##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631982.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708190
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46033691523461	33.0	31.0	34.0	31.0	34.0
2	32.62867168415256	34.0	31.0	34.0	31.0	34.0
3	32.73661729196967	34.0	31.0	34.0	31.0	34.0
4	36.21369124105113	37.0	35.0	37.0	35.0	37.0
5	36.10578234654542	37.0	35.0	37.0	35.0	37.0
6	36.152593230630195	37.0	36.0	37.0	35.0	37.0
7	36.13746028608142	37.0	35.0	37.0	35.0	37.0
8	36.11473474632514	37.0	35.0	37.0	35.0	37.0
9	37.947220378711926	39.0	38.0	39.0	35.0	39.0
10	37.85978762761406	39.0	38.0	39.0	35.0	39.0
11	37.934864937375565	39.0	38.0	39.0	35.0	39.0
12	37.83923946963385	39.0	38.0	39.0	35.0	39.0
13	37.90914726274023	39.0	38.0	39.0	35.0	39.0
14	39.26539629195555	40.0	39.0	41.0	36.0	41.0
15	39.26658947457603	40.0	39.0	41.0	36.0	41.0
16	39.215278385743936	40.0	39.0	41.0	36.0	41.0
17	39.161277340826615	40.0	39.0	41.0	36.0	41.0
18	39.194774001327325	40.0	39.0	41.0	36.0	41.0
19	39.1906479899462	40.0	39.0	41.0	36.0	41.0
20	39.189122975472685	40.0	39.0	41.0	36.0	41.0
21	39.15440630339316	40.0	39.0	41.0	36.0	41.0
22	39.13210014261709	40.0	39.0	41.0	36.0	41.0
23	39.077397308631866	40.0	39.0	41.0	36.0	41.0
24	39.09280842711701	40.0	39.0	41.0	36.0	41.0
25	39.02645052881289	40.0	39.0	41.0	36.0	41.0
26	38.95841652663833	40.0	38.0	41.0	35.0	41.0
27	38.83508239314308	40.0	38.0	41.0	35.0	41.0
28	38.83747299453537	40.0	38.0	41.0	35.0	41.0
29	38.796851127522274	40.0	38.0	41.0	35.0	41.0
30	38.764722743896414	40.0	38.0	41.0	35.0	41.0
31	38.65141134441322	40.0	38.0	41.0	35.0	41.0
32	38.6171648851297	40.0	38.0	41.0	35.0	41.0
33	38.548093025882885	40.0	38.0	41.0	35.0	41.0
34	38.50295400951722	40.0	38.0	41.0	34.0	41.0
35	38.434691255171636	40.0	38.0	41.0	34.0	41.0
36	38.38223499343397	40.0	38.0	41.0	34.0	41.0
37	38.32358124232198	40.0	38.0	41.0	34.0	41.0
38	38.295848571710984	40.0	38.0	41.0	34.0	41.0
39	38.20312627967071	40.0	37.0	41.0	34.0	41.0
40	37.87184371425747	40.0	37.0	41.0	33.0	41.0
41	37.94377921179345	40.0	37.0	41.0	33.0	41.0
42	37.92545220915291	40.0	37.0	41.0	33.0	41.0
43	37.43043533515017	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	1.0
15	0.0
16	0.0
17	1.0
18	7.0
19	7.0
20	25.0
21	40.0
22	106.0
23	195.0
24	390.0
25	703.0
26	1135.0
27	1799.0
28	2689.0
29	4025.0
30	5579.0
31	7839.0
32	10580.0
33	14482.0
34	20274.0
35	29901.0
36	46880.0
37	84822.0
38	185438.0
39	291268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.985653567545434	16.012510766884596	11.882686849574267	28.119148815995707
2	21.13952470382242	19.17267964811703	31.78116042305031	27.90663522501024
3	21.739646140160126	18.79368531043929	28.36244510653921	31.10422344286138
4	15.054999364577304	15.598921193465031	33.69561840748951	35.65046103446815
5	17.459721261243452	32.387353676273314	32.87295782205341	17.279967240429826
6	35.626173766926954	32.90218726612915	15.436959008175771	16.034679958768127
7	31.446645674183483	26.846891370959767	19.25274290797667	22.453720046880076
8	27.839421624140414	29.780708566910008	20.22409240458069	22.155777404368884
9	28.429093887233652	11.861223682909953	17.14003304198026	42.56964938787613
10	19.178751465002332	22.901904856041458	29.469775060365155	28.449568618591055
11	40.29300046597665	18.901283553848543	17.8870077239159	22.918708256258913
12	22.84669368389839	23.260424462361794	25.816235755941204	28.076646097798612
13	35.99260085570257	16.894336265691408	20.091500868411018	27.021562010195005
14	24.825258758242843	19.308801310382805	22.42505542297971	33.44088450839464
15	29.374602860814186	23.65269207416089	19.86882051426877	27.103884550756153
16	27.067453649444357	22.84132789223231	21.990567503071208	28.10065095525212
17	27.439105324842206	22.92308561261808	20.978974568971605	28.658834493568108
18	26.96140866151739	20.522035047091883	22.814357728858077	29.702198562532654
19	29.402985074626862	22.117510837487114	22.092093929595165	26.38741015829085
20	30.924045806916222	20.174811844279077	21.679210381394824	27.221931967409873
