FastQCFastQC Report
Fri 10 Feb 2017
ERR1631981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47233
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1170.24770817013528676No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA910.19266191010522304No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA900.1905447462579129No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA840.17784176317405204No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC730.15455296085364045No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG730.15455296085364045No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA720.1524357970063303No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT710.1503186331590202No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT640.13549848622784916No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA610.12914699468591873No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT550.11644401160205789No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA540.11432684775474775No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA540.11432684775474775No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC520.11009252006012746No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA500.10585819236550717No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC490.10374102851819701No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT480.10162386467088688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGGT200.00182411437.027
TCGGGTT200.00182411437.028
GGTATCA551.7773345E-830.2727281
GGTTGTA250.00544376929.59999831
GGCATCC250.00544376929.59999835
CGGGTTG250.00544376929.59999829
CCTTCGG250.00544376929.59999825
GCTCACA358.7434956E-426.4285731
GCGGTAA551.8530545E-523.54545423
GGTAATA603.629819E-521.58333225
GCAGCCG704.9331484E-621.14285717
ACCACGT450.003772523720.5555575
AGCATGT450.003772523720.55555737
ACTCATC555.043392E-420.18181822
CCAGCAG656.724696E-519.92307714
CGCGGTA656.724696E-519.92307722
CCGCCCT701.1880763E-418.515
GTATCAA902.0647367E-618.52
GGATACC609.0592075E-418.51
GCCAGCA701.1880763E-418.513