##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631981.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47233
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57569919336058	34.0	31.0	34.0	31.0	34.0
2	32.73556623547096	34.0	31.0	34.0	31.0	34.0
3	32.8416361442212	34.0	31.0	34.0	31.0	34.0
4	36.280799441068744	37.0	37.0	37.0	35.0	37.0
5	36.180361188152354	37.0	35.0	37.0	35.0	37.0
6	36.21995215209705	37.0	36.0	37.0	35.0	37.0
7	36.2275527703089	37.0	36.0	37.0	35.0	37.0
8	36.211652869815595	37.0	36.0	37.0	35.0	37.0
9	38.029936696800966	39.0	38.0	39.0	35.0	39.0
10	37.9690682361908	39.0	38.0	39.0	35.0	39.0
11	38.05578726737662	39.0	38.0	39.0	35.0	39.0
12	37.978786018249956	39.0	38.0	39.0	35.0	39.0
13	38.041813985984376	39.0	38.0	39.0	35.0	39.0
14	39.41009463722398	40.0	39.0	41.0	37.0	41.0
15	39.40211292951962	40.0	39.0	41.0	37.0	41.0
16	39.34859102745961	40.0	39.0	41.0	36.0	41.0
17	39.31842144263545	40.0	39.0	41.0	36.0	41.0
18	39.33180191815045	40.0	39.0	41.0	36.0	41.0
19	39.363876950437195	40.0	39.0	41.0	37.0	41.0
20	39.34713018440497	40.0	39.0	41.0	36.0	41.0
21	39.31427180149472	40.0	39.0	41.0	36.0	41.0
22	39.284779709101684	40.0	39.0	41.0	36.0	41.0
23	39.216988122710816	40.0	39.0	41.0	36.0	41.0
24	39.23278216501175	40.0	39.0	41.0	36.0	41.0
25	39.19297948468232	40.0	39.0	41.0	36.0	41.0
26	39.098109372684355	40.0	39.0	41.0	36.0	41.0
27	38.98731818855461	40.0	39.0	41.0	35.0	41.0
28	38.987275845277665	40.0	39.0	41.0	35.0	41.0
29	38.95450214892131	40.0	38.0	41.0	35.0	41.0
30	38.90957593208138	40.0	38.0	41.0	35.0	41.0
31	38.833823809624626	40.0	38.0	41.0	35.0	41.0
32	38.78786018249952	40.0	38.0	41.0	35.0	41.0
33	38.72004742447018	40.0	38.0	41.0	35.0	41.0
34	38.687718332521754	40.0	38.0	41.0	35.0	41.0
35	38.61514195583596	40.0	38.0	41.0	35.0	41.0
36	38.57713886477674	40.0	38.0	41.0	35.0	41.0
37	38.54404759384329	40.0	38.0	41.0	34.0	41.0
38	38.48898016217475	40.0	38.0	41.0	34.0	41.0
39	38.42842927614168	40.0	38.0	41.0	34.0	41.0
40	38.118222429233796	40.0	37.0	41.0	34.0	41.0
41	38.18440497110071	40.0	37.0	41.0	34.0	41.0
42	38.18438379946224	40.0	37.0	41.0	34.0	41.0
43	37.70160692736011	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	3.0
22	5.0
23	15.0
24	27.0
25	36.0
26	69.0
27	94.0
28	150.0
29	219.0
30	329.0
31	429.0
32	647.0
33	865.0
34	1256.0
35	1921.0
36	2965.0
37	5026.0
38	11396.0
39	21779.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.60510659919971	15.897783329451867	12.000084686553892	28.497025384794526
2	22.103190565917895	18.711494082527047	31.2048779455042	27.980437406050857
3	21.114475049224062	19.01848284038702	28.50125971248915	31.36578239789977
4	15.8215654309487	15.537865475409141	33.42578282133255	35.21478627230961
5	17.784176317405205	32.329091948425884	32.843562763322254	17.043168970846654
6	35.87110706497576	32.95577244722969	14.841318569644105	16.331801918150447
7	31.2048779455042	26.89644951622806	19.372049202887812	22.526623335379924
8	28.47161941862681	29.852010247073018	19.702326763068193	21.974043571231977
9	28.23873139542269	12.273198822856902	17.519530836491437	41.96853894522897
10	19.93944911396693	22.947938940994643	29.136408866682196	27.97620307835623
11	40.478055596722626	18.88721868185379	18.04670463447166	22.58802108695192
