FastQCFastQC Report
Fri 10 Feb 2017
ERR1631976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4689
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA340.7251013009170398No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA250.5331627212625294No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA250.5331627212625294No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA240.5118362124120281No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC240.5118362124120281No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA220.46918319471102576No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT180.3838771593090211No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA180.3838771593090211No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC160.3412241416080187No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA160.3412241416080187No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG150.3198976327575176No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC140.2985711239070164No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT140.2985711239070164TruSeq Adapter, Index 5 (95% over 21bp)
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA140.2985711239070164No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA130.27724461505651526No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA130.27724461505651526No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT120.2559181062060141No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA120.2559181062060141No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC120.2559181062060141No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT110.23459159735551288No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC110.23459159735551288No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC110.23459159735551288No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG110.23459159735551288No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC100.21326508850501172No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT100.21326508850501172No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT100.21326508850501172No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG100.21326508850501172No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC100.21326508850501172No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG100.21326508850501172No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA90.19193857965451055No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA90.19193857965451055No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA90.19193857965451055No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC90.19193857965451055No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG90.19193857965451055No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT90.19193857965451055No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA90.19193857965451055No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC90.19193857965451055No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC90.19193857965451055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19193857965451055No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA90.19193857965451055No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT90.19193857965451055No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT90.19193857965451055No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC90.19193857965451055No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA90.19193857965451055No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT90.19193857965451055No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT90.19193857965451055No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA80.17061207080400936No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG80.17061207080400936No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC80.17061207080400936No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC80.17061207080400936No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC80.17061207080400936No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG80.17061207080400936No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA80.17061207080400936No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG70.1492855619535082No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA70.1492855619535082No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA70.1492855619535082No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT70.1492855619535082No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT70.1492855619535082No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA70.1492855619535082No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA70.1492855619535082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.1492855619535082No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC70.1492855619535082No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA70.1492855619535082No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA70.1492855619535082No Hit
ATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGAC60.12795905310300704No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA60.12795905310300704No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT60.12795905310300704No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC60.12795905310300704No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACC60.12795905310300704No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA60.12795905310300704No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC60.12795905310300704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60.12795905310300704No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC60.12795905310300704No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC60.12795905310300704No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA60.12795905310300704No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG60.12795905310300704No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT60.12795905310300704No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG60.12795905310300704No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT60.12795905310300704No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG60.12795905310300704No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC60.12795905310300704No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA50.10663254425250586No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT50.10663254425250586No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC50.10663254425250586No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGCT50.10663254425250586No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC50.10663254425250586No Hit
TGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTC50.10663254425250586No Hit
GCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG50.10663254425250586No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT50.10663254425250586No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC50.10663254425250586No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG50.10663254425250586No Hit
GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC50.10663254425250586No Hit
CCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCG50.10663254425250586No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC50.10663254425250586No Hit
GTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGAC50.10663254425250586No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC50.10663254425250586No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT50.10663254425250586No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG50.10663254425250586No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA50.10663254425250586No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA50.10663254425250586No Hit
TAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACAC50.10663254425250586No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT50.10663254425250586No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATG50.10663254425250586No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT50.10663254425250586No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA50.10663254425250586No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers