##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631979.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128959
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.585100690917265	34.0	31.0	34.0	31.0	34.0
2	32.750347009514655	34.0	31.0	34.0	31.0	34.0
3	32.846679952543056	34.0	31.0	34.0	31.0	34.0
4	36.271466124892406	37.0	37.0	37.0	35.0	37.0
5	36.198753092068024	37.0	35.0	37.0	35.0	37.0
6	36.25137446785413	37.0	37.0	37.0	35.0	37.0
7	36.25315022604084	37.0	37.0	37.0	35.0	37.0
8	36.236369698896546	37.0	37.0	37.0	35.0	37.0
9	38.06710659977202	39.0	38.0	39.0	37.0	39.0
10	38.001178669189436	39.0	38.0	39.0	35.0	39.0
11	38.076318830015744	39.0	38.0	39.0	37.0	39.0
12	38.004862010406406	39.0	38.0	39.0	35.0	39.0
13	38.063756697865216	39.0	38.0	39.0	35.0	39.0
14	39.423669538380416	40.0	39.0	41.0	37.0	41.0
15	39.446583797951284	40.0	39.0	41.0	37.0	41.0
16	39.364743833311366	40.0	39.0	41.0	36.0	41.0
17	39.354725145201186	40.0	39.0	41.0	36.0	41.0
18	39.402383703347574	40.0	39.0	41.0	37.0	41.0
19	39.40218984328352	40.0	39.0	41.0	37.0	41.0
20	39.408424382943416	40.0	39.0	41.0	37.0	41.0
21	39.35137524329438	40.0	39.0	41.0	36.0	41.0
22	39.33145418311246	40.0	39.0	41.0	36.0	41.0
23	39.27592490636559	40.0	39.0	41.0	36.0	41.0
24	39.28768833505222	40.0	39.0	41.0	36.0	41.0
25	39.24162718383362	40.0	39.0	41.0	36.0	41.0
26	39.16708411200459	40.0	39.0	41.0	36.0	41.0
27	39.017703301049174	40.0	39.0	41.0	35.0	41.0
28	39.05024077419955	40.0	39.0	41.0	35.0	41.0
29	39.026822478462144	40.0	39.0	41.0	35.0	41.0
30	38.993579354678616	40.0	38.0	41.0	35.0	41.0
31	38.902573686210346	40.0	38.0	41.0	35.0	41.0
32	38.87442520491009	40.0	38.0	41.0	35.0	41.0
33	38.800393923650155	40.0	38.0	41.0	35.0	41.0
34	38.78074426755791	40.0	38.0	41.0	35.0	41.0
35	38.70358796206546	40.0	38.0	41.0	35.0	41.0
36	38.657170108329005	40.0	38.0	41.0	35.0	41.0
37	38.61087632503354	40.0	38.0	41.0	35.0	41.0
38	38.58105289277987	40.0	38.0	41.0	35.0	41.0
39	38.483758403833775	40.0	38.0	41.0	34.0	41.0
40	38.18051473724207	40.0	37.0	41.0	34.0	41.0
41	38.252460084212814	40.0	37.0	41.0	34.0	41.0
42	38.239595530362365	40.0	37.0	41.0	34.0	41.0
43	37.77796819144069	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	4.0
22	21.0
23	27.0
24	41.0
25	102.0
26	156.0
27	273.0
28	427.0
29	597.0
30	846.0
31	1192.0
32	1661.0
33	2213.0
34	3298.0
35	4892.0
36	7420.0
37	13582.0
38	31491.0
39	60713.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.931931854310285	16.41141758233237	13.20497212292279	28.45167844043456
2	21.528547833032206	19.537217255096582	31.96364736078909	26.970587551082126
3	21.135399623136035	19.47595747485635	30.029699361812668	29.35894354019495
4	14.783768484557108	16.763467458649647	36.55968175931885	31.89308229747439
5	16.309059468513247	32.90890903310355	34.26748036197551	16.514551136407697
6	30.871052039795593	35.62294992982265	16.513000255895285	16.992997774486465
7	28.725408850875084	28.272551741251096	20.19168883133399	22.810350576539832
8	26.011367954155972	30.047534487705395	21.567319845842476	22.373777712296157
9	28.494327654525858	12.350436960584371	18.04449476190107	41.1107406229887
10	19.562031343295157	22.47070774432184	28.914616273389214	29.05264463899379
11	39.327228033716146	19.30536061849115	18.709822501725355	22.657588846067355
12	22.03491032033437	24.53570514659698	26.676695693980257	26.75268883908839
13	36.23554773222497	17.602493815863955	20.131204491349962	26.03075396056111
14	23.434579982785227	21.017532704192806	23.884335331384392	31.66355198163757
15	30.481005590924248	23.7571631293667	20.06141486829147	25.70041641141758
16	23.78352809807768	24.637287820159894	23.23684271745283	28.342341364309586
17	26.570460378880114	24.139455175675987	21.46573717227956	27.824347273164342
18	26.55340069324359	20.6049984878915	23.25157608232074	29.590024736544173
19	27.832101675726395	22.547476329686177	23.539264417372962	26.081157577214466
20	30.95014694592855	19.941221628579626	22.621143154025695	26.487488271466127
21	27.276111012027076	21.95814173497003	21.91549252087873	28.850254732124164
22	28.908412751339576	23.014291363921867	20.806612954504917	27.27068293023364
23	28.17096906768818	21.764281670918663	22.453648058685317	27.61110120270784
24	27.72354003985763	20.967904527795657	23.229088314890777	28.079467117455938
25	27.875526330073903	21.858109941919523	23.47490287610791	26.791460851898663
26	28.062407431819413	23.171705735931575	22.199303654649928	26.566583177599085
27	26.520832202482957	21.79917648244791	25.15993455284238	26.52005676222676
28	25.698090090648968	23.262432245907615	22.098496421343217	28.9409812421002
29	27.34512519482936	23.841686117293094	22.244279189509843	26.5689094983677
30	26.714692266534325	21.464186291767152	24.47909800789398	27.342023433804542
31	28.287285106118997	22.7064415822083	22.814227777820857	26.192045533851843
32	25.361549019455797	21.844152017307827	23.143014446451975	29.651284516784404
33	25.228173295388455	21.628579626082708	25.467008894299738	27.676238184229096
34	25.94855729340333	21.81933792910925	24.442652315852325	27.789452461635094
35	25.074636124659776	24.186757031304523	24.997092099039232	25.741514744996476
36	25.94545553237851	22.308640730774897	25.48329313968005	26.262610597166542
37	26.8100714180476	22.30708985026249	24.804782915500276	26.078055816189643
38	25.70972169449205	21.473491574841617	25.619770624772215	27.19701610589412
39	25.042067633899144	20.39640505897223	26.744158996270134	27.81736831085849
40	24.966849929047218	21.65261827402508	27.712683876270756	25.667847920656957
41	22.644406361711862	20.46154204049349	28.66492451089106	28.22912708690359
42	20.549942229700914	21.6417621104382	30.118099551020094	27.69019610884079
43	20.206422196201892	22.245054629766052	29.357392659682535	28.19113051434952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	8.0
6	8.5
7	9.0
8	10.5
9	12.0
10	21.5
11	31.0
12	31.0
13	69.5
14	108.0
15	182.5
16	257.0
17	246.5
18	236.0
19	236.0
20	286.5
21	337.0
22	248.0
23	159.0
24	170.5
25	182.0
26	182.0
27	203.0
28	224.0
29	270.0
30	316.0
31	454.5
32	593.0
33	593.0
34	848.5
35	1104.0
36	1229.5
37	1355.0
38	1821.5
39	2288.0
40	2288.0
41	2939.0
42	3590.0
43	4495.0
44	5400.0
45	8063.5
46	10727.0
47	10727.0
48	12138.0
49	13549.0
50	15288.5
51	17028.0
52	17053.0
53	17078.0
54	17078.0
55	15195.0
56	13312.0
57	12489.0
58	11666.0
59	10737.0
60	9808.0
61	9808.0
62	8641.0
63	7474.0
64	5880.5
65	4287.0
66	3598.0
67	2909.0
68	2909.0
69	2441.5
70	1974.0
71	1653.0
72	1332.0
73	994.5
74	657.0
75	657.0
76	530.5
77	404.0
78	323.5
79	243.0
80	184.0
81	125.0
82	125.0
83	105.5
84	86.0
85	72.0
86	58.0
87	41.0
88	24.0
89	24.0
90	15.0
91	6.0
92	4.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	128959.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.81036608534495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.87481613511689	36.68840484184896
2	7.499956737674564	6.721516140788933
3	2.962604045892675	3.9826611558712455
4	1.7529894266876633	3.1420839181445266
5	1.079827642895461	2.419373599361037
6	0.7181546022461799	1.930846237951597
7	0.5987505840413935	1.8781163005296257
8	0.39801339401595515	1.4268100714180476
9	0.3391766314222922	1.3678766119464325
>10	2.408846280305259	21.25094022131065
>50	0.20938965511274163	6.309757364743833
>100	0.1557443715714607	12.472956521064834
>500	0.0017304930174606744	0.40865701502027774
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	527	0.40865701502027774	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	478	0.3706604424662102	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	460	0.3567025178545119	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	420	0.3256849076062935	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	394	0.30552346094495153	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	364	0.2822602532587877	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	345	0.2675268883908839	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	339	0.26287424685365113	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	313	0.24271280019230917	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	312	0.2419373599361037	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	311	0.24116191967989828	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	300	0.23263207686163823	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	257	0.1992881458448034	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	250	0.19386006405136516	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	249	0.1930846237951597	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	248	0.19230918353895426	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	228	0.17680037841484503	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	222	0.17214773687761226	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	218	0.1690459758527904	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	214	0.16594421482796856	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	202	0.15663893175350305	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	202	0.15663893175350305	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	199	0.15431261098488666	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	198	0.1535371707286812	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	196	0.1519862902162703	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	195	0.1512108499600648	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	