FastQCFastQC Report
Fri 10 Feb 2017
ERR1631980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792852
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC24670.311155171456968No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT16840.21239777411168792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.17619934111284327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12020.15160458698470836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11210.14138830450071388No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT10800.13621709978659322No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG10110.12751434063356087No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9960.12562243646985818No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9750.12297377064067444No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9630.12146024730971229No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9410.11868545453628168No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT9230.11641516953983845No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG9120.11502777315312315No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG8660.10922593371776826No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC8460.10670339483283134No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC8210.1035502212266602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.030.574851
ATACGGC1005.329639E-1022.19999929
TACGGCT1107.4578566E-1121.86363630
CCAGGAC7250.021.6896553
CGGGATA603.72441E-521.58333216
CTTATAC10800.021.06944537
GCGGTAA4850.020.59793923
CTATCCG909.468931E-820.5555579
ACCGTCG450.003824775820.55555717
CATCAGA7250.020.41379416
ACAGGCT7250.020.158628
GACAGGC7450.020.1140947
GATATTG656.898679E-519.9230777
TTACTCG759.259826E-619.73333429
TATCCGG759.259826E-619.73333410
TATACAC3100.019.69354837
AACTCCG5000.019.245
AGGCCAT7700.019.2207825
GGACAGG8250.019.0606066
TATTAGA1750.019.028572
Produced by FastQC (version 0.11.5)