##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631977.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482890
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62459773447369	34.0	31.0	34.0	31.0	34.0
2	32.789415808983414	34.0	31.0	34.0	31.0	34.0
3	32.87540226552631	34.0	31.0	34.0	31.0	34.0
4	36.29970179543996	37.0	37.0	37.0	35.0	37.0
5	36.22343805007352	37.0	35.0	37.0	35.0	37.0
6	36.27500051771625	37.0	37.0	37.0	35.0	37.0
7	36.267673797345154	37.0	37.0	37.0	35.0	37.0
8	36.25479508790822	37.0	37.0	37.0	35.0	37.0
9	38.080931475077136	39.0	38.0	39.0	37.0	39.0
10	38.0220070823583	39.0	38.0	39.0	35.0	39.0
11	38.089384746008406	39.0	38.0	39.0	37.0	39.0
12	38.02720288264408	39.0	38.0	39.0	35.0	39.0
13	38.07991675122699	39.0	38.0	39.0	37.0	39.0
14	39.47313259748597	40.0	39.0	41.0	37.0	41.0
15	39.47904698792686	41.0	39.0	41.0	37.0	41.0
16	39.427296071569096	40.0	39.0	41.0	37.0	41.0
17	39.39958582699994	40.0	39.0	41.0	37.0	41.0
18	39.43081447120462	41.0	39.0	41.0	37.0	41.0
19	39.45650148066848	41.0	39.0	41.0	37.0	41.0
20	39.443419826461515	41.0	39.0	41.0	37.0	41.0
21	39.40995257719149	40.0	39.0	41.0	37.0	41.0
22	39.38728281803309	40.0	39.0	41.0	36.0	41.0
23	39.321302988258196	40.0	39.0	41.0	36.0	41.0
24	39.338561577170786	41.0	39.0	41.0	36.0	41.0
25	39.275294580546294	40.0	39.0	41.0	36.0	41.0
26	39.21979539853797	40.0	39.0	41.0	36.0	41.0
27	39.11719232123258	40.0	39.0	41.0	36.0	41.0
28	39.11445463770217	40.0	39.0	41.0	35.0	41.0
29	39.09154879993373	40.0	39.0	41.0	35.0	41.0
30	39.03818260887573	40.0	39.0	41.0	35.0	41.0
31	38.94812276087722	40.0	38.0	41.0	35.0	41.0
32	38.892691917413906	40.0	38.0	41.0	35.0	41.0
33	38.825684938598855	40.0	38.0	41.0	35.0	41.0
34	38.793574105904035	40.0	38.0	41.0	35.0	41.0
35	38.72586096212388	40.0	38.0	41.0	35.0	41.0
36	38.67190043281079	40.0	38.0	41.0	35.0	41.0
37	38.62590652115389	40.0	38.0	41.0	35.0	41.0
38	38.57840501977676	40.0	38.0	41.0	35.0	41.0
39	38.50288264408043	40.0	38.0	41.0	35.0	41.0
40	38.18056700283709	40.0	38.0	41.0	34.0	41.0
41	38.235956429000396	40.0	37.0	41.0	34.0	41.0
42	38.218418273312764	40.0	37.0	41.0	34.0	41.0
43	37.744948124831744	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	1.0
16	1.0
17	2.0
18	4.0
19	17.0
20	16.0
21	34.0
22	78.0
23	132.0
24	206.0
25	364.0
26	663.0
27	962.0
28	1531.0
29	2294.0
30	3156.0
31	4490.0
32	6009.0
33	8119.0
34	11663.0
35	17731.0
36	27534.0
37	50607.0
38	112476.0
39	234798.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82590237942389	16.836960798525542	13.051419577957713	27.285717244092854
2	20.228830582534325	20.162355816024352	33.332021785499805	26.276791815941518
3	21.110811986166624	20.70347284060552	29.724367868458657	28.461347304769202
4	15.24860734328729	16.55946488848392	34.52256207417839	33.669365694050406
5	16.60833730249125	33.17318643997598	33.62049328004307	16.597982977489696
6	32.555861583383376	34.369939323655494	16.01979746940297	17.05440162355816
7	29.355960984903394	28.672161361800825	20.471743047070763	21.50013460622502
8	26.904678084035698	31.159684400173955	20.54940048458241	21.386237031207937
9	28.16624904222494	13.400981590010147	18.293193066743978	40.13957630102094
10	19.173103605375967	23.992420634098863	30.105821201515877	26.728654559009296
11	37.63300130464495	20.518130423077718	19.620617531943093	22.22825074033424
12	22.284578268342685	24.618857296692827	27.248855847087327	25.847708587877154
13	34.06987098511048	18.35552610325333	21.72151007475823	25.853092836877966
14	23.73107747105966	20.896891631634535	24.21172523763176	31.160305659674048
15	28.940545465841083	24.949160264242373	20.8573381101286	25.252956159787942
16	24.608295885191247	24.96841930874526	23.470355567520553	26.95292923854294
17	25.951044751392654	24.58820849468823	22.818240178922736	26.64250657499638
18	25.721178736358176	22.222038145333308	24.493362877674006	27.56342024063451
