FastQCFastQC Report
Fri 10 Feb 2017
ERR1631979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128959
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5270.40865701502027774No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4780.3706604424662102No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4600.3567025178545119No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4200.3256849076062935No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3940.30552346094495153No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3640.2822602532587877No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3450.2675268883908839No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3390.26287424685365113No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3130.24271280019230917No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3120.2419373599361037No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3110.24116191967989828No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3000.23263207686163823No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2570.1992881458448034No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2500.19386006405136516No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2490.1930846237951597No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2480.19230918353895426No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2280.17680037841484503No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2220.17214773687761226No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2180.1690459758527904No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2140.16594421482796856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2020.15663893175350305No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2020.15663893175350305No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1990.15431261098488666No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1980.1535371707286812No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1960.1519862902162703No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1950.1512108499600648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1940.15043540970385935No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1900.1473336486790375No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1820.14113012662939384No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1820.14113012662939384No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1810.14035468637318838No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1800.1395792461169829No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1750.1357020448359556No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1740.13492660457975014No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1730.1341511643235447No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1700.1318248435549283No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1680.1302739630425174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.1302739630425174No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1670.1294985227863119No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1650.127947642273901No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1650.127947642273901No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1630.1263967617614901No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1600.1240704409928737No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1600.1240704409928737No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1540.11941779945564095No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1540.11941779945564095No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1540.11941779945564095No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1530.11864235919943548No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1510.11709147868702455No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1490.11554059817461364No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1490.11554059817461364No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1470.11398971766220271No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1470.11398971766220271No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1440.11166339689358633No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1430.11088795663738087No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1430.11088795663738087No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1340.10390899433153174No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1340.10390899433153174No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1310.10158267356291534No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1310.10158267356291534No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1310.10158267356291534No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1300.10080723330670989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTC200.001835883137.03
GGTAGCG352.3720222E-531.7142858
CGAAATT303.5819388E-430.83333213
TAGCGAA303.5819388E-430.83333210
ATTCCTT303.5819388E-430.83333217
GTCGGGT303.5819388E-430.83333224
AAAGACT303.5819388E-430.83333236
CTTTTTA250.005478488329.62
CTATACT250.005478488329.62
CAAGGTG250.005478488329.630
AGTTCCG250.005478488329.632
CCAAGGT250.005478488329.629
CTTATAC1600.026.59375237
GAAATTC358.8265864E-426.4285714
GGAGCTA358.8265864E-426.4285720
TAAAGAC358.8265864E-426.4285735
GGGTAAG358.8265864E-426.4285727
TCGGGTA358.8265864E-426.4285725
CCGGAGC358.8265864E-426.4285718
TAAGTTC358.8265864E-426.4285730
Produced by FastQC (version 0.11.5)