##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631971.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599762
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60761268636559	34.0	31.0	34.0	31.0	34.0
2	32.76870158496204	34.0	31.0	34.0	31.0	34.0
3	32.866928881789775	34.0	31.0	34.0	31.0	34.0
4	36.29825997645733	37.0	37.0	37.0	35.0	37.0
5	36.20824427022719	37.0	35.0	37.0	35.0	37.0
6	36.25412913789136	37.0	37.0	37.0	35.0	37.0
7	36.24053707970828	37.0	37.0	37.0	35.0	37.0
8	36.2234002821119	37.0	37.0	37.0	35.0	37.0
9	38.054604993314015	39.0	38.0	39.0	35.0	39.0
10	37.98291488957286	39.0	38.0	39.0	35.0	39.0
11	38.05744612029439	39.0	38.0	39.0	35.0	39.0
12	37.99632854365565	39.0	38.0	39.0	35.0	39.0
13	38.04614330351039	39.0	38.0	39.0	35.0	39.0
14	39.44828448617952	40.0	39.0	41.0	37.0	41.0
15	39.446577142266435	41.0	39.0	41.0	37.0	41.0
16	39.39384622566951	40.0	39.0	41.0	37.0	41.0
17	39.3583321384149	40.0	39.0	41.0	36.0	41.0
18	39.37604249685709	40.0	39.0	41.0	36.0	41.0
19	39.40223622036741	40.0	39.0	41.0	37.0	41.0
20	39.386403273298406	40.0	39.0	41.0	37.0	41.0
21	39.350565724404014	40.0	39.0	41.0	36.0	41.0
22	39.3335939922836	40.0	39.0	41.0	36.0	41.0
23	39.267312700704615	40.0	39.0	41.0	36.0	41.0
24	39.276554700031014	40.0	39.0	41.0	36.0	41.0
25	39.219445380000735	40.0	39.0	41.0	36.0	41.0
26	39.15658211090399	40.0	39.0	41.0	36.0	41.0
27	39.054906779689276	40.0	39.0	41.0	35.0	41.0
28	39.037048029051526	40.0	39.0	41.0	35.0	41.0
29	39.018048826034324	40.0	39.0	41.0	35.0	41.0
30	38.97200556220634	40.0	39.0	41.0	35.0	41.0
31	38.894613196567974	40.0	38.0	41.0	35.0	41.0
32	38.84027831039646	40.0	38.0	41.0	35.0	41.0
33	38.78465791430601	40.0	38.0	41.0	35.0	41.0
34	38.7579289784948	40.0	38.0	41.0	35.0	41.0
35	38.68887325305705	40.0	38.0	41.0	35.0	41.0
36	38.640594102327256	40.0	38.0	41.0	35.0	41.0
37	38.60554853425192	40.0	38.0	41.0	35.0	41.0
38	38.56133933126807	40.0	38.0	41.0	35.0	41.0
39	38.4931889649561	40.0	38.0	41.0	35.0	41.0
40	38.18077504076617	40.0	38.0	41.0	34.0	41.0
41	38.246901270837434	40.0	37.0	41.0	34.0	41.0
42	38.23825117296528	40.0	37.0	41.0	34.0	41.0
43	37.7571019837869	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	1.0
18	10.0
19	10.0
20	32.0
21	54.0
22	104.0
23	206.0
24	341.0
25	547.0
26	842.0
27	1281.0
28	1891.0
29	2881.0
30	4045.0
31	5488.0
32	7709.0
33	10540.0
34	15021.0
35	22279.0
36	35159.0
37	62949.0
38	143240.0
39	285129.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.111767667841576	16.364657980999127	12.482451372377708	28.041122978781587
2	20.27437550228257	19.446880595969734	33.00725954628669	27.271484355461002
3	20.82959573964339	20.006769351842898	29.078701218149867	30.084933690363847
4	15.52299078634525	15.823443299175338	33.59132455874164	35.062241355737775
5	16.673780599637855	33.05511186103821	33.660685405210735	16.610422134113197
6	34.49601675331215	33.6630196644669	15.4461269636956	16.39483661852535
7	30.74319480060424	27.881726418145863	20.09330367712526	21.281775104124637
8	27.239471657090647	31.345266955892505	20.125816573907652	21.289444813109203
9	28.348078070968153	13.108032853031704	17.73336756913576	40.81052150686439
10	18.980695675951463	23.855129201249827	30.33119804188995	26.83297708090876
11	38.383392078857945	19.875217169477228	19.248968757607184	22.492421994057644
12	22.926760948509575	23.767594479143394	26.807800427502908	26.497844144844123
13	33.92395650274609	17.95645606090416	21.621243093093597	26.498344343256157
14	24.205601555283597	19.765173518829133	23.3147481834461	32.714476742441164
15	28.90029711785675	24.323648380524272	20.342402486319575	26.4336520152994
16	25.890436539827466	24.22527602615704	22.54961134583385	27.33467608818165
17	26.561035877564766	23.984680589967354	22.32002027470897	27.13426325775891