19	27.234773965085218	23.20632027998095	24.548033713682205	25.01087204125163
20	28.829754188324465	21.621694381743254	24.0833316076125	25.465219822319785
21	26.469796433970473	23.244424195986664	23.104019548965603	27.18175982107726
22	27.14344881857152	23.855122284578268	22.64780798939717	26.353620907453045
23	26.82432852202365	23.13487543747023	23.727971173559194	26.312824866946922
24	26.837996231025702	22.7213236969082	23.935264760090288	26.50541531197581
25	26.674604982501194	23.104226635465633	24.25293545113794	25.968232930895237
26	26.60026092899004	23.779121539066868	23.64927830354739	25.971339228395703
27	26.31178943444677	22.691089067903665	24.87419495123113	26.12292654641844
28	25.578081964836713	23.70539874505581	23.390213092008533	27.326306198098948
29	26.225434363933815	24.20737642113111	23.47573981652136	26.09144939841372
30	26.036985648905546	23.105469154465823	24.927623268239145	25.929921928389486
31	26.610408167491563	23.2593344239889	24.010230073101535	26.120027335418005
32	24.473689660171054	23.357907598003685	24.341361386651204	27.827041355174053
33	24.857628031228643	22.83874174242581	25.886640849882998	26.416989376462546
34	25.29271676779391	22.780550435917082	25.19724989127959	26.729482905009423
35	24.81766033672265	24.06614342810992	25.454244237818134	25.66195199734929
36	25.091221603263687	22.98473772494771	25.88705502288306	26.036985648905546
37	25.51699144732755	22.510095466876514	25.52341112882851	26.449501956967424
38	24.92907287373936	22.030690219304603	26.329598873449438	26.710638033506594
39	24.449253453167387	21.005612044150844	27.579365901136903	26.965768601544866
40	24.158504007123778	22.087639006813145	27.906355484685953	25.847501501377124
41	22.70413551740562	21.460166911719025	28.966017105344903	26.869680465530454
42	21.430553542214582	21.913686346787053	29.540060883431007	27.11569922756735
43	20.732257864109837	22.505539563875832	29.211828780881778	27.550373791132554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	9.0
2	11.0
3	33.5
4	56.0
5	56.0
6	75.0
7	94.0
8	110.0
9	126.0
10	196.0
11	266.0
12	266.0
13	535.0
14	804.0
15	1513.5
16	2223.0
17	2189.0
18	2155.0
19	2155.0
20	2449.5
21	2744.0
22	2329.0
23	1914.0
24	2036.5
25	2159.0
26	2159.0
27	2361.0
28	2563.0
29	3222.0
30	3881.0
31	4582.0
32	5283.0
33	5283.0
34	6442.5
35	7602.0
36	8501.0
37	9400.0
38	11507.5
39	13615.0
40	13615.0
41	15980.5
42	18346.0
43	20872.5
44	23399.0
45	30524.0
46	37649.0
47	37649.0
48	41772.5
49	45896.0
50	50776.0
51	55656.0
52	56196.0
53	56736.0
54	56736.0
55	50739.5
56	44743.0
57	41365.5
58	37988.0
59	34438.5
60	30889.0
61	30889.0
62	28011.0
63	25133.0
64	20657.0
65	16181.0
66	13997.0
67	11813.0
68	11813.0
69	9967.5
70	8122.0
71	6923.5
72	5725.0
73	4706.0
74	3687.0
75	3687.0
76	2994.5
77	2302.0
78	1893.5
79	1485.0
80	1195.5
81	906.0
82	906.0
83	753.5
84	601.0
85	488.5
86	376.0
87	286.0
88	196.0
89	196.0
90	157.5
91	119.0
92	76.5
93	34.0
94	19.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	482890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.2725707489346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.74095302184713	41.38936220828194
2	6.473264805606384	6.24962266610445
3	2.2273627505884432	3.2256157788396655
4	1.153796348240093	2.227868634011291
5	0.7672416299518799	1.851836293169001
6	0.5008443700661183	1.4506227172933364
7	0.3872218128644975	1.3084534649922517
8	0.2992009062793559	1.1554557533212448
9	0.23752054475930764	1.0319154571097249
>10	1.8599269643117127	18.02324114737385
>50	0.21901559597391618	7.378859599886327
>100	0.1250285882523981	11.516473693929015
>500	0.007760395132907468	2.7128313322315436
