##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631984.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89892
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63040092555511	34.0	31.0	34.0	31.0	34.0
2	32.77735504828016	34.0	31.0	34.0	31.0	34.0
3	32.870678147109864	34.0	31.0	34.0	31.0	34.0
4	36.30633426778801	37.0	37.0	37.0	35.0	37.0
5	36.20644773728474	37.0	35.0	37.0	35.0	37.0
6	36.264573043207406	37.0	37.0	37.0	35.0	37.0
7	36.2600120144173	37.0	37.0	37.0	35.0	37.0
8	36.23865305032706	37.0	37.0	37.0	35.0	37.0
9	38.07249810884172	39.0	38.0	39.0	37.0	39.0
10	38.00305922662751	39.0	38.0	39.0	35.0	39.0
11	38.07922840742224	39.0	38.0	39.0	37.0	39.0
12	38.010000889956835	39.0	38.0	39.0	35.0	39.0
13	38.06546744982868	39.0	38.0	39.0	37.0	39.0
14	39.47047568192943	40.0	39.0	41.0	37.0	41.0
15	39.470130823655055	41.0	39.0	41.0	37.0	41.0
16	39.411271303341785	40.0	39.0	41.0	37.0	41.0
17	39.3814132514573	40.0	39.0	41.0	36.0	41.0
18	39.39811106661327	41.0	39.0	41.0	37.0	41.0
19	39.4270346638188	41.0	39.0	41.0	37.0	41.0
20	39.415787834290036	41.0	39.0	41.0	37.0	41.0
21	39.367552173719574	40.0	39.0	41.0	36.0	41.0
22	39.3716459751702	40.0	39.0	41.0	36.0	41.0
23	39.29704534330085	40.0	39.0	41.0	36.0	41.0
24	39.30218484403506	40.0	39.0	41.0	36.0	41.0
25	39.21446847327905	40.0	39.0	41.0	36.0	41.0
26	39.16558759400169	40.0	39.0	41.0	36.0	41.0
27	39.058503537578424	40.0	39.0	41.0	35.0	41.0
28	39.07208650380456	40.0	39.0	41.0	35.0	41.0
29	39.05231833756063	40.0	39.0	41.0	35.0	41.0
30	39.01931206336493	40.0	39.0	41.0	35.0	41.0
31	38.94109598184488	40.0	38.0	41.0	35.0	41.0
32	38.898188937836515	40.0	38.0	41.0	35.0	41.0
33	38.837315890179326	40.0	38.0	41.0	35.0	41.0
34	38.82432252035776	40.0	38.0	41.0	35.0	41.0
35	38.747107640279445	40.0	38.0	41.0	35.0	41.0
36	38.709807324344766	40.0	38.0	41.0	35.0	41.0
37	38.66173853068126	40.0	38.0	41.0	35.0	41.0
38	38.60577137008855	40.0	38.0	41.0	35.0	41.0
39	38.54720108574734	40.0	38.0	41.0	35.0	41.0
40	38.24718551150269	40.0	38.0	41.0	34.0	41.0
41	38.32016197214435	40.0	38.0	41.0	34.0	41.0
42	38.32196413473947	40.0	37.0	41.0	34.0	41.0
43	37.83166466426378	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	5.0
21	9.0
22	13.0
23	36.0
24	48.0
25	74.0
26	123.0
27	192.0
28	296.0
29	415.0
30	624.0
31	767.0
32	1092.0
33	1525.0
34	2277.0
35	3345.0
36	5155.0
37	8995.0
38	21048.0
39	43849.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.42312997819606	15.853468606772573	12.81649090019134	28.90691051484003
2	20.73043207404441	19.480042717928182	32.08516886930984	27.704356338717574
3	21.205446535843013	19.445556890490813	29.111600587371512	30.23739598629466
4	15.814532995149735	16.02144795977395	33.60143283050772	34.56258621456859
5	16.80127263827704	32.95398923152227	33.637031103991454	16.60770702620923
6	33.955190673252346	33.900680816980376	15.669915009122057	16.47421350064522
7	30.308592533262136	27.672095403372936	20.05851466203889	21.960797401326033
8	27.421795042940417	30.338628576514036	20.852801139144752	21.386775241400795
9	28.420771592577758	12.756418813687537	17.406443287500554	41.416366306234146
10	19.176344947270056	23.53713344902772	29.653361811952124	27.6331597917501
11	39.31940550883282	18.893783651492903	18.841498687313667	22.94531215236061
12	22.673875317047123	23.442575535086547	26.586348062118986	27.297201085747343
13	35.34908556934989	17.15280558892894	20.957371067503225	26.54073777421795
14	24.227962443821475	19.876073510434743	23.013171361188984	32.8827926845548
15	29.58772749521648	23.97654963734259	19.999555021581454	26.436167845859476
16	25.620744893872647	23.959862946647085	22.206647977573088	28.21274418190718
17	27.359498064343878	23.556045031815955	21.259956392114983	27.82450051172518