12	23.551330637478035	22.71928524548515	26.371392882095147	27.35799123494167
13	35.94944212732623	17.31416594330235	20.017784176317406	26.71860775305401
14	24.50828869646222	18.912624648021513	22.34243008066394	34.236656574852326
15	30.163233332627616	23.094023246459045	19.253488027438443	27.4892553934749
16	26.4031503398048	23.21470158575572	21.396057840916306	28.98609023352317
17	27.993140389134712	22.69387927931743	20.938750449897316	28.374229881650542
18	27.855524739059558	20.648699002815828	22.492748713822962	29.003027544301652
19	29.20204094594881	21.381237693985135	22.403827832235937	27.012893527830116
20	30.32413778502318	20.409459488069782	21.931700294285775	27.33470243262126
21	28.389050028581714	21.148349670781023	21.336777253191624	29.125823047445643
22	28.920458154256558	22.128596532085616	20.54072364660301	28.410221667054813
23	29.2422670590477	21.533673490991468	21.184341456185294	28.039717993775536
24	28.374229881650542	21.042491478415513	21.732686892638622	28.850591747295322
25	29.225329748269218	21.044608642262823	22.137065187474857	27.5929964219931
26	28.740499227235194	21.910528655812673	21.56754811254843	27.7814240044037
27	27.855524739059558	21.027671331484342	23.33326276120509	27.783541168251013
28	27.31776512184278	22.338195752969323	21.419346643236718	28.92469248195118
29	28.10323290919484	22.51392035229606	21.927465966591157	27.455380771917937
30	27.88304786907459	21.258442190841148	23.06650011644401	27.79200982364025
31	27.95926576757775	21.87665403425571	21.9782778989266	28.18580229923994
32	26.392564520568246	21.544259310228018	22.215400249825336	29.8477759193784
33	26.69743611458091	21.175872800796053	23.786335824529463	28.34035526009358
34	27.36857705417822	20.928164630660767	23.17659263650414	28.52666567865687
35	26.574640611436916	22.547794973853026	23.794804479918703	27.082759934791355
36	26.693201786886288	21.618360044883875	24.434187961806366	27.254250206423475
37	26.71860775305401	21.25420786314653	24.22247157707535	27.804712806724112
38	25.85268773950416	20.576715432007283	25.54569898164419	28.024897846844365
39	25.382677365401307	19.647280503038132	26.69743611458091	28.27260601697966
40	25.060868460610163	20.55342662968687	27.497724048864143	26.887980860838823
41	23.813858954544493	19.856879723921832	28.18156797154532	28.147693349988355
42	21.908411491965364	20.458154256557915	29.27190735291004	28.36152689856668
43	21.4151123155421	20.756674359028647	28.685452967205133	29.142760358224123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.5
7	1.0
8	3.0
9	5.0
10	5.0
11	5.0
12	5.0
13	11.5
14	18.0
15	30.5
16	43.0
17	46.5
18	50.0
19	50.0
20	56.5
21	63.0
22	53.5
23	44.0
24	57.5
25	71.0
26	71.0
27	76.0
28	81.0
29	114.5
30	148.0
31	206.0
32	264.0
33	264.0
34	353.0
35	442.0
36	516.5
37	591.0
38	736.5
39	882.0
40	882.0
41	1104.0
42	1326.0
43	1558.5
44	1791.0
45	2396.0
46	3001.0
47	3001.0
48	3423.5
49	3846.0
50	4381.5
51	4917.0
52	5053.0
53	5189.0
54	5189.0
55	4971.0
56	4753.0
57	4712.5
58	4672.0
59	4352.0
60	4032.0
61	4032.0
62	3702.5
63	3373.0
64	2819.5
65	2266.0
66	2025.0
67	1784.0
68	1784.0
69	1543.5
70	1303.0
71	1075.5
72	848.0
73	701.0
74	554.0
75	554.0
76	457.0
77	360.0
78	281.5
79	203.0
80	174.0
81	145.0
82	145.0
83	112.0
84	79.0
85	59.5
86	40.0
87	31.5
88	23.0
89	23.0
90	18.0
91	13.0