>1k	8.622661258786074E-4	0.47784125345635786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1151	0.23835656153575346	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1145	0.23711404253556712	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	964	0.1996313860299447	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	960	0.19880304002982047	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.19341879102901283	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	908	0.1880345420282052	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	868	0.17975108202696266	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	762	0.15779991302367	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	753	0.15593613452339042	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	749	0.15510778852326615	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	739	0.15303692352295553	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	727	0.15055188552258278	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	674	0.13957630102093646	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	656	0.13584874402037733	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	618	0.12797945701919694	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	598	0.12383772701857565	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	561	0.11617552651742633	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	545	0.11286214251692932	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	513	0.10623537451593532	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	506	0.10478576901571787	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	497	0.1029219905154383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.0708650003106299E-4	0.0
4	0.0	0.0	0.0	2.0708650003106299E-4	0.0
5	0.0	0.0	0.0	2.0708650003106299E-4	0.0
6	0.0	0.0	0.0	2.0708650003106299E-4	0.0
7	0.0	0.0	0.0	2.0708650003106299E-4	0.0
8	4.1417300006212597E-4	0.0	0.0	2.0708650003106299E-4	0.0
9	4.1417300006212597E-4	0.0	0.0	2.0708650003106299E-4	0.0
10	4.1417300006212597E-4	0.0	0.0	2.0708650003106299E-4	0.0
11	4.1417300006212597E-4	0.0	0.0	2.0708650003106299E-4	0.0
12	4.1417300006212597E-4	0.0	0.0	4.1417300006212597E-4	0.0
13	4.1417300006212597E-4	0.0	0.0	4.1417300006212597E-4	0.0
14	4.1417300006212597E-4	0.0	0.0	6.21259500093189E-4	0.0
15	4.1417300006212597E-4	0.0	0.0	6.21259500093189E-4	0.0
16	4.1417300006212597E-4	0.0	0.0	0.001035432500155315	0.0
17	4.1417300006212597E-4	0.0	0.0	0.0018637785002795669	0.0
18	4.1417300006212597E-4	0.0	0.0	0.002277951500341693	0.0
19	4.1417300006212597E-4	0.0	0.0	0.002692124500403819	0.0
20	4.1417300006212597E-4	0.0	0.0	0.00414173000062126	0.0
21	6.21259500093189E-4	0.0	0.0	0.0062125950009318896	0.0
22	8.283460001242519E-4	0.0	0.0	0.009318892501397833	0.0
23	8.283460001242519E-4	0.0	0.0	0.011182671001677401	0.0
24	8.283460001242519E-4	0.0	0.0	0.013667709002050157	0.0
25	8.283460001242519E-4	0.0	0.0	0.015324401002298661	0.0
26	8.283460001242519E-4	0.0	0.0	0.01946613100291992	0.0
27	8.283460001242519E-4	0.0	0.0	0.047836981507175545	0.0
28	8.283460001242519E-4	0.0	0.0	0.16960384352544058	0.0
29	8.283460001242519E-4	0.0	0.0	0.3545320880531798	0.0
30	8.283460001242519E-4	0.0	0.0	0.5736296050860444	0.0
31	8.283460001242519E-4	0.0	0.0	1.1998591811799788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	875	0.0	30.868568	1
AACCTCT	30	3.5964142E-4	30.833334	36
GACTATG	25	0.005493226	29.6	7
AATACAC	35	8.8619185E-4	26.42857	2
TCGTGTT	55	1.8996978E-5	23.545454	15
ACTGTGC	115	5.456968E-12	22.52174	8
ACCATTG	75	3.731475E-7	22.2	8
CACGAAA	100	5.329639E-10	22.2	18
AAACACG	100	5.329639E-10	22.2	15
CGTGTTT	50	2.6989583E-4	22.2	16
ATACACA	125	0.0	22.2	37
ACACGAA	105	9.786163E-10	21.142859	17
ATTGAAG	70	5.091819E-6	21.142857	3
CCCTATG	45	0.003822644	20.555555	2
TATAGGG	45	0.003822644	20.555555	2
ACATAGA	45	0.003822644	20.555555	1
CAAACAC	110	1.7480488E-9	20.181818	14
TTCGTGT	55	5.1371596E-4	20.181818	14
GGACGTA	110	1.7480488E-9	20.181818	27
CTACCCA	55	5.1371596E-4	20.181818	19
>>END_MODULE