##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631975.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	616928
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58374559105763	34.0	31.0	34.0	31.0	34.0
2	32.746827830800356	34.0	31.0	34.0	31.0	34.0
3	32.840138557497795	34.0	31.0	34.0	31.0	34.0
4	36.27547136780954	37.0	37.0	37.0	35.0	37.0
5	36.193982117848435	37.0	35.0	37.0	35.0	37.0
6	36.245098293479955	37.0	37.0	37.0	35.0	37.0
7	36.23635172986151	37.0	37.0	37.0	35.0	37.0
8	36.22237927278386	37.0	37.0	37.0	35.0	37.0
9	38.047841887546035	39.0	38.0	39.0	35.0	39.0
10	37.97994417500908	39.0	38.0	39.0	35.0	39.0
11	38.0541586700555	39.0	38.0	39.0	35.0	39.0
12	37.98639711603299	39.0	38.0	39.0	35.0	39.0
13	38.04009381970019	39.0	38.0	39.0	35.0	39.0
14	39.41536775766378	40.0	39.0	41.0	37.0	41.0
15	39.42586979355776	40.0	39.0	41.0	37.0	41.0
16	39.358288163286474	40.0	39.0	41.0	36.0	41.0
17	39.34249215467607	40.0	39.0	41.0	36.0	41.0
18	39.37521882618393	40.0	39.0	41.0	36.0	41.0
19	39.389857487421544	40.0	39.0	41.0	37.0	41.0
20	39.38678581617304	40.0	39.0	41.0	37.0	41.0
21	39.34653152393796	40.0	39.0	41.0	36.0	41.0
22	39.31912800197106	40.0	39.0	41.0	36.0	41.0
23	39.259860145754445	40.0	39.0	41.0	36.0	41.0
24	39.27266715078583	40.0	39.0	41.0	36.0	41.0
25	39.21325827325069	40.0	39.0	41.0	36.0	41.0
26	39.15201773950931	40.0	39.0	41.0	36.0	41.0
27	39.026943176513306	40.0	39.0	41.0	35.0	41.0
28	39.02876672804606	40.0	39.0	41.0	35.0	41.0
29	38.99708555941698	40.0	39.0	41.0	35.0	41.0
30	38.9518744488822	40.0	38.0	41.0	35.0	41.0
31	38.84310810986047	40.0	38.0	41.0	35.0	41.0
32	38.791682335702056	40.0	38.0	41.0	35.0	41.0
33	38.72183301779138	40.0	38.0	41.0	35.0	41.0
34	38.68648691581514	40.0	38.0	41.0	35.0	41.0
35	38.610776298044506	40.0	38.0	41.0	35.0	41.0
36	38.55404682556149	40.0	38.0	41.0	35.0	41.0
37	38.4899275118004	40.0	38.0	41.0	35.0	41.0
38	38.44959865656932	40.0	38.0	41.0	34.0	41.0
39	38.35624416463509	40.0	38.0	41.0	34.0	41.0
40	38.022250570569014	40.0	37.0	41.0	34.0	41.0
41	38.07152050158203	40.0	37.0	41.0	34.0	41.0
42	38.05301266922558	40.0	37.0	41.0	34.0	41.0
43	37.5638826572955	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	0.0
16	0.0
17	0.0
18	3.0
19	9.0
20	17.0
21	37.0
22	88.0
23	150.0
24	311.0
25	522.0
26	889.0
27	1424.0
28	2206.0
29	3052.0
30	4328.0
31	6011.0
32	8153.0
33	10968.0
34	15929.0
35	23893.0
36	36990.0
37	67394.0
38	150200.0
39	284351.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.25204886145547	16.308386067742102	12.664362778152395	27.775202292650036
2	20.696742569635354	19.61995565122672	32.37282146376887	27.310480315369055
3	21.45517791379221	19.70473053581617	29.356910368795063	29.483181181596557
4	15.129642356968724	16.364308314746616	34.66563359095389	33.84041573733077
5	16.859341770838736	32.63670963224234	33.481540795684424	17.022407801234504
6	32.87871518232273	34.138829814824426	16.104472483012604	16.87798251984024
7	29.879337621245917	28.05448934073344	20.19846724415167	21.86770579386898
8	27.372075833808807	30.12507132112661	20.369962134965505	22.13289071009907
9	28.507378494735203	12.87524638207376	18.00712562892266	40.61024949426837
10	19.57748716219721	23.240313294258	29.423368691322167	27.758830852222626
11	38.93971419679444	19.563709217283055	19.108388661237615	22.388187924684892
12	22.433574096166815	24.024521500077807	26.992290834586857	26.549613569168525
13	35.30979303905804	17.61307640437782	20.762228331344986	26.31490222521915
14	23.72886301156699	20.52767908086519	23.763226827117588	31.980231080450235
15	29.723727890450753	24.176727268011827	20.135737071424867	25.963807770112556
16	24.60384356035064	24.460228746304267	23.22491441464806	27.711013278697028
17	26.352345816691734	24.063748119715754	22.163364282379792	27.42054178121272
18	26.667131334612794	21.31431868872867	23.67083354945796	28.34771642720058
19	27.59300923284403	22.79990533741377	23.956604336324496	25.65048109341771
20	29.73426396597334	20.958847709943463	23.110314331656205	26.19657399242699
21	27.348734374189533	22.261430831474662	22.530343897505055	27.859490896830746
22	28.03746952642772	23.047908345868564	22.040497432439444	26.87412469526428
23	27.81637403392292	22.10257923128793	22.870415996680325	27.210630738108822
24	27.431726230613624	21.729277970849108	23.540996680325744	27.297999118211525
25	27.37774910524405	22.203887649774366	23.75350121894289	26.664862026038694
26	27.505478759271746	23.07465376834898	22.977397686602004	26.442469785777273
27	26.526596296488407	22.114087867627987	24.71439130660304	26.644924529280566
28	26.186362103843557	23.14046371699777	22.53131645832253	28.141857720836143
29	26.853052544219096	23.732104880958556	22.68789874993516	26.726943824887183
30	26.726943824887183	22.124137662741845	24.198285699465742	26.950632812905233
31	27.465117485346752	22.664557290315887	23.32346724415167	26.546857980185695
32	25.620493801545724	22.518673167695418	23.39592302505317	28.46491000570569
33	25.448836817262304	22.27715389802376	25.0627301727268	27.211279111987135
34	26.067223403703508	22.51397245707765	24.113997095285026	27.304807043933817
35	25.35385004408942	23.961791327351005	24.798842004253334	25.885516624306238
36	26.11017817314176	22.611552725763783	24.973578764458736	26.304690336635716
37	26.42885393433269	22.67963198298667	24.690239379635873	26.201274703044763
38	25.607364230509884	22.07405078064215	25.194025883085224	27.124559105762746
39	25.247192541106905	21.19890813838892	26.19462887079205	27.35927044971212
40	25.067755070283727	22.093339903521965	26.72548498366098	26.113420042533324
41	23.404027698532083	21.7667215623217	27.480030084547952	27.349220654598266
42	21.960423258467763	22.157691010944554	28.420820581980394	27.461065148607293
43	21.50445432854401	22.954218320452306	27.829179418019606	27.712147932984077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	12.5
2	16.0
3	30.0
4	44.0
5	44.0
6	68.5
7	93.0
8	97.5
9	102.0
10	169.5
11	237.0
12	237.0
13	501.5
14	766.0
15	1501.5
16	2237.0
17	2210.5
18	2184.0
19	2184.0
20	2550.5
21	2917.0
22	2319.5
23	1722.0
24	1766.5
25	1811.0
26	1811.0
27	2035.0
28	2259.0
29	2714.0
30	3169.0
31	3872.5
32	4576.0
33	4576.0
34	5949.5
35	7323.0
36	8233.5
37	9144.0
38	11349.0
39	13554.0
40	13554.0
41	16877.5
42	20201.0
43	23713.0
44	27225.0
45	38186.0
46	49147.0
47	49147.0
48	54013.5
49	58880.0
50	65849.5
51	72819.0
52	71465.5
53	70112.0
54	70112.0
55	63185.0
56	56258.0
57	53110.0
58	49962.0
59	46979.5
60	43997.0
61	43997.0
62	40537.5
63	37078.0
64	30609.5
65	24141.0
66	21313.5
67	18486.0
68	18486.0
69	15791.0
70	13096.0
71	10970.5
72	8845.0
73	7200.0
74	5555.0
75	5555.0
76	4577.5
77	3600.0
78	2936.5
79	2273.0
80	1879.0
81	1485.0
82	1485.0
83	1158.5
84	832.0
85	633.0
86	434.0
87	323.0
88	212.0
89	212.0
90	153.0
91	94.0
92	58.5
93	23.0
94	15.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	616928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.21795107623678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.60559939506163	42.625501539543045
2	6.7915453376410975	6.68531892320127
3	2.0909089780410066	3.0873076735825946
4	1.0621493723660804	2.0910726337907737
5	0.647917716455871	1.5944591234976058
6	0.4176489896285639	1.2333496523147025
7	0.3066749184296151	1.056573779210439
8	0.21642316173643283	0.8521523668886585
9	0.1749714282830854	0.7750561677278552
>10	1.3580589578533804	13.410408714255206
>50	0.17845291698996482	6.1100301928059775
>100	0.13077718333569976	12.667688156805113
>500	0.013574340548768837	4.34216913408181
>1k	0.005297303628787838	3.4689119422949513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1779	0.2883642823797915	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1637	0.26534700969967323	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1631	0.26437444888220346	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1585	0.2569181492816017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1390	0.22530992271383368	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1367	0.22158177291353287	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1342	0.2175294361740754	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1322	0.21428756678250946	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.2120182582084133	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1238	0.2006717153379325	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1176	0.190621920224078	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1162	0.18835261164998185	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1116	0.18089631204938017	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1096	0.17765444265781422	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1083	0.17554722755329633	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.1711707038746823	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	940	0.15236786140359976	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	834	0.13518595362830022	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	830	0.13453757974998704	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	772	0.12513615851444576	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	768	0.12448778463613258	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	745	0.12075963483583173	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	738	0.11962498054878365	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	735	0.11913870014004875	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	723	0.11719357850510918	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	714	0.1157347372789045	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	713	0.1155726438093262	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	710	0.11508636340059131	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	693	0.11233077441776027	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	690	0.11184449400902535	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	681	0.11038565278282067	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	674	0.1092509984957726	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	669	0.10844053114788112	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	669	0.10844053114788112	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	667	0.10811634420872453	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	664	0.10763006379998963	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	657	0.10649540951294155	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	629	0.10195679236474921	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	629	0.10195679236474921	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	627	0.1016326054255926	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	618	0.10017376419938795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6209346957829764E-4
2	0.0	0.0	0.0	0.0	1.6209346957829764E-4
3	0.0	0.0	0.0	0.0	1.6209346957829764E-4
4	0.0	0.0	0.0	0.0	1.6209346957829764E-4
5	0.0	0.0	0.0	0.0	1.6209346957829764E-4
6	0.0	0.0	0.0	0.0	1.6209346957829764E-4
7	0.0	0.0	0.0	0.0	1.6209346957829764E-4
8	1.6209346957829764E-4	0.0	0.0	0.0	1.6209346957829764E-4
9	1.6209346957829764E-4	0.0	0.0	0.0	1.6209346957829764E-4
10	3.241869391565953E-4	0.0	0.0	0.0	1.6209346957829764E-4
11	3.241869391565953E-4	0.0	0.0	0.0	1.6209346957829764E-4
12	3.241869391565953E-4	0.0	0.0	0.0	1.6209346957829764E-4
13	4.862804087348929E-4	0.0	0.0	0.0	1.6209346957829764E-4
14	4.862804087348929E-4	0.0	0.0	0.0	1.6209346957829764E-4
15	6.483738783131906E-4	0.0	0.0	0.0	1.6209346957829764E-4
16	6.483738783131906E-4	0.0	0.0	4.862804087348929E-4	1.6209346957829764E-4
17	6.483738783131906E-4	0.0	0.0	4.862804087348929E-4	3.241869391565953E-4
18	8.104673478914881E-4	0.0	0.0	6.483738783131906E-4	3.241869391565953E-4
19	8.104673478914881E-4	0.0	0.0	8.104673478914881E-4	3.241869391565953E-4
20	8.104673478914881E-4	0.0	0.0	0.0014588412262046787	3.241869391565953E-4
21	9.725608174697858E-4	0.0	0.0	0.002107215104517869	3.241869391565953E-4
22	0.0011346542870480834	0.0	0.0	0.0037281498003008456	3.241869391565953E-4
23	0.0011346542870480834	0.0	0.0	0.005673271435240417	3.241869391565953E-4
24	0.0011346542870480834	0.0	0.0	0.007618393070179989	3.241869391565953E-4
25	0.0011346542870480834	0.0	0.0	0.00826676694849318	3.241869391565953E-4
26	0.0011346542870480834	0.0	0.0	0.01102235593132424	3.241869391565953E-4
27	0.0011346542870480834	0.0	0.0	0.029825198402406765	3.241869391565953E-4
28	0.0011346542870480834	0.0	0.0	0.1021188858343275	4.862804087348929E-4
29	0.0011346542870480834	0.0	0.0	0.2244994553659422	4.862804087348929E-4
30	0.0011346542870480834	0.0	0.0	0.3901589812749624	4.862804087348929E-4
31	0.0011346542870480834	0.0	0.0	0.836564396493594	4.862804087348929E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCC	30	3.5975617E-4	30.833334	3
GGTATCA	1055	0.0	29.635073	1
TTCTAGC	25	0.0054943934	29.6	2
GTCTATA	25	0.0054943934	29.6	2
TAACCCT	80	3.274181E-11	27.750002	35
TATCTAG	35	8.86472E-4	26.42857	1
GGTCTAT	45	1.3222416E-4	24.666666	1
TATACCC	45	1.3222416E-4	24.666666	5
TATTAGC	70	1.9199251E-7	23.785713	2
ATCTAGA	40	0.0019301781	23.125002	2
TCTATAC	40	0.0019301781	23.125002	3
CTAGAGT	80	6.949358E-7	20.8125	4
ATACGGC	80	6.949358E-7	20.8125	29
GTATTAG	125	1.8189894E-11	20.72	1
CGACTGA	45	0.0038238293	20.555555	6
GACTGAT	45	0.0038238293	20.555555	7
TACCGCT	45	0.0038238293	20.555555	35
TAGCGAA	55	5.1393826E-4	20.181818	10
GTATCAA	1575	0.0	19.498413	2
TAGAGTC	80	1.6146923E-5	18.5	5
>>END_MODULE