##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631980.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792852
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58937733650164	34.0	31.0	34.0	31.0	34.0
2	32.75865104710589	34.0	31.0	34.0	31.0	34.0
3	32.85398662045375	34.0	31.0	34.0	31.0	34.0
4	36.29112369017168	37.0	37.0	37.0	35.0	37.0
5	36.2096330714938	37.0	35.0	37.0	35.0	37.0
6	36.25128271102299	37.0	37.0	37.0	35.0	37.0
7	36.238256320221176	37.0	37.0	37.0	35.0	37.0
8	36.21709852532377	37.0	37.0	37.0	35.0	37.0
9	38.046702284915725	39.0	38.0	39.0	35.0	39.0
10	37.98843920429034	39.0	38.0	39.0	35.0	39.0
11	38.05891137311882	39.0	38.0	39.0	35.0	39.0
12	38.00449264175407	39.0	38.0	39.0	35.0	39.0
13	38.04301559433539	39.0	38.0	39.0	35.0	39.0
14	39.442122867824004	40.0	39.0	41.0	37.0	41.0
15	39.440341955371245	40.0	39.0	41.0	37.0	41.0
16	39.40539344038989	40.0	39.0	41.0	37.0	41.0
17	39.36975248848461	40.0	39.0	41.0	36.0	41.0
18	39.36208144773552	40.0	39.0	41.0	36.0	41.0
19	39.3883763930721	41.0	39.0	41.0	36.0	41.0
20	39.36547047872743	40.0	39.0	41.0	36.0	41.0
21	39.345807288119346	40.0	39.0	41.0	36.0	41.0
22	39.313247617462025	40.0	39.0	41.0	36.0	41.0
23	39.252536412848805	40.0	39.0	41.0	36.0	41.0
24	39.263925675914294	40.0	39.0	41.0	36.0	41.0
25	39.205501152800274	40.0	39.0	41.0	36.0	41.0
26	39.14307714428418	40.0	39.0	41.0	36.0	41.0
27	39.046641743982484	40.0	39.0	41.0	35.0	41.0
28	39.0308304702517	40.0	39.0	41.0	35.0	41.0
29	39.0037510153219	40.0	39.0	41.0	35.0	41.0
30	38.95791017743539	40.0	38.0	41.0	35.0	41.0
31	38.8918928122777	40.0	38.0	41.0	35.0	41.0
32	38.844828543031994	40.0	38.0	41.0	35.0	41.0
33	38.7799425365642	40.0	38.0	41.0	35.0	41.0
34	38.731270148779345	40.0	38.0	41.0	35.0	41.0
35	38.6509272852941	40.0	38.0	41.0	35.0	41.0
36	38.614283624182065	40.0	38.0	41.0	35.0	41.0
37	38.570419195511896	40.0	38.0	41.0	35.0	41.0
38	38.53901106385555	40.0	38.0	41.0	35.0	41.0
39	38.475911014918296	40.0	38.0	41.0	34.0	41.0
40	38.15272963932739	40.0	38.0	41.0	34.0	41.0
41	38.21085019650578	40.0	37.0	41.0	34.0	41.0
42	38.19235368013198	40.0	37.0	41.0	34.0	41.0
43	37.7238677584215	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	5.0
18	2.0
19	4.0
20	30.0
21	43.0
22	95.0
23	225.0
24	395.0
25	620.0
26	1081.0
27	1713.0
28	2580.0
29	3945.0
30	5412.0
31	7324.0
32	10423.0
33	13765.0
34	20393.0
35	32179.0
36	47438.0
37	83122.0
38	184266.0
39	377788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45993955996832	16.23468188262122	12.203034109770803	28.10234444763966
2	20.063391402178464	18.572697048124997	34.52548521035452	26.83842633934202
3	21.010478626528027	20.262545847144235	28.609500890456225	30.11747463587151
4	15.237396134461411	15.232477183635787	33.60324499402158	35.92688168788122
5	16.884235645492478	33.55317259715559	32.7708071619924	16.791784595359537
6	35.33496793853077	33.565028529914784	15.051611145585808	16.04839238596863
7	31.19863480195547	27.803045208941896	19.787047267333627	21.211272721769006
8	27.48533143638409	32.17233481154112	19.253278039280975	21.089055712793815
9	28.37856245554025	13.319257566355386	17.36137387557829	40.940806102526075
10	19.645154455055923	23.773919974976415	29.594047817247105	26.986877752720556
11	38.34322168576229	20.231140238026768	19.406396149596645	22.0192419266143
12	24.23466170231014	23.285682573796876	26.57292912170241	25.90672660219057
13	33.0247007007613	17.739250200541843	21.97295333807571	27.263095760621148
14	23.85602861568111	19.086159838153904	23.85754213901207	33.20026940715291
15	28.679122963680488	23.853001569019185	21.093470155842454	26.374405311457878
16	25.735698465791852	23.74327112752443	21.970304672246524	28.5507257344372
17	26.021375994510954	24.117868151937564	22.755571027127385	27.1051848264241
18	26.097430541891804	21.90597993068063	24.093399524753675	27.903190002673888