18	26.14053574584585	22.21898019547754	23.880806053067715	27.75967800560889
19	27.804362397084176	22.602632377509746	23.59519275979472	25.997812465611357
20	29.071364974773328	21.49986161177267	23.256891900453848	26.171881513000155
21	27.038558628255878	22.51943270830763	22.486419613113203	27.955589050323294
22	27.7801861405024	23.112167826571206	21.88751538110117	27.220130651825226
23	27.387697119857545	22.67082609435076	22.90491895118397	27.036557834607727
24	27.409872582791174	22.26633231181702	23.007959824063544	27.315835281328262
25	27.575104791567323	22.307015115996013	23.40545082882877	26.712429263607895
26	27.460892820818927	23.229547720595836	22.836558501538946	26.473000957046295
27	27.176613389978026	22.098932576588716	23.697733434262258	27.026720599171007
28	26.253080388554128	23.135677151936935	22.65615360759768	27.95508885191126
29	26.896669012041443	23.46147305097689	22.86640367345714	26.775454263524534
30	26.68441815253384	22.707674044037468	23.722576622060085	26.88533118136861
31	27.34651411726652	22.87490704646176	23.04830916263451	26.73026967363721
32	25.37006345850521	22.770865776758114	23.51932933396914	28.33974143076754
33	25.622330190975756	22.42372807880459	24.746482771499362	27.20745895872029
34	26.380297518015478	22.240321994391106	24.20276709761539	27.176613389978026
35	25.808904198665473	23.48098078904632	24.5470703379007	26.16304467438751
36	25.86292562716544	22.576788792887843	25.02275902774767	26.537526552199036
37	26.382131578859614	22.16512549978158	24.689960350939206	26.762782570419603
38	25.533461606437218	21.714946928948482	25.74037701621643	27.011214448397862
39	25.010420800250767	21.154057776251246	26.42531537509879	27.4102060483992
40	24.69763005992377	21.7981465981506	27.112254527629293	26.391968814296334
41	23.3582654452933	21.02567351716181	28.215358758974396	27.4007022785705
42	22.48558594909314	21.397320937305132	28.52181365274892	27.595279460852808
43	21.792144217206157	21.820655526692253	28.568332105068343	27.818868151033243
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	4.5
2	1.0
3	11.0
4	21.0
5	21.0
6	33.5
7	46.0
8	47.5
9	49.0
10	89.0
11	129.0
12	129.0
13	242.5
14	356.0
15	719.5
16	1083.0
17	1070.5
18	1058.0
19	1058.0
20	1246.0
21	1434.0
22	1280.0
23	1126.0
24	1333.0
25	1540.0
26	1540.0
27	1841.0
28	2142.0
29	3007.5
30	3873.0
31	4919.0
32	5965.0
33	5965.0
34	7503.0
35	9041.0
36	10419.5
37	11798.0
38	14684.5
39	17571.0
40	17571.0
41	20502.5
42	23434.0
43	26427.5
44	29421.0
45	36847.0
46	44273.0
47	44273.0
48	47872.5
49	51472.0
50	58053.5
51	64635.0
52	66494.0
53	68353.0
54	68353.0
55	62234.0
56	56115.0
57	52432.0
58	48749.0
59	45025.5
60	41302.0
61	41302.0
62	37985.0
63	34668.0
64	29414.0
65	24160.0
66	21067.0
67	17974.0
68	17974.0
69	15444.0
70	12914.0
71	11225.5
72	9537.0
73	7814.0
74	6091.0
75	6091.0
76	4948.5
77	3806.0
78	3166.0
79	2526.0
80	1936.5
81	1347.0
82	1347.0
83	1084.5
84	822.0
85	662.0
86	502.0
87	378.5
88	255.0
89	255.0
90	184.0
91	113.0
92	79.0
93	45.0
94	26.0
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	599762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.36170623399524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93764302486535	44.9984161850995
2	6.924012601067419	7.2510622755514715
3	2.2669576784262664	3.5610531600796795
4	1.0985313226625544	2.3008389762439565
5	0.7139006202521159	1.8690527278954145
6	0.4620652676373886	1.4516715482976799
7	0.3330909769690488	1.2208848319673467
8	0.2553495802590014	1.069643176679666
9	0.21062076139939193	0.9925616191657829
>10	1.5001677286595374	15.71817416775847
>50	0.1785962637209395	6.470372192520103
>100	0.11362306935142306	10.957969359599918
>500	0.005121039745416245	1.9700379264677979