92	8.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	47233.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.25458895263904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.98326065550711	68.46907882201003
2	4.461949641299761	6.715643723667775
3	1.513574342382895	3.417102449558571
4	0.8018005345336896	2.4135667859335634
5	0.5682937121958082	2.138335485783245
6	0.3150935433956956	1.4227341053924163
7	0.2869601913067942	1.5116549869794424
8	0.17442678295118863	1.0501132682658312
9	0.12660008440005627	0.857451358160608
>10	0.728653819102546	9.863866364617959
>50	0.03657335771557181	1.8927444794952681
>100	0.0028133352088901393	0.24770817013528676
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	117	0.24770817013528676	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	91	0.19266191010522304	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	90	0.1905447462579129	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	84	0.17784176317405204	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	73	0.15455296085364045	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	73	0.15455296085364045	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	72	0.1524357970063303	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	71	0.1503186331590202	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	64	0.13549848622784916	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	61	0.12914699468591873	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	55	0.11644401160205789	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	54	0.11432684775474775	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	54	0.11432684775474775	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	52	0.11009252006012746	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	50	0.10585819236550717	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	49	0.10374102851819701	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	48	0.10162386467088688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0021171638473101435	0.0
20	0.0	0.0	0.0	0.004234327694620287	0.0
21	0.0	0.0	0.0	0.008468655389240574	0.0
22	0.0	0.0	0.0	0.01270298308386086	0.0
23	0.0	0.0	0.0	0.01270298308386086	0.0
24	0.0	0.0	0.0	0.01270298308386086	0.0
25	0.0	0.0	0.0	0.01270298308386086	0.0
26	0.0	0.0	0.0	0.014820146931171002	0.0
27	0.0	0.0	0.0	0.029640293862342005	0.0
28	0.0	0.0	0.0	0.11009252006012746	0.0
29	0.0	0.0	0.0	0.24770817013528676	0.0
30	0.0	0.0	0.0	0.44672157178244026	0.0
31	0.0	0.0	0.0	0.9569580589841847	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGGT	20	0.001824114	37.0	27
TCGGGTT	20	0.001824114	37.0	28
GGTATCA	55	1.7773345E-8	30.272728	1
GGTTGTA	25	0.005443769	29.599998	31
GGCATCC	25	0.005443769	29.599998	35
CGGGTTG	25	0.005443769	29.599998	29
CCTTCGG	25	0.005443769	29.599998	25
GCTCACA	35	8.7434956E-4	26.42857	31
GCGGTAA	55	1.8530545E-5	23.545454	23
GGTAATA	60	3.629819E-5	21.583332	25
GCAGCCG	70	4.9331484E-6	21.142857	17
ACCACGT	45	0.0037725237	20.555557	5
AGCATGT	45	0.0037725237	20.555557	37
ACTCATC	55	5.043392E-4	20.181818	22
CCAGCAG	65	6.724696E-5	19.923077	14
CGCGGTA	65	6.724696E-5	19.923077	22
CCGCCCT	70	1.1880763E-4	18.5	15
GTATCAA	90	2.0647367E-6	18.5	2
GGATACC	60	9.0592075E-4	18.5	1
GCCAGCA	70	1.1880763E-4	18.5	13
>>END_MODULE