19	27.936235262066567	22.17992765358478	23.39692653862259	26.48691054572606
20	27.638323419755515	21.987584063608338	23.65208134683396	26.722011169802183
21	28.268958140989742	22.318541165312062	22.43621760429437	26.976283089403825
22	28.00510057362534	23.587125970546836	21.754375343695923	26.6533981121319
23	27.579422136792235	23.0967444113151	22.523119068880444	26.800714383012213
24	27.892721466301403	22.389676761867282	22.423731036813933	27.29387073501738
25	27.4070066040068	22.270865180386757	23.552188806990458	26.769939408615983
26	27.775675662040328	22.788994667352796	23.36615156422636	26.06917810638051
27	27.979749057831725	22.417298562657344	23.010851962283	26.592100417227932
28	26.368351218134027	22.671065974481998	23.42946729023828	27.5311155171457
29	27.115905616685083	23.04515849111814	23.30031329932951	26.538622592867267
30	26.334801450964367	22.352469313314465	24.526014943520355	26.786714292200813
31	26.62098348746046	22.53787592135733	23.525576021754375	27.315564569427835
32	24.968594390882533	22.414523769883914	24.231634655648218	28.38524718358533
33	25.89890673164727	22.700327425547265	24.721133326270227	26.67963251653524
34	26.241719766110194	22.47418181451267	24.74497131873288	26.539127100644254
35	26.342369067619174	22.91512161159964	24.73740370207807	26.005105618703112
36	26.274008263837388	22.07360263958469	24.41653675591409	27.23585234066383
37	25.155640649200606	21.7292760817908	25.075171658771122	28.03991161023747
38	25.62205808902544	20.84071175957177	26.18180946759294	27.35542068380984
39	24.034876622623138	20.795053805754414	27.36677210879206	27.80329746283039
40	24.35145525268272	20.846891979839867	28.355228970854583	26.446423796622824
41	22.937446080731334	20.271122479353018	29.147810688501764	27.643620751413884
42	21.748699631204815	21.517256688511853	29.163450429588373	27.57059325069496
43	21.44574271112389	20.55213331113499	29.453668528300366	28.548455449440752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	21.5
2	17.0
3	50.5
4	84.0
5	84.0
6	103.0
7	122.0
8	134.5
9	147.0
10	209.5
11	272.0
12	272.0
13	542.0
14	812.0
15	1614.0
16	2416.0
17	2379.0
18	2342.0
19	2342.0
20	2549.5
21	2757.0
22	2311.0
23	1865.0
24	2139.0
25	2413.0
26	2413.0
27	2860.0
28	3307.0
29	4351.5
30	5396.0
31	6924.0
32	8452.0
33	8452.0
34	11464.5
35	14477.0
36	16669.5
37	18862.0
38	22474.5
39	26087.0
40	26087.0
41	30057.5
42	34028.0
43	39274.5
44	44521.0
45	49785.5
46	55050.0
47	55050.0
48	58757.5
49	62465.0
50	68477.0
51	74489.0
52	76379.0
53	78269.0
54	78269.0
55	75466.5
56	72664.0
57	66473.0
58	60282.0
59	55656.5
60	51031.0
61	51031.0
62	49065.0
63	47099.0
64	38121.0
65	29143.0
66	25911.5
67	22680.0
68	22680.0
69	20002.5
70	17325.0
71	16200.0
72	15075.0
73	16637.0
74	18199.0
75	18199.0
76	15672.0
77	13145.0
78	8850.0
79	4555.0
80	2944.5
81	1334.0
82	1334.0
83	1070.5
84	807.0
85	629.0
86	451.0
87	344.5
88	238.0
89	238.0
90	175.0
91	112.0
92	71.5
93	31.0
94	17.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	792852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.8867091702906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.79462311260178	38.51038296030048
2	6.389307686865528	5.735899918796703
3	2.2105764432959494	2.9767650572676203
4	1.1570668741697365	2.0774769708573677
5	0.782888437236293	1.7570642797504397
6	0.522908333359995	1.4082980569350876
7	0.3964821639628586	1.2457745709505816
8	0.3056480239488508	1.0975627167572868
9	0.24640466540244124	0.9954265098709933
>10	1.7845360375076231	15.822000318762047
>50	0.21113873512995443	6.5552115015297145
>100	0.18400439561806883	16.89982636134047
>500	0.012436549012586933	3.654414270682729