21	27.71219587963682	21.823521936203562	20.935624620511444	29.528657563648174
22	29.52653948799051	21.932108614919724	20.306132535054154	28.235219362035615
23	27.70612406275152	21.683022917578615	21.599570736666713	29.011282283003148
24	28.317965517728293	21.24585210183708	21.709710670865164	28.726471709569466
25	28.429517502365186	21.300639658848613	22.408393227806098	27.861449610980106
26	28.641325068131433	22.22440305567715	20.8811194735876	28.25315240260382
27	28.07184512630791	21.294144226831783	22.605797879100244	28.028212767760063
28	26.835453762408395	22.755334020531212	21.25644248012539	29.152769736935003
29	28.27871051553961	23.32100142617094	21.614962086445725	26.785325971843715
30	27.993335121930556	20.72593513040286	22.344286137900845	28.93644360976574
31	27.49996469873904	21.643485505302248	22.241347660938448	28.61520213502026
32	25.94727403662859	21.998616190570328	22.172439599542496	29.88167017325859
33	26.64073200694729	21.083466301416287	23.71171578248775	28.564085909148673
34	27.39942670752199	21.46895607111086	23.364774989762633	27.76684223160451
35	26.853810418108136	22.478572134596646	23.155650319829423	27.51196712746579
36	26.34603708044451	21.44495121365735	23.59606885157938	28.612942854318757
37	26.275999378697808	21.433513605105976	24.819469351445235	27.471017664750985
38	26.00601533486776	20.43660599556616	24.576455470989426	28.98092319857665
39	25.2583346277129	20.21660853725695	26.681681469662095	27.84337536536805
40	26.041457800872646	20.404411245569694	26.547819088097828	27.006311865459836
41	23.442155353789236	20.094466174331746	27.78703455287423	28.676343919004786
42	21.641367429644585	21.11848515228964	28.819667038506612	28.42048037955916
43	21.63670766319773	20.794560781711123	28.131998474985526	29.436733080105622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	6.5
4	12.0
5	12.0
6	15.5
7	19.0
8	27.5
9	36.0
10	49.5
11	63.0
12	63.0
13	124.5
14	186.0
15	381.5
16	577.0
17	567.5
18	558.0
19	558.0
20	657.0
21	756.0
22	679.0
23	602.0
24	658.5
25	715.0
26	715.0
27	847.0
28	979.0
29	1352.5
30	1726.0
31	2408.5
32	3091.0
33	3091.0
34	5096.0
35	7101.0
36	8013.5
37	8926.0
38	11188.5
39	13451.0
40	13451.0
41	16259.5
42	19068.0
43	23967.5
44	28867.0
45	38306.5
46	47746.0
47	47746.0
48	53390.5
49	59035.0
50	67366.0
51	75697.0
52	79082.0
53	82467.0
54	82467.0
55	76290.0
56	70113.0
57	65076.0
58	60039.0
59	59829.5
60	59620.0
61	59620.0
62	55436.5
63	51253.0
64	41096.0
65	30939.0
66	28375.5
67	25812.0
68	25812.0
69	22861.0
70	19910.0
71	19307.0
72	18704.0
73	15236.0
74	11768.0
75	11768.0
76	7659.5
77	3551.0
78	2830.0
79	2109.0
80	1677.0
81	1245.0
82	1245.0
83	992.5
84	740.0
85	563.5
86	387.0
87	288.0
88	189.0
89	189.0
90	140.0
91	91.0
92	63.5
93	36.0
94	20.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	708190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.29591419090634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.7307746433895	35.40330713193342
2	6.43128285108695	5.311714095118683
3	2.1815880277620665	2.702720159831127
4	1.131493082277563	1.8690416493335347
5	0.7431866978938881	1.5345287052024517
6	0.5215639324052949	1.2923075638608439
7	0.36051669216626536	1.0421506468861241
8	0.2997876423345439	0.9904003802673155
9	0.24326723642811462	0.9041348628894911
>10	1.8445675551074525	15.483155222402731
>50	0.2585715353603125	7.4899397943475385
>100	0.22719818829436242	17.987786287062736
>500	0.021720008896122658	5.652193755066689
