##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631973.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287554
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.253764510318064	33.0	31.0	34.0	30.0	34.0
2	32.43262830633551	34.0	31.0	34.0	31.0	34.0
3	32.52070567615126	34.0	31.0	34.0	31.0	34.0
4	36.07016421263484	37.0	35.0	37.0	35.0	37.0
5	35.916394833666025	37.0	35.0	37.0	35.0	37.0
6	35.955455323174085	37.0	35.0	37.0	35.0	37.0
7	35.95098659729999	37.0	35.0	37.0	35.0	37.0
8	35.925617449244314	37.0	35.0	37.0	35.0	37.0
9	37.71693664494321	39.0	37.0	39.0	35.0	39.0
10	37.59644101629607	39.0	37.0	39.0	35.0	39.0
11	37.6788950944866	39.0	37.0	39.0	35.0	39.0
12	37.602130382467294	39.0	37.0	39.0	35.0	39.0
13	37.67642251542319	39.0	37.0	39.0	35.0	39.0
14	38.959451094403136	40.0	38.0	41.0	36.0	41.0
15	38.94754376569271	40.0	38.0	41.0	36.0	41.0
16	38.8896589857905	40.0	38.0	41.0	36.0	41.0
17	38.85780062179625	40.0	38.0	41.0	36.0	41.0
18	38.86529486635553	40.0	38.0	41.0	36.0	41.0
19	38.88383051531191	40.0	38.0	41.0	35.0	41.0
20	38.87289691675303	40.0	38.0	41.0	35.0	41.0
21	38.83367993489919	40.0	38.0	41.0	35.0	41.0
22	38.80574431237263	40.0	38.0	41.0	35.0	41.0
23	38.73640777036661	40.0	38.0	41.0	35.0	41.0
24	38.75614667158168	40.0	38.0	41.0	35.0	41.0
25	38.68914708193939	40.0	38.0	41.0	35.0	41.0
26	38.59817286492276	40.0	38.0	41.0	35.0	41.0
27	38.477030401246374	40.0	38.0	41.0	34.0	41.0
28	38.47502382161264	40.0	38.0	41.0	34.0	41.0
29	38.4350174228145	40.0	38.0	41.0	34.0	41.0
30	38.39743143896451	40.0	38.0	41.0	34.0	41.0
31	38.30573735715726	40.0	38.0	41.0	34.0	41.0
32	38.254237464963104	40.0	38.0	41.0	34.0	41.0
33	38.19455823949588	40.0	38.0	41.0	34.0	41.0
34	38.14845559442748	40.0	38.0	41.0	34.0	41.0
35	38.05767264583348	40.0	37.0	41.0	33.0	41.0
36	38.02148813788019	40.0	37.0	41.0	33.0	41.0
37	37.96720615953873	40.0	37.0	41.0	33.0	41.0
38	37.92961322047337	40.0	37.0	41.0	33.0	41.0
39	37.853133672284166	40.0	37.0	41.0	33.0	41.0
40	37.547907523456466	40.0	37.0	41.0	33.0	41.0
41	37.62179973152869	40.0	37.0	41.0	33.0	41.0
42	37.60606355675804	40.0	36.0	41.0	33.0	41.0
43	37.08727752004841	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	3.0
18	3.0
19	13.0
20	16.0
21	36.0
22	77.0
23	154.0
24	240.0
25	387.0
26	610.0
27	938.0
28	1446.0
29	1987.0
30	2862.0
31	3850.0
32	5190.0
33	6797.0
34	9776.0
35	14305.0
36	22308.0
37	39907.0
38	86169.0
39	90479.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.12929049848028	16.418829159044908	12.493305605208064	27.95857473726674
2	21.005098172864923	19.516682084060733	32.010683210805624	27.46753653226872
3	21.18036960014467	19.80288919646397	28.965689922588457	30.05105128080291
4	15.923270064057535	15.656537554685382	33.56899921406066	34.85119316719642
5	16.84170625343414	33.020928243043045	33.52309479263025	16.614270710892562
6	34.998991493771605	33.60377529090188	15.061518879932118	16.335714335394396
7	30.576865562642148	27.70505713709425	19.74933403812849	21.968743262135114
8	27.768349596945267	30.776132482942337	20.07449035659389	21.381027563518504
9	28.479172607579795	12.59067862036348	17.32752804690597	41.60262072515075
10	19.120930329607656	23.567051753757557	29.915772341890566	27.39624557474422
11	39.60786495753841	19.28785549844551	18.703617407513022	22.40066213650306
12	23.160519415483698	23.451247417876296	26.5800510512808	26.8081821153592
13	35.02716011601299	17.526447206437748	20.781487998775884	26.664904678773375
14	24.32586575043296	19.518768648671205	22.85935859003874	33.296007010857096
15	29.289802958748616	24.01774970961976	20.050842624341865	26.641604707289762