18	26.572998709562583	21.395674809771727	23.19561251279313	28.835713967872557
19	27.946869576825524	22.30899301383883	23.06879366350732	26.67534374582833
20	30.09834023049882	20.289903439683172	22.809593734703867	26.802162595114137
21	27.12922173274596	21.793930494371022	21.829528767854757	29.247319005028256
22	28.70555778044765	22.680549993325325	20.927335024251324	27.686557201975702
23	28.199394829350776	22.099853157121878	21.977484092021538	27.723267921505805
24	28.104836915409603	21.238819917234014	22.690562007742624	27.965781159613755
25	28.20718195167534	21.85066524273573	22.75953366261736	27.182619142971564
26	28.118186267966006	22.59266675566235	22.10652783340008	27.182619142971564
27	27.05802518577849	21.34338984559249	24.097806256396563	27.500778712232453
28	26.42726827748854	22.82961776353847	21.855115026921194	28.887998932051794
29	27.338361589462913	23.42143906020558	22.18328661059939	27.056912739732127
30	26.90673252347261	22.105415387353712	23.269033951853334	27.71881813732034
31	28.003604325190228	22.3534908556935	22.54483157566858	27.09807324344769
32	25.59182129666711	22.232234236639524	22.727272727272727	29.44867173942064
33	26.049036621723847	21.667111645085214	24.47603791216126	27.807813821029683
34	26.61972144350999	21.9318738041205	23.430338628576514	28.018066123792995
35	25.92889244871624	23.382503448582746	24.44933920704846	26.23926489565256
36	26.115783384505853	22.326792150580697	24.67071597027544	26.88670849463801
37	26.987941084857386	22.028656610154407	24.270235393583413	26.713166911404794
38	26.074622880790283	21.374538334890758	25.080096115338407	27.470742668980552
39	25.34930805855916	20.386686245717083	26.331597917501	27.932407778222757
40	25.096782806033907	21.649312508343346	26.79437547278957	26.45952921283318
41	23.252347261157833	20.489031281982825	28.347350153517553	27.91127130334179
42	22.24669603524229	20.885062074489387	28.90691051484003	27.961331375428294
43	21.187647399101145	21.689360566012546	28.956970586926534	28.16602144795977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	2.0
4	4.0
5	4.0
6	4.0
7	4.0
8	4.0
9	4.0
10	9.5
11	15.0
12	15.0
13	26.0
14	37.0
15	73.0
16	109.0
17	113.0
18	117.0
19	117.0
20	144.5
21	172.0
22	142.5
23	113.0
24	147.5
25	182.0
26	182.0
27	190.0
28	198.0
29	284.0
30	370.0
31	453.0
32	536.0
33	536.0
34	720.0
35	904.0
36	1015.0
37	1126.0
38	1483.5
39	1841.0
40	1841.0
41	2257.5
42	2674.0
43	3108.0
44	3542.0
45	5060.5
46	6579.0
47	6579.0
48	7335.0
49	8091.0
50	9312.5
51	10534.0
52	11063.0
53	11592.0
54	11592.0
55	10433.5
56	9275.0
57	8772.0
58	8269.0
59	7469.0
60	6669.0
61	6669.0
62	6039.5
63	5410.0
64	4575.0
65	3740.0
66	3159.0
67	2578.0
68	2578.0
69	2233.5
70	1889.0
71	1601.0
72	1313.0
73	1046.0
74	779.0
75	779.0
76	633.5
77	488.0
78	411.5
79	335.0
80	260.5
81	186.0
82	186.0
83	142.0
84	98.0
85	81.5
86	65.0
87	48.0
88	31.0
89	31.0
90	23.0
91	15.0
92	8.5
93	2.0
94	3.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	89892.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.577048013171364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.9508916067594	51.20700396030793
2	6.262720567640743	7.462288079028168
3	2.3769956119876765	4.248431451074623
4	1.251050322098777	2.9813554042628936
5	0.8869386611894314	2.642059360121034
6	0.5751097003080945	2.0558002936857562
7	0.4070581645037812	1.6975926667556624
8	0.33236859303519745	1.5841231700262537
9	0.2912893287274764	1.5618742490989186
>10	1.5105965829521053	16.25839896765007
>50	0.11390159648959014	4.635562675210252
>100	0.04107926430772103	3.6655097227784457
