FastQCFastQC Report
Fri 10 Feb 2017
ERR1631975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616928
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17790.2883642823797915No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16370.26534700969967323No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16310.26437444888220346No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15850.2569181492816017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13900.22530992271383368No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13670.22158177291353287No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13420.2175294361740754No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13220.21428756678250946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.2120182582084133No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12380.2006717153379325No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11760.190621920224078No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11620.18835261164998185No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11160.18089631204938017No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10960.17765444265781422No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10830.17554722755329633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10560.1711707038746823No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9400.15236786140359976No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8340.13518595362830022No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC8300.13453757974998704No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7720.12513615851444576No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7680.12448778463613258No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7450.12075963483583173No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7380.11962498054878365No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7350.11913870014004875No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7230.11719357850510918No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7140.1157347372789045No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7130.1155726438093262No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7100.11508636340059131No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6930.11233077441776027No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6900.11184449400902535No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6810.11038565278282067No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6740.1092509984957726No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6690.10844053114788112No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6690.10844053114788112No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6670.10811634420872453No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6640.10763006379998963No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6570.10649540951294155No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6290.10195679236474921No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6290.10195679236474921No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6270.1016326054255926No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6180.10017376419938795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCC303.5975617E-430.8333343
GGTATCA10550.029.6350731
TTCTAGC250.005494393429.62
GTCTATA250.005494393429.62
TAACCCT803.274181E-1127.75000235
TATCTAG358.86472E-426.428571
GGTCTAT451.3222416E-424.6666661
TATACCC451.3222416E-424.6666665
TATTAGC701.9199251E-723.7857132
ATCTAGA400.001930178123.1250022
TCTATAC400.001930178123.1250023
CTAGAGT806.949358E-720.81254
ATACGGC806.949358E-720.812529
GTATTAG1251.8189894E-1120.721
CGACTGA450.003823829320.5555556
GACTGAT450.003823829320.5555557
TACCGCT450.003823829320.55555535
TAGCGAA555.1393826E-420.18181810
GTATCAA15750.019.4984132
TAGAGTC801.6146923E-518.55
Produced by FastQC (version 0.11.5)