16	26.087274042440722	23.938460254421777	21.918665711483758	28.05559999165374
17	27.09299818468879	23.67242326658645	21.691925690479007	27.542652858245752
18	26.884689484409886	21.5006572678523	23.208858162292994	28.40579508544482
19	28.227741572017777	22.20765491003429	22.934822676784187	26.629780841163747
20	29.798229202167242	20.88059981777336	22.517161993921142	26.804008986138257
21	27.523873776751493	22.073419253427183	21.610897431438964	28.791809538382356
22	28.49099647370581	22.709125938084675	21.106644317241283	27.693233270968236
23	28.25347586888028	22.12697441176266	22.005953664355214	27.613596055001842
24	28.113328279210165	21.698533145078837	22.232693685359965	27.95544489035103
25	28.385624960876914	21.793819595623777	22.637487219791762	27.183068223707547
26	28.14914763835662	22.38431738038768	22.18748478546638	27.27905019578931
27	27.161854816834403	21.73539578653053	23.565660710683904	27.53708868595116
28	26.75740904317102	22.80580343170326	21.91797018994693	28.51881733517878
29	27.21193236748576	23.28710433518574	22.062986430374817	27.437976866953683
30	27.142032453034908	22.206959388497467	23.119831405579475	27.531176752888154
31	27.959270258803564	22.553676874604424	22.458738184827894	27.028314681764122
32	25.909568289782094	22.294247341368926	22.686173727369468	29.110010641479512
33	26.19577540218533	22.02125513816535	24.015315384240875	27.767654075408444
34	26.774797081591633	21.850852361643376	23.54757715072647	27.82677340603852
35	26.141524722313026	23.315272957427126	24.077216801018245	26.465985519241602
36	26.416951250895483	22.197222086981924	24.46010140704007	26.925725255082522
37	27.054744500163448	21.802513614834083	23.95480501053715	27.18793687446532
38	25.922783198981758	21.805295700981382	25.113891651654995	27.15802944838187
39	25.760726680901673	20.772793979565577	25.75029385784931	27.716185481683443
40	25.393143548689984	21.490572205568345	26.60787191275378	26.50841233298789
41	23.74719183179507	20.823219290985346	27.468579814573957	27.961009062645626
42	22.55958880766743	21.152896499440104	28.44161444459128	27.845900248301188
43	22.078287904184954	21.554212426187778	28.213831141281293	28.15366852834598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	3.5
4	5.0
5	5.0
6	7.5
7	10.0
8	16.5
9	23.0
10	29.0
11	35.0
12	35.0
13	65.0
14	95.0
15	190.0
16	285.0
17	323.5
18	362.0
19	362.0
20	398.5
21	435.0
22	409.0
23	383.0
24	467.0
25	551.0
26	551.0
27	690.0
28	829.0
29	1212.5
30	1596.0
31	1910.5
32	2225.0
33	2225.0
34	2839.5
35	3454.0
36	4025.5
37	4597.0
38	5850.5
39	7104.0
40	7104.0
41	8363.5
42	9623.0
43	11019.0
44	12415.0
45	16396.0
46	20377.0
47	20377.0
48	22357.0
49	24337.0
50	27469.0
51	30601.0
52	31938.5
53	33276.0
54	33276.0
55	30407.5
56	27539.0
57	26119.5
58	24700.0
59	23316.0
60	21932.0
61	21932.0
62	20303.5
63	18675.0
64	15799.5
65	12924.0
66	11327.0
67	9730.0
68	9730.0
69	8299.5
70	6869.0
71	5866.5
72	4864.0
73	3939.5
74	3015.0
75	3015.0
76	2430.5
77	1846.0
78	1536.5
79	1227.0
80	973.5
81	720.0
82	720.0
83	565.0
84	410.0
85	330.0
86	250.0
87	191.5
88	133.0
89	133.0
90	100.5
91	68.0
92	46.5
93	25.0
94	15.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	287554.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.99034444437819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.81865579467994	54.17080410004195
2	5.502109395074366	6.711510943524698
3	1.7159501579187977	3.1396917354255773
4	0.8769408053186033	2.1393968709484805
5	0.5647504146360708	1.7222161156879676
6	0.3740897988761971	1.3689519411952453
7	0.29748550196600376	1.270062026248072
8	0.21438634040317553	1.0460397396287509
9	0.1804365287625643	0.9904397435619071
