FastQCFastQC Report
Fri 10 Feb 2017
ERR1631974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557831
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17200.30833711285317594No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17110.30672372098359535No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16340.2929202572105172No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15950.28592889244233466No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15500.27786193309443186No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15460.2771448700412849No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13220.23698933906505731No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12940.23196989769302892No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11980.21476038441750278No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11720.2100994745720478No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11370.20382517285701227No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11350.20346664133043876No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8750.15685754287588893No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8750.15685754287588893No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8230.14753572318497896No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8040.1441296736825311No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7980.1430540791028107No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7700.13803463773078226No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7690.13785537196749553No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7530.13498711975490785No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7320.13122253872588652No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6840.12261778208812346No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT6740.12082512445525616No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT6700.12010806140210924No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6630.11885320105910213No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6520.11688127766294809No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA6450.11562641731994099No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6440.11544715155665426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6360.11401302545036042No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6350.11383375968707368No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6320.1132959623972135No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6280.11257889934406656No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6220.1115033047643462No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6190.110965507474486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.11060697594791254No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA6150.11024844442133908No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6090.1091728498416187No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6080.10899358407833197No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6020.10791798949861157No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5910.10594606610245755No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5870.10522900304931063No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5850.10487047152273718No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5710.10236075083672296No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5710.10236075083672296No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5680.10182295354686276No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5610.10056809320385565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.029.3823531
CGTTATT701.9192339E-723.78571310
ATTAAGC400.001929947823.1250022
ATGTCTA400.001929947823.12500214
GTTCGAT400.001929947823.12500235
ATGCTAG502.6996862E-422.215
TGTTACG753.7336213E-722.19999916
CAGTCGG2200.021.86363610
CTCTATG3500.021.1428571
TAATACG6400.021.10156227
TGCGGGT4600.020.91304421
TTGTTAC806.9469024E-720.812515
GTGATTC2400.020.81249816
TCAATGA4200.020.70238132
CATTGTT450.00382337620.55555717
TTCGATT450.00382337620.55555736
TCTATGG3600.020.5555572
ATACGGC1351.8189894E-1220.55555529
GTAACGT4700.020.46808626
GGTGATT2550.020.31372315
Produced by FastQC (version 0.11.5)