>1k	3.200649840885153E-4	0.16826185267321728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1004	0.16739973522830723	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	983	0.16389834634404982	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	960	0.16006349185176788	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	950	0.15839616381164529	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	829	0.13822149452616203	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	828	0.13805476172214978	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	804	0.1340531744258556	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	722	0.12038108449685042	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	721	0.12021435169283816	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	709	0.11821355804469105	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	688	0.11471216916043364	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	671	0.11187771149222525	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	646	0.1077093913919188	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.10037314801537944	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.10037314801537944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.667328040122582E-4	0.0
5	0.0	0.0	0.0	1.667328040122582E-4	0.0
6	0.0	0.0	0.0	1.667328040122582E-4	0.0
7	0.0	0.0	0.0	1.667328040122582E-4	0.0
8	0.0	0.0	0.0	1.667328040122582E-4	0.0
9	0.0	0.0	0.0	1.667328040122582E-4	0.0
10	0.0	0.0	0.0	1.667328040122582E-4	0.0
11	0.0	0.0	1.667328040122582E-4	1.667328040122582E-4	0.0
12	0.0	0.0	1.667328040122582E-4	3.334656080245164E-4	0.0
13	0.0	0.0	1.667328040122582E-4	3.334656080245164E-4	0.0
14	0.0	0.0	1.667328040122582E-4	6.669312160490328E-4	0.0
15	1.667328040122582E-4	0.0	1.667328040122582E-4	6.669312160490328E-4	0.0
16	1.667328040122582E-4	0.0	1.667328040122582E-4	0.0011671296280858074	0.0
17	1.667328040122582E-4	0.0	1.667328040122582E-4	0.001667328040122582	0.0
18	1.667328040122582E-4	0.0	1.667328040122582E-4	0.001667328040122582	0.0
19	1.667328040122582E-4	0.0	1.667328040122582E-4	0.0018340608441348401	0.0
20	1.667328040122582E-4	0.0	1.667328040122582E-4	0.0021675264521593567	0.0
21	1.667328040122582E-4	0.0	1.667328040122582E-4	0.002667724864196131	0.0
22	3.334656080245164E-4	0.0	1.667328040122582E-4	0.005001984120367746	0.0
23	3.334656080245164E-4	0.0	1.667328040122582E-4	0.006169113748453553	0.0
24	3.334656080245164E-4	0.0	1.667328040122582E-4	0.007669708984563877	0.0
25	3.334656080245164E-4	0.0	1.667328040122582E-4	0.008503373004625168	0.0
26	3.334656080245164E-4	0.0	1.667328040122582E-4	0.009837235436723234	0.0
27	3.334656080245164E-4	0.0	1.667328040122582E-4	0.027510912662022602	0.0
28	3.334656080245164E-4	0.0	1.667328040122582E-4	0.10454146811568589	0.0
29	3.334656080245164E-4	1.667328040122582E-4	1.667328040122582E-4	0.2280904758887692	0.0
30	3.334656080245164E-4	1.667328040122582E-4	1.667328040122582E-4	0.38782050213251257	0.0
31	3.334656080245164E-4	1.667328040122582E-4	1.667328040122582E-4	0.8568398798189949	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	31.0	1
TAGCACT	50	2.700015E-4	22.2	4
ATACACA	95	7.1286195E-9	21.421053	37
TCTTACC	70	5.0952112E-6	21.142859	2
TAATACT	80	6.948703E-7	20.8125	4
CTTATAC	600	0.0	20.658333	37
GTTTATA	45	0.0038237069	20.555555	1
GTCAACC	45	0.0038237069	20.555555	15
TACCGCT	45	0.0038237069	20.555555	35
AACGACC	100	1.2867531E-8	20.35	37
GTTACGG	55	5.139154E-4	20.181818	17
ATAAGAG	55	5.139154E-4	20.181818	13
CGCCGTT	55	5.139154E-4	20.181818	25
TATACAC	190	0.0	19.473684	37
GGACCGT	115	3.0413503E-9	19.304348	6
CTTAGGA	125	4.110916E-10	19.24	2
GTATCAA	1180	0.0	19.127117	2
GCGGTAA	420	0.0	18.500002	23
ATGCTAG	80	1.6145705E-5	18.5	15
GGACGTA	50	0.007031056	18.5	27
>>END_MODULE