FastQCFastQC Report
Fri 10 Feb 2017
ERR1631971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599762
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10040.16739973522830723No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9830.16389834634404982No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9600.16006349185176788No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9500.15839616381164529No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8290.13822149452616203No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8280.13805476172214978No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8040.1340531744258556No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7220.12038108449685042No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7210.12021435169283816No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7090.11821355804469105No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6880.11471216916043364No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6710.11187771149222525No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6460.1077093913919188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.10037314801537944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6020.10037314801537944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.031.01
TAGCACT502.700015E-422.24
ATACACA957.1286195E-921.42105337
TCTTACC705.0952112E-621.1428592
TAATACT806.948703E-720.81254
CTTATAC6000.020.65833337
GTTTATA450.003823706920.5555551
GTCAACC450.003823706920.55555515
TACCGCT450.003823706920.55555535
AACGACC1001.2867531E-820.3537
GTTACGG555.139154E-420.18181817
ATAAGAG555.139154E-420.18181813
CGCCGTT555.139154E-420.18181825
TATACAC1900.019.47368437
GGACCGT1153.0413503E-919.3043486
CTTAGGA1254.110916E-1019.242
GTATCAA11800.019.1271172
GCGGTAA4200.018.50000223
ATGCTAG801.6145705E-518.515
GGACGTA500.00703105618.527
Produced by FastQC (version 0.11.5)