>1k	0.004481906597612612	2.3366197457973423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1719	0.24273147036812157	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1585	0.2238099944930033	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1468	0.20728900436323586	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1463	0.20658297914401502	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1346	0.1900619890142476	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1346	0.1900619890142476	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1089	0.1537722927462969	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1089	0.1537722927462969	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1086	0.1533486776147644	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1084	0.15306626752707606	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1083	0.1529250624832319	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1032	0.14572360524717942	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1022	0.14431155480873775	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	973	0.13739250766037364	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	924	0.1304734605120095	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	915	0.129202615117412	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	869	0.12270718310058036	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	852	0.12030669735522953	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	844	0.1191770570044762	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	751	0.10604498792696875	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	750	0.10590378288312459	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	746	0.10533896270774792	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	739	0.10435052740083875	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	724	0.10223245174317626	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	719	0.10152642652395544	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	715	0.10096160634857877	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	713	0.10067919626089045	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	711	0.10039678617320212	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	711	0.10039678617320212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.412050438441661E-4	0.0	0.0	0.0	0.0
6	1.412050438441661E-4	0.0	0.0	0.0	0.0
7	1.412050438441661E-4	0.0	0.0	0.0	0.0
8	1.412050438441661E-4	0.0	0.0	0.0	0.0
9	1.412050438441661E-4	0.0	0.0	0.0	0.0
10	2.824100876883322E-4	0.0	0.0	0.0	0.0
11	4.2361513153249834E-4	0.0	0.0	0.0	0.0
12	4.2361513153249834E-4	0.0	0.0	0.0	0.0
13	4.2361513153249834E-4	0.0	0.0	0.0	0.0
14	4.2361513153249834E-4	0.0	0.0	0.0	0.0
15	5.648201753766644E-4	0.0	0.0	1.412050438441661E-4	0.0
16	5.648201753766644E-4	0.0	0.0	2.824100876883322E-4	0.0
17	7.060252192208306E-4	0.0	0.0	4.2361513153249834E-4	0.0
18	8.472302630649967E-4	0.0	0.0	4.2361513153249834E-4	0.0
19	8.472302630649967E-4	0.0	0.0	7.060252192208306E-4	0.0
20	8.472302630649967E-4	0.0	0.0	0.001270845394597495	0.0
21	8.472302630649967E-4	0.0	0.0	0.002118075657662492	0.0
22	8.472302630649967E-4	0.0	0.0	0.0032477160084158204	0.0
23	8.472302630649967E-4	0.0	0.0	0.004800971490701648	0.0
24	8.472302630649967E-4	0.0	0.0	0.0067778421045199735	0.0
25	8.472302630649967E-4	0.0	0.0	0.008048687499117468	0.0
26	8.472302630649967E-4	0.0	0.0	0.011155198463689123	0.0
27	9.884353069091628E-4	0.0	0.0	0.03177113486493738	0.0
28	9.884353069091628E-4	1.412050438441661E-4	0.0	0.128355384854347	0.0
29	9.884353069091628E-4	1.412050438441661E-4	0.0	0.28495177847752723	0.0
30	9.884353069091628E-4	1.412050438441661E-4	0.0	0.47346051200948897	0.0
31	9.884353069091628E-4	1.412050438441661E-4	0.0	1.0563549329982067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTA	25	0.0054949378	29.599998	5
ATAATAT	25	0.0054949378	29.599998	3
TATATAC	25	0.0054949378	29.599998	3
CATAGTC	25	0.0054949378	29.599998	2
TCCTTAC	25	0.0054949378	29.599998	1
GGTATCA	950	0.0	27.652632	1
GCTAAAC	70	6.5829227E-9	26.42857	3
ATTACCT	80	9.767973E-10	25.4375	33
ATACGGC	120	0.0	24.666668	29
TATACAC	185	0.0	24.0	37
GCCTAGT	55	1.9013221E-5	23.545454	1
TAAGGTA	55	1.9013221E-5	23.545454	5
GGGCTAA	75	3.736568E-7	22.2	1
TATTAGA	110	7.4578566E-11	21.863636	2
GCGGTAA	665	0.0	21.142857	23
TACGGCT	160	0.0	20.8125	30
CGTCTAT	45	0.0038243788	20.555557	27
ATCTAAT	55	5.140415E-4	20.181818	1
AGTATTA	55	5.140415E-4	20.181818	15
CAAATTA	55	5.140415E-4	20.181818	25
>>END_MODULE