>10	1.2795142970589268	15.045414180093891
>50	0.11407854889956927	4.806720130351138
>100	0.05875045268327818	6.5711903120939885
>500	0.002851963722489232	1.017562161198312
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	684	0.2378683655939406	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	610	0.21213406873143828	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	580	0.20170124567907244	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	539	0.1874430541741725	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	512	0.17805351342704326	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	458	0.1592744319327848	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	425	0.1477983265751824	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	418	0.14536400119629705	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	404	0.14049535043852635	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	398	0.13840878582805316	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	385	0.13388789583869465	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	383	0.13319237430187025	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	305	0.10606703436571914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	3.4776076841219386E-4
12	0.0	0.0	0.0	0.0	3.4776076841219386E-4
13	0.0	3.4776076841219386E-4	0.0	0.0	3.4776076841219386E-4
14	0.0	3.4776076841219386E-4	0.0	0.0	3.4776076841219386E-4
15	0.0	3.4776076841219386E-4	0.0	3.4776076841219386E-4	3.4776076841219386E-4
16	0.0	3.4776076841219386E-4	0.0	6.955215368243877E-4	3.4776076841219386E-4
17	0.0	3.4776076841219386E-4	0.0	0.0010432823052365816	3.4776076841219386E-4
18	0.0	3.4776076841219386E-4	0.0	0.0010432823052365816	3.4776076841219386E-4
19	0.0	3.4776076841219386E-4	0.0	0.0013910430736487755	3.4776076841219386E-4
20	0.0	3.4776076841219386E-4	0.0	0.0017388038420609695	3.4776076841219386E-4
21	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.0020865646104731633	3.4776076841219386E-4
22	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.003129846915709745	3.4776076841219386E-4
23	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.0038253684525341326	3.4776076841219386E-4
24	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.005911933063007296	3.4776076841219386E-4
25	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.005911933063007296	3.4776076841219386E-4
26	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.008694019210304847	3.4776076841219386E-4
27	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.02677757916773893	3.4776076841219386E-4
28	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.09285212516605576	3.4776076841219386E-4
29	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.20309228875272123	3.4776076841219386E-4
30	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.3418488353491866	3.4776076841219386E-4
31	3.4776076841219386E-4	3.4776076841219386E-4	0.0	0.7341229821181413	3.4776076841219386E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	335	0.0	31.47761	1
TGCGAAA	35	8.8531646E-4	26.42857	21
TAAGACT	60	1.3315876E-6	24.666666	4
GCGGTAA	305	0.0	24.262295	23
GTATCAA	435	0.0	24.241377	2
GCGAACT	55	1.8962335E-5	23.545454	23
GTTCGGC	40	0.0019276863	23.125002	35
CTCTATG	80	2.7077476E-8	23.125002	1
TTTTACG	40	0.0019276863	23.125002	4
TAATACG	315	0.0	22.904762	27
CGCGGTA	330	0.0	22.424242	22
CTTATAC	215	0.0	22.372093	37
AGACTTC	50	2.69528E-4	22.2	6
CGAACTA	50	2.69528E-4	22.2	24
GGTAATA	340	0.0	21.764706	25
CGGTAAT	340	0.0	21.764706	24
TCTATGG	85	5.1466486E-8	21.764706	2
CGTGCCA	350	0.0	21.671429	10
AATACGG	335	0.0	21.537312	28
TGCGGGT	155	0.0	21.48387	21
>>END_MODULE