FastQCFastQC Report
Fri 10 Feb 2017
ERR1631978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268375
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8020.2988355845365626No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7480.2787144853283652No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7160.26679087098276666No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6610.2462971588262692No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5690.2120167675826735No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5580.20791802515137403No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5450.20307405682347462No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5350.1993479273404751No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5320.19823008849557522No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4940.184070796460177No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4800.17885421518397765No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4770.17773637633907777No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4360.16245924545877968No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3630.13525850023288308No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3370.1255705635770843No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3280.12221704704238473No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3260.12147182114578482No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3220.11998136935258501No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3200.11923614345598509No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3190.11886353050768514No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3160.11774569166278527No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3080.11476478807638565No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3060.11401956217978575No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2950.10992081974848626No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2830.10544946436888683No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2810.10470423847228692No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2800.10433162552398696No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2790.10395901257568702No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2740.10209594783418723No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2730.10172333488588728No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2690.10023288309268746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC507.2886905E-933.30000329
TGATACA250.005488930329.64
CGCATAA250.005488930329.610
TATTAGA250.005488930329.617
GTAAATT250.005488930329.620
GGTATCA3450.027.8840581
TACGGCT604.2968168E-827.75000230
CTTATCC358.851615E-426.4285723
CCGTGTC509.057223E-625.9000029
GTCGACA451.319523E-424.66666622
CAGTCGG701.9115214E-723.78571510
CTCTATG1400.023.7857151
ATTGATA551.8956218E-523.54545431
AAAAACA400.001927353123.12522
TATCCTT400.001927353123.1255
TATTGAT652.6701418E-622.7692330
CTTATTG652.6701418E-622.7692328
CTTATAC3050.022.44262337
TACCAAA502.6946294E-422.218
TTATCCT502.6946294E-422.24
Produced by FastQC (version 0.11.5)