>1k	0.0019785418883661032	1.2638965061984915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2467	0.311155171456968	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1684	0.21239777411168792	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1397	0.17619934111284327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1202	0.15160458698470836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1121	0.14138830450071388	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1080	0.13621709978659322	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1011	0.12751434063356087	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	996	0.12562243646985818	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	975	0.12297377064067444	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	963	0.12146024730971229	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	941	0.11868545453628168	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	923	0.11641516953983845	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	912	0.11502777315312315	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	866	0.10922593371776826	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	846	0.10670339483283134	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	821	0.1035502212266602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2612694424684557E-4
2	0.0	0.0	0.0	0.0	1.2612694424684557E-4
3	0.0	0.0	0.0	0.0	1.2612694424684557E-4
4	0.0	0.0	0.0	0.0	1.2612694424684557E-4
5	0.0	0.0	0.0	0.0	1.2612694424684557E-4
6	0.0	0.0	0.0	0.0	1.2612694424684557E-4
7	0.0	0.0	0.0	0.0	1.2612694424684557E-4
8	1.2612694424684557E-4	0.0	0.0	0.0	1.2612694424684557E-4
9	1.2612694424684557E-4	0.0	0.0	0.0	1.2612694424684557E-4
10	1.2612694424684557E-4	0.0	0.0	1.2612694424684557E-4	1.2612694424684557E-4
11	1.2612694424684557E-4	0.0	0.0	2.5225388849369114E-4	1.2612694424684557E-4
12	1.2612694424684557E-4	0.0	0.0	3.783808327405367E-4	1.2612694424684557E-4
13	1.2612694424684557E-4	0.0	0.0	5.045077769873823E-4	1.2612694424684557E-4
14	1.2612694424684557E-4	0.0	0.0	0.00113514249822161	1.2612694424684557E-4
15	2.5225388849369114E-4	0.0	0.0	0.0013873963867153012	1.2612694424684557E-4
16	2.5225388849369114E-4	0.0	0.0	0.002018031107949529	2.5225388849369114E-4
17	3.783808327405367E-4	0.0	0.0	0.0023964119406900655	2.5225388849369114E-4
18	3.783808327405367E-4	0.0	0.0	0.002900919717677448	2.5225388849369114E-4
19	5.045077769873823E-4	0.0	0.0	0.0031531736061711393	2.5225388849369114E-4
20	5.045077769873823E-4	0.0	0.0	0.003909935271652213	3.783808327405367E-4
21	5.045077769873823E-4	0.0	0.0	0.006684728045082815	3.783808327405367E-4
22	5.045077769873823E-4	0.0	0.0	0.009333393874266571	3.783808327405367E-4
23	5.045077769873823E-4	0.0	0.0	0.011855932759203483	3.783808327405367E-4
24	5.045077769873823E-4	0.0	0.0	0.013495583034412475	3.783808327405367E-4
25	5.045077769873823E-4	0.0	0.0	0.014756852476880931	3.783808327405367E-4
26	5.045077769873823E-4	0.0	0.0	0.01954967635826106	3.783808327405367E-4
27	5.045077769873823E-4	0.0	0.0	0.044018303542149105	3.783808327405367E-4
28	5.045077769873823E-4	0.0	0.0	0.1716587711199568	3.783808327405367E-4
29	5.045077769873823E-4	0.0	0.0	0.3684168041450359	3.783808327405367E-4
30	5.045077769873823E-4	0.0	0.0	0.6171391381998154	3.783808327405367E-4
31	5.045077769873823E-4	0.0	0.0	1.3735224228481482	3.783808327405367E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	835	0.0	30.57485	1
ATACGGC	100	5.329639E-10	22.199999	29
TACGGCT	110	7.4578566E-11	21.863636	30
CCAGGAC	725	0.0	21.689655	3
CGGGATA	60	3.72441E-5	21.583332	16
CTTATAC	1080	0.0	21.069445	37
GCGGTAA	485	0.0	20.597939	23
CTATCCG	90	9.468931E-8	20.555557	9
ACCGTCG	45	0.0038247758	20.555557	17
CATCAGA	725	0.0	20.413794	16
ACAGGCT	725	0.0	20.15862	8
GACAGGC	745	0.0	20.114094	7
GATATTG	65	6.898679E-5	19.923077	7
TTACTCG	75	9.259826E-6	19.733334	29
TATCCGG	75	9.259826E-6	19.733334	10
TATACAC	310	0.0	19.693548	37
AACTCCG	500	0.0	19.24	5
AGGCCAT	770	0.0	19.22078	25
GGACAGG	825	0.0	19.060606	6
TATTAGA	175	0.0	19.02857	2
>>END_MODULE