FastQCFastQC Report
Fri 10 Feb 2017
ERR1631982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708190
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17190.24273147036812157No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15850.2238099944930033No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14680.20728900436323586No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14630.20658297914401502No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13460.1900619890142476No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13460.1900619890142476No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10890.1537722927462969No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10890.1537722927462969No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10860.1533486776147644No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10840.15306626752707606No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10830.1529250624832319No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC10320.14572360524717942No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10220.14431155480873775No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG9730.13739250766037364No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG9240.1304734605120095No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC9150.129202615117412No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG8690.12270718310058036No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA8520.12030669735522953No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8440.1191770570044762No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7510.10604498792696875No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT7500.10590378288312459No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7460.10533896270774792No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7390.10435052740083875No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7240.10223245174317626No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7190.10152642652395544No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC7150.10096160634857877No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7130.10067919626089045No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7110.10039678617320212No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7110.10039678617320212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA250.005494937829.5999985
ATAATAT250.005494937829.5999983
TATATAC250.005494937829.5999983
CATAGTC250.005494937829.5999982
TCCTTAC250.005494937829.5999981
GGTATCA9500.027.6526321
GCTAAAC706.5829227E-926.428573
ATTACCT809.767973E-1025.437533
ATACGGC1200.024.66666829
TATACAC1850.024.037
GCCTAGT551.9013221E-523.5454541
TAAGGTA551.9013221E-523.5454545
GGGCTAA753.736568E-722.21
TATTAGA1107.4578566E-1121.8636362
GCGGTAA6650.021.14285723
TACGGCT1600.020.812530
CGTCTAT450.003824378820.55555727
ATCTAAT555.140415E-420.1818181
AGTATTA555.140415E-420.18181815
CAAATTA555.140415E-420.18181825
Produced by FastQC (version 0.11.5)