FastQCFastQC Report
Fri 10 Feb 2017
ERR1631972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339197
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5980.17629872905715557No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4360.1285388726905014No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT3940.11615668770655402No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3630.10701745593268809No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3610.10642782807630964No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3460.10200561915347128No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3410.10053154951252517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT200.001840107137.010
TGTTACG200.001840107137.016
CCGTTTA303.5941773E-430.83333227
ATTAGAT250.005490948429.63
GGTATCA2350.025.9787241
TACACTG451.3205271E-424.6666665
GACCGTT400.001928396223.1250027
CGACTTA400.001928396223.12500214
ATAGTAC400.001928396223.1250023
AACAATA502.6966626E-422.223
GCCGTTT502.6966626E-422.226
TAGGGTC603.7163256E-521.5833324
CGGTCGA603.7163256E-521.58333210
GCGTTAG450.003820332720.55555523
GTTACGG450.003820332720.55555517
TTTATAA450.003820332720.5555552
TATAAAG450.003820332720.5555554
ATCTTGA555.132826E-420.1818181
CTTATAC3250.019.92307937
TATGGGC759.234227E-619.7333344
Produced by FastQC (version 0.11.5)