FastQCFastQC Report
Fri 10 Feb 2017
ERR1631983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4104
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA230.560428849902534No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT160.3898635477582846No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA160.3898635477582846No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT140.341130604288499No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT130.31676413255360625No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA120.29239766081871343No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA110.2680311890838206No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA100.24366471734892786No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA100.24366471734892786No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT80.1949317738791423RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT80.1949317738791423No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA80.1949317738791423No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA80.1949317738791423No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC80.1949317738791423No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA80.1949317738791423No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT80.1949317738791423No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG80.1949317738791423No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG70.1705653021442495No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC70.1705653021442495No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC60.14619883040935672No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT60.14619883040935672No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT60.14619883040935672No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG60.14619883040935672No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC60.14619883040935672No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.14619883040935672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14619883040935672No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT60.14619883040935672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60.14619883040935672No Hit
CAACTAGACGCAGCCTGCAGGCAGCCCCACACCCGCCGCCTCC60.14619883040935672No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC60.14619883040935672No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG60.14619883040935672No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT60.14619883040935672No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG60.14619883040935672No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA60.14619883040935672No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA60.14619883040935672No Hit
GTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG50.12183235867446393No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.12183235867446393No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC50.12183235867446393No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA50.12183235867446393No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC50.12183235867446393No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC50.12183235867446393No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA50.12183235867446393No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCCTGTCTC50.12183235867446393No Hit
GGGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGA50.12183235867446393No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC50.12183235867446393No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC50.12183235867446393No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC50.12183235867446393No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG50.12183235867446393No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC50.12183235867446393No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT50.12183235867446393No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)