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	261	0.29034841810172207	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	222	0.24696302229341877	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	211	0.2347261157833845	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	200	0.22248920927335022	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	189	0.210252302763316	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	177	0.19690295020691495	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	174	0.19356561206781472	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	167	0.18577848974324745	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	152	0.16909179904774618	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	147	0.16352956881591243	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	143	0.15907978463044542	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	133	0.1479553241667779	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	131	0.1457304320740444	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	128	0.14239309393494415	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	118	0.13126863347127662	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	110	0.12236906510034262	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	109	0.12125661905397589	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	108	0.12014417300760913	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	106	0.11791928091487563	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	104	0.11569438882214213	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	103	0.11458194277577537	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	102	0.11346949672940863	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	95	0.10568237440484138	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	94	0.10456992835847462	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	94	0.10456992835847462	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	93	0.10345748231210786	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	91	0.10123259021937436	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	91	0.10123259021937436	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	91	0.10123259021937436	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	90	0.1001201441730076	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	90	0.1001201441730076	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	90	0.1001201441730076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0011124460463667512	0.0
15	0.0	0.0	0.0	0.0011124460463667512	0.0
16	0.0	0.0	0.0	0.0011124460463667512	0.0
17	0.0	0.0	0.0	0.0011124460463667512	0.0
18	0.0	0.0	0.0	0.0011124460463667512	0.0
19	0.0	0.0	0.0	0.0011124460463667512	0.0
20	0.0	0.0	0.0	0.0011124460463667512	0.0
21	0.0	0.0	0.0	0.0022248920927335023	0.0
22	0.0	0.0	0.0	0.0033373381391002535	0.0
23	0.0	0.0	0.0	0.0033373381391002535	0.0
24	0.0	0.0	0.0	0.004449784185467005	0.0
25	0.0	0.0	0.0	0.005562230231833756	0.0
26	0.0	0.0	0.0	0.006674676278200507	0.0
27	0.0	0.0	0.0	0.03226093534463578	0.0
28	0.0	0.0	0.0	0.09789525208027411	0.0
29	0.0	0.0	0.0	0.23361366973701775	0.0
30	0.0	0.0	0.0	0.3893561162283629	0.0
31	0.0	0.0	0.0	0.8921817291861345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	135	0.0	32.88889	1
ATCTAAG	40	5.8803278E-5	27.75	32
ATACGGA	65	9.191899E-8	25.615385	29
TAATACG	75	1.3382305E-8	24.666666	27
CATCTAA	45	1.309998E-4	24.666666	31
TCTAAGT	45	1.309998E-4	24.666666	33
AATACGG	75	1.3382305E-8	24.666666	28
TACGGAG	70	1.8822175E-7	23.785713	30
AAGTACC	40	0.0019174402	23.125	36
GCGGTAA	80	2.6557245E-8	23.125	23
CTAAGTA	40	0.0019174402	23.125	34
AGGGTGC	40	0.0019174402	23.125	35
TAAGTAC	40	0.0019174402	23.125	35
CTTATAC	75	3.6620077E-7	22.2	37
ACATCTA	50	2.675337E-4	22.199999	30
GAGGGTG	50	2.675337E-4	22.199999	34
CGCGGTA	85	5.0480594E-8	21.764706	22
GTAATAC	85	5.0480594E-8	21.764706	26
CGGTAAT	85	5.0480594E-8	21.764706	24
GTATCAA	205	0.0	21.658537	2
>>END_MODULE