##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631983.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4104
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50487329434698	33.0	31.0	34.0	31.0	34.0
2	32.61647173489279	34.0	31.0	34.0	31.0	34.0
3	32.714424951267056	34.0	31.0	34.0	31.0	34.0
4	36.19663742690059	37.0	35.0	37.0	35.0	37.0
5	36.0916179337232	37.0	35.0	37.0	35.0	37.0
6	36.091861598440545	37.0	35.0	37.0	35.0	37.0
7	36.08308966861598	37.0	35.0	37.0	35.0	37.0
8	36.085038986354775	37.0	35.0	37.0	35.0	37.0
9	37.85526315789474	39.0	38.0	39.0	35.0	39.0
10	37.81578947368421	39.0	38.0	39.0	35.0	39.0
11	37.978070175438596	39.0	38.0	39.0	35.0	39.0
12	37.882066276803116	39.0	38.0	39.0	35.0	39.0
13	37.96491228070175	39.0	38.0	39.0	35.0	39.0
14	39.262670565302145	40.0	39.0	41.0	36.0	41.0
15	39.28216374269006	40.0	39.0	41.0	36.0	41.0
16	39.20029239766082	40.0	39.0	41.0	36.0	41.0
17	39.21052631578947	40.0	39.0	41.0	36.0	41.0
18	39.08211500974659	40.0	39.0	41.0	36.0	41.0
19	39.10526315789474	40.0	39.0	41.0	36.0	41.0
20	39.17519493177388	40.0	39.0	41.0	36.0	41.0
21	39.14303118908382	40.0	39.0	41.0	36.0	41.0
22	39.136939571150094	40.0	39.0	41.0	36.0	41.0
23	39.11622807017544	40.0	39.0	41.0	36.0	41.0
24	39.052875243664715	40.0	39.0	41.0	35.0	41.0
25	38.980506822612085	40.0	39.0	41.0	35.0	41.0
26	38.89035087719298	40.0	38.0	41.0	35.0	41.0
27	38.80141325536062	40.0	38.0	41.0	35.0	41.0
28	38.75438596491228	40.0	38.0	41.0	35.0	41.0
29	38.73586744639376	40.0	38.0	41.0	35.0	41.0
30	38.76072124756335	40.0	38.0	41.0	35.0	41.0
31	38.626461988304094	40.0	38.0	41.0	35.0	41.0
32	38.53460038986355	40.0	38.0	41.0	34.0	41.0
33	38.530701754385966	40.0	38.0	41.0	34.0	41.0
34	38.52363547758284	40.0	38.0	41.0	34.0	41.0
35	38.388888888888886	40.0	38.0	41.0	34.0	41.0
36	38.428606237816766	40.0	38.0	41.0	34.0	41.0
37	38.3182261208577	40.0	38.0	41.0	33.0	41.0
38	38.302875243664715	40.0	38.0	41.0	34.0	41.0
39	38.167397660818715	40.0	37.0	41.0	33.0	41.0
40	37.76900584795322	40.0	37.0	41.0	33.0	41.0
41	37.87573099415204	40.0	37.0	41.0	33.0	41.0
42	37.92446393762183	40.0	37.0	41.0	33.0	41.0
43	37.46028265107213	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	2.0
24	7.0
25	4.0
26	5.0
27	15.0
28	20.0
29	25.0
30	40.0
31	46.0
32	67.0
33	89.0
34	133.0
35	182.0
36	239.0
37	407.0
38	1000.0
39	1822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46978557504873	15.253411306042885	12.378167641325536	28.898635477582847
2	20.419103313840157	17.83625730994152	32.212475633528264	29.53216374269006
3	22.563352826510723	18.68908382066277	27.753411306042885	30.994152046783626
4	16.496101364522417	13.791423001949319	34.941520467836256	34.77095516569201
5	18.494152046783626	31.310916179337234	31.871345029239766	18.323586744639375
6	34.13742690058479	33.065302144249515	16.106237816764132	16.691033138401558
7	31.384015594541907	27.3635477582846	18.61598440545809	22.6364522417154
8	27.802144249512672	29.848927875243664	20.2729044834308	22.076023391812864
9	30.458089668615983	11.549707602339181	15.692007797270954	42.30019493177387
10	20.029239766081872	22.490253411306043	28.41130604288499	29.069200779727094
11	38.59649122807017	18.494152046783626	18.469785575048732	24.439571150097468
12	23.318713450292396	22.344054580896685	25.828460038986357	28.50877192982456
13	35.57504873294347	16.228070175438596	20.029239766081872	28.167641325536064
14	24.049707602339183	18.9083820662768	21.929824561403507	35.1120857699805
15	29.751461988304094	23.391812865497073	20.10233918128655	26.75438596491228
16	26.535087719298247	23.635477582846004	21.17446393762183	28.654970760233915
17	26.63255360623782	22.880116959064328	21.710526315789476	28.776803118908383
18	26.656920077972714	19.371345029239766	23.952241715399612	30.01949317738791
19	28.094541910331383	22.295321637426902	23.879142300194932	25.730994152046783
20	30.628654970760234	19.541910331384017	22.53898635477583	27.29044834307992
21	29.873294346978557	19.76120857699805	20.297270955165693	30.068226120857698
22	29.11793372319688	21.17446393762183	20.175438596491226	29.53216374269006
23	30.360623781676416	20.614035087719298	20.2729044834308	28.75243664717349
24	27.704678362573098	19.85867446393762	22.319688109161792	30.116959064327485
25	28.898635477582847	20.71150097465887	22.6364522417154	27.753411306042885
26	30.67738791423002	21.125730994152047	21.9541910331384	26.242690058479536
27	27.16861598440546	20.44346978557505	24.926900584795323	27.46101364522417
28	27.192982456140353	22.417153996101362	20.46783625730994	29.922027290448344
29	26.31578947368421	23.34307992202729	21.783625730994153	28.55750487329435
30	27.022417153996102	20.906432748538013	23.879142300194932	28.192007797270957
31	28.33820662768031	21.734892787524366	21.75925925925926	28.167641325536064
32	27.704678362573098	20.66276803118908	21.613060428849902	30.01949317738791
33	28.094541910331383	21.85672514619883	22.782651072124757	27.266081871345026
34	27.60721247563353	21.53996101364522	22.709551656920077	28.14327485380117
35	26.31578947368421	22.392787524366472	24.537037037037038	26.75438596491228
36	28.26510721247563	20.46783625730994	22.977582846003898	28.289473684210524
37	27.412280701754387	21.296296296296298	25.316764132553605	25.97465886939571
38	27.53411306042885	20.248538011695906	25.0	27.217348927875246
39	25.12183235867446	19.273879142300196	26.924951267056528	28.67933723196881
40	26.072124756335285	20.44346978557505	26.55945419103314	26.924951267056528
41	23.05068226120858	19.931773879142302	27.60721247563353	29.410331384015592
