FastQCFastQC Report
Fri 10 Feb 2017
ERR1631977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482890
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11510.23835656153575346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.23711404253556712No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9640.1996313860299447No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9600.19880304002982047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9340.19341879102901283No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9080.1880345420282052No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8680.17975108202696266No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7620.15779991302367No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7530.15593613452339042No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7490.15510778852326615No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7390.15303692352295553No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7270.15055188552258278No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6740.13957630102093646No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6560.13584874402037733No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6180.12797945701919694No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5980.12383772701857565No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5610.11617552651742633No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5450.11286214251692932No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5130.10623537451593532No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5060.10478576901571787No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4970.1029219905154383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.030.8685681
AACCTCT303.5964142E-430.83333436
GACTATG250.00549322629.67
AATACAC358.8619185E-426.428572
TCGTGTT551.8996978E-523.54545415
ACTGTGC1155.456968E-1222.521748
ACCATTG753.731475E-722.28
CACGAAA1005.329639E-1022.218
AAACACG1005.329639E-1022.215
CGTGTTT502.6989583E-422.216
ATACACA1250.022.237
ACACGAA1059.786163E-1021.14285917
ATTGAAG705.091819E-621.1428573
CCCTATG450.00382264420.5555552
TATAGGG450.00382264420.5555552
ACATAGA450.00382264420.5555551
CAAACAC1101.7480488E-920.18181814
TTCGTGT555.1371596E-420.18181814
GGACGTA1101.7480488E-920.18181827
CTACCCA555.1371596E-420.18181819
Produced by FastQC (version 0.11.5)