194	0.15043540970385935	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	190	0.1473336486790375	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	182	0.14113012662939384	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	182	0.14113012662939384	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	181	0.14035468637318838	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	180	0.1395792461169829	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	175	0.1357020448359556	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	174	0.13492660457975014	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	173	0.1341511643235447	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	170	0.1318248435549283	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	168	0.1302739630425174	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.1302739630425174	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	167	0.1294985227863119	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	165	0.127947642273901	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	165	0.127947642273901	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	163	0.1263967617614901	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	160	0.1240704409928737	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	160	0.1240704409928737	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	154	0.11941779945564095	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	154	0.11941779945564095	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	154	0.11941779945564095	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	153	0.11864235919943548	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	151	0.11709147868702455	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	149	0.11554059817461364	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	149	0.11554059817461364	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	147	0.11398971766220271	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	147	0.11398971766220271	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	144	0.11166339689358633	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	143	0.11088795663738087	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	143	0.11088795663738087	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	134	0.10390899433153174	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	134	0.10390899433153174	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	131	0.10158267356291534	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	131	0.10158267356291534	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	131	0.10158267356291534	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	130	0.10080723330670989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	7.754402562054607E-4	0.0	0.0	0.0
11	0.0	7.754402562054607E-4	0.0	0.0	0.0
12	0.0	7.754402562054607E-4	0.0	0.0	0.0
13	0.0	7.754402562054607E-4	0.0	0.0	0.0
14	0.0	7.754402562054607E-4	0.0	0.0	0.0
15	0.0	7.754402562054607E-4	0.0	0.0	0.0
16	0.0	7.754402562054607E-4	0.0	7.754402562054607E-4	0.0
17	0.0	7.754402562054607E-4	0.0	7.754402562054607E-4	0.0
18	0.0	7.754402562054607E-4	0.0	7.754402562054607E-4	0.0
19	0.0	7.754402562054607E-4	0.0	0.0015508805124109213	0.0
20	0.0	7.754402562054607E-4	0.0	0.0015508805124109213	0.0
21	0.0	7.754402562054607E-4	0.0	0.0038772012810273032	0.0
22	0.0	7.754402562054607E-4	0.0	0.010856163586876449	0.0
23	0.0	7.754402562054607E-4	0.0	0.01163160384308191	0.0
24	0.0	7.754402562054607E-4	0.0	0.016284245380314675	0.0
25	0.0	7.754402562054607E-4	0.0	0.018610566148931057	0.0
26	0.0	7.754402562054607E-4	0.0	0.02326320768616382	0.0
27	0.0	7.754402562054607E-4	0.0	0.04497553485991672	0.0
28	0.0	7.754402562054607E-4	0.0	0.15508805124109212	0.0
29	0.0	7.754402562054607E-4	0.0	0.31793050504423886	0.0
30	0.0	7.754402562054607E-4	0.0	0.5490117013934661	0.0
31	0.0	7.754402562054607E-4	0.0	1.1437743779030545	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTC	20	0.0018358831	37.0	3
GGTAGCG	35	2.3720222E-5	31.714285	8
CGAAATT	30	3.5819388E-4	30.833332	13
TAGCGAA	30	3.5819388E-4	30.833332	10
ATTCCTT	30	3.5819388E-4	30.833332	17
GTCGGGT	30	3.5819388E-4	30.833332	24
AAAGACT	30	3.5819388E-4	30.833332	36
CTTTTTA	25	0.0054784883	29.6	2
CTATACT	25	0.0054784883	29.6	2
CAAGGTG	25	0.0054784883	29.6	30
AGTTCCG	25	0.0054784883	29.6	32
CCAAGGT	25	0.0054784883	29.6	29
CTTATAC	160	0.0	26.593752	37
GAAATTC	35	8.8265864E-4	26.42857	14
GGAGCTA	35	8.8265864E-4	26.42857	20
TAAAGAC	35	8.8265864E-4	26.42857	35
GGGTAAG	35	8.8265864E-4	26.42857	27
TCGGGTA	35	8.8265864E-4	26.42857	25
CCGGAGC	35	8.8265864E-4	26.42857	18
TAAGTTC	35	8.8265864E-4	26.42857	30
>>END_MODULE