42	21.41812865497076	20.394736842105264	29.580896686159846	28.606237816764136
43	21.85672514619883	21.34502923976608	29.28849902534113	27.509746588693957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	2.0
10	1.5
11	1.0
12	1.0
13	3.0
14	5.0
15	7.5
16	10.0
17	10.0
18	10.0
19	10.0
20	8.5
21	7.0
22	7.5
23	8.0
24	6.0
25	4.0
26	4.0
27	9.0
28	14.0
29	12.5
30	11.0
31	17.0
32	23.0
33	23.0
34	24.5
35	26.0
36	37.5
37	49.0
38	52.0
39	55.0
40	55.0
41	78.0
42	101.0
43	133.0
44	165.0
45	207.0
46	249.0
47	249.0
48	295.5
49	342.0
50	377.0
51	412.0
52	437.5
53	463.0
54	463.0
55	441.5
56	420.0
57	386.0
58	352.0
59	339.0
60	326.0
61	326.0
62	333.0
63	340.0
64	255.0
65	170.0
66	152.5
67	135.0
68	135.0
69	122.0
70	109.0
71	94.5
72	80.0
73	96.5
74	113.0
75	113.0
76	92.0
77	71.0
78	46.0
79	21.0
80	13.0
81	5.0
82	5.0
83	4.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4104.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.53118908382066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.29046898638427	71.10136452241716
2	6.9288956127080175	11.159844054580896
3	2.3600605143721634	5.701754385964912
4	0.9077155824508321	2.923976608187134
5	0.45385779122541603	1.827485380116959
6	0.4841149773071104	2.3391812865497075
7	0.0605143721633888	0.341130604288499
8	0.2420574886535552	1.5594541910331383
9	0.0	0.0
>10	0.2723146747352496	3.0458089668615984
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	23	0.560428849902534	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	16	0.3898635477582846	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	16	0.3898635477582846	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	14	0.341130604288499	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	13	0.31676413255360625	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	12	0.29239766081871343	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	11	0.2680311890838206	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	10	0.24366471734892786	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	10	0.24366471734892786	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	8	0.1949317738791423	RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	8	0.1949317738791423	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	8	0.1949317738791423	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	8	0.1949317738791423	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	8	0.1949317738791423	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	8	0.1949317738791423	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	8	0.1949317738791423	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	8	0.1949317738791423	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	7	0.1705653021442495	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	7	0.1705653021442495	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	6	0.14619883040935672	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	6	0.14619883040935672	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	6	0.14619883040935672	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	6	0.14619883040935672	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	6	0.14619883040935672	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.14619883040935672	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14619883040935672	No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT	6	0.14619883040935672	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.14619883040935672	No Hit
CAACTAGACGCAGCCTGCAGGCAGCCCCACACCCGCCGCCTCC	6	0.14619883040935672	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	6	0.14619883040935672	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	6	0.14619883040935672	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	6	0.14619883040935672	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	6	0.14619883040935672	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	6	0.14619883040935672	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	6	0.14619883040935672	No Hit
GTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG	5	0.12183235867446393	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.12183235867446393	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	5	0.12183235867446393	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	5	0.12183235867446393	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	5	0.12183235867446393	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	5	0.12183235867446393	No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA	5	0.12183235867446393	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCCTGTCTC	5	0.12183235867446393	No Hit
GGGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGA	5	0.12183235867446393	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	5	0.12183235867446393	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	5	0.12183235867446393	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	5	0.12183235867446393	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	5	0.12183235867446393	No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC	5	0.12183235867446393	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	5	0.12183235867446393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.024366471734892786	0.0
25	0.0	0.0	0.0	0.024366471734892786	0.0
26	0.0	0.0	0.0	0.024366471734892786	0.0
27	0.0	0.0	0.0	0.07309941520467836	0.0
28	0.0	0.0	0.0	0.07309941520467836	0.0
29	0.0	0.0	0.0	0.24366471734892786	0.0
30	0.0	0.0	0.0	0.43859649122807015	0.0
31	0.0	0.0	0.0	0.804093567251462	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE