FastQCFastQC Report
Fri 10 Feb 2017
ERR1631973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287554
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6840.2378683655939406No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6100.21213406873143828No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5800.20170124567907244No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5390.1874430541741725No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5120.17805351342704326No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4580.1592744319327848No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4250.1477983265751824No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4180.14536400119629705No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4040.14049535043852635No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3980.13840878582805316No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3850.13388789583869465No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3830.13319237430187025No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3050.10606703436571914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.031.477611
TGCGAAA358.8531646E-426.4285721
TAAGACT601.3315876E-624.6666664
GCGGTAA3050.024.26229523
GTATCAA4350.024.2413772
GCGAACT551.8962335E-523.54545423
GTTCGGC400.001927686323.12500235
CTCTATG802.7077476E-823.1250021
TTTTACG400.001927686323.1250024
TAATACG3150.022.90476227
CGCGGTA3300.022.42424222
CTTATAC2150.022.37209337
AGACTTC502.69528E-422.26
CGAACTA502.69528E-422.224
GGTAATA3400.021.76470625
CGGTAAT3400.021.76470624
TCTATGG855.1466486E-821.7647062
CGTGCCA3500.021.67142910
AATACGG3350.021.53731228
TGCGGGT1550.021.4838721
Produced by FastQC (version 0.11.5)