563No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1040.11569438882214213No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1030.11458194277577537No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1020.11346949672940863No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC950.10568237440484138No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT940.10456992835847462No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA940.10456992835847462No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA930.10345748231210786No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC910.10123259021937436No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC910.10123259021937436No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG910.10123259021937436No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC900.1001201441730076No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA900.1001201441730076No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG900.1001201441730076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1350.032.888891
ATCTAAG405.8803278E-527.7532
ATACGGA659.191899E-825.61538529
TAATACG751.3382305E-824.66666627
CATCTAA451.309998E-424.66666631
TCTAAGT451.309998E-424.66666633
AATACGG751.3382305E-824.66666628
TACGGAG701.8822175E-723.78571330
AAGTACC400.001917440223.12536
GCGGTAA802.6557245E-823.12523
CTAAGTA400.001917440223.12534
AGGGTGC400.001917440223.12535
TAAGTAC400.001917440223.12535
CTTATAC753.6620077E-722.237
ACATCTA502.675337E-422.19999930
GAGGGTG502.675337E-422.19999934
CGCGGTA855.0480594E-821.76470622
GTAATAC855.0480594E-821.76470626
CGGTAAT855.0480594E-821.76470624
GTATCAA2050.021.6585372
Produced by FastQC